Effect of dietary genistein on growth performance, digestive enzyme activity, and body composition of Nile tilapia Oreochromis niloticus

Dong Chen; Wei Wang; Shaoguo Ru

doi:10.1007/s00343-015-4037-6

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Effect of dietary genistein on growth performance, digestive enzyme activity, and body composition of Nile tilapia Oreochromis niloticus

OriginalPaper | Updated：2023-05-16

- Effect of dietary genistein on growth performance, digestive enzyme activity, and body composition of Nile tilapia Oreochromis niloticus
- Effect of dietary genistein on growth performance, digestive enzyme activity, and body composition of Nile tilapia Oreochromis niloticus
- 海洋湖沼学报（英文） 2015年33卷第1期页码：77-83
- Affiliations：
  
  1. College of Marine Life Sciences Ocean University of China
  2. School of Environmental and Municipal Engineering Qingdao Technological University
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 31170322)
- DOI：10.1007/s00343-015-4037-6
  中图分类号：
- 网络首发：2014-10-21，
  
  纸质出版：2015
- Accepted：
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Effect of dietary genistein on growth performance, digestive enzyme activity, and body composition of Nile tilapia Oreochromis niloticus[J]. 海洋湖沼学报（英文）, 2015,33(1):77-83.

Chen, D., Wang, W. & Ru, S. Effect of dietary genistein on growth performance, digestive enzyme activity, and body composition of Nile tilapia Oreochromis niloticus, ., Chin. J. Ocean. Limnol. 33, 77–83 (2015). https://doi.org/10.1007/s00343-015-4037-6
Effect of dietary genistein on growth performance, digestive enzyme activity, and body composition of Nile tilapia Oreochromis niloticus[J]. 海洋湖沼学报（英文）, 2015,33(1):77-83. DOI： 10.1007/s00343-015-4037-6.

Chen, D., Wang, W. & Ru, S. Effect of dietary genistein on growth performance, digestive enzyme activity, and body composition of Nile tilapia Oreochromis niloticus, ., Chin. J. Ocean. Limnol. 33, 77–83 (2015). https://doi.org/10.1007/s00343-015-4037-6 DOI：

摘要

Abstract

An 8-week feeding experiment was performed to evaluate the effect of dietary genistein on growth performance

body composition

and digestive enzymes activity of juvenile Nile tilapia (Oreochromis niloticus). Four isonitrogenous and isoenergetic diets were formulated containing four graded supplements of genistein: 0

300

and 3 000 μg/g. Each diet was randomly assigned in triplicate to tanks stocked with 15 juvenile tilapia (10.47±1.24 g). The results show that 30 and 300 μg/g dietary genistein had no significant effect on growth performance of Nile tilapia

but the higher level of genistein (3 000 μg/g) significantly depressed the final body weight and specific growth rate. There was no significant difference in survival rate

feed intake

feed efficiency ratio or whole body composition among all dietary treatments. An assay of digestive enzymes showed that the diet containing 3 000 μg/ggenistein decreased stomach and hepatopancreas protease activity

and amylase activity in the liver and intestine

while a dietary level of 300 μg/g genistein depressed stomach protease and intestine amylase activities. However

no significant difference in stomach amylase activity was found among dietary treatments. Overall

the results of the present study indicate that a high level of dietary genistein (3 000 μg/g

or above) would significantly reduce the growth of Nile tilapia

partly because of its inhibitory effect on the activity of major digestive enzymes. Accordingly

the detrimental effects of genistein

as found in soybean products

should not be ignored when applied as an alternative ingredient source in aquaculture.

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references

Abel H J, Becker K, Meske C H R, Friedrich W. 1984. Possibilities of using heat-treated full-fat soybeans in carp feeding. Aquaculture, 42:97–108.

Ai Q H, Mai K S, Li H T et al. 2004. Effects of dietary protein to energy ratios on growth and body composition of juvenile Japanese seabass, Lateolabrax japonicus. Aquaculture, 230:507–516.

Ai Q H, Xie X J. 2002. The nutrition of S ilurus meridionalis: effects of different levels of dietary soybean protein on digestibility and feeding rate. Acta Hydrobiologica Sinica, 26(3): 215–220.

AOAC (Association of Official Analytical Chemists). 1995. Official Methods of Analysis of Official Analytical Chemists International, 16th ed. Association of Official Analytical Chemists, Arlington, VA.

Boyd E C. 2004. Farm-Level Issues in Aquaculture Certification: Tilapia. Report commissioned by WWF-US in 2004. Auburn University, Alabama 36831.

Bradford M M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry, 72:248–254.

Cai Y H. 2006. Effects of Antinutritional Factors in Soybean on the Growth Performance and Digestive Physiology of Japanese Flounder, Paralichthys olivaceus. MS thesis, Ocean University of China, Qingdao. 183p. (in Chinese with English abstract)

Carter C G, Hauler R C. 2000. Fish meal replacement by plant meals in extruded feeds for Atlantic salmon, Salmo salar. Aquaculture, 185:299–311.

Catherine B P, Bernard B B, Bernard B et al. 2001. Effect of genistein-enriched diets on the endocrine process of gametogenesis and on reproduction efficiency of the rainbow trout (Oncorhynchus mykiss). General and Comparative Endocrinology, 121:173–187.

Chen W. 2009. Effects of Antinutritional Factors on the Utilization of Soybean Proteins by Japanese Flounder, Paralichthys olivaceus. PhD thesis, Ocean University of China, Qingdao. (in Chinese with English abstract)

Dytham C. 1999. Choosing and Using Statistics: A Biologist’s Guide. Blackwell Science Ltd., London, UK.

El-Saidy D M S D, Gaber M M A. 2002. Complete replacement of fish meal by soybean meal with dietary L-lysine supplementation for Nile tilapia Oreochromis niloticus (L.) fingerlings. J. World Aquac. Soc., 33:297–306.

FAO. 2004. Tilapias as Alien Aquatics in Asia and the Pacific: A Review. FAO Fisheries Technical Papers-T453. ISBN: 9251052271. 74 p.

Francesco M, Parisi G, Medale F et al. 2004. Effect of longterm feeding with a plant protein mixture based diet on growth and body/fillet quality traits of large rainbow trout (Oncorhynchus mykiss). Aquaculture, 236:413–429.

Friendman M, Baron D L. 2001. Nutritional and health benefits of soy proteins. Journal of Agricultural and Food Chem., 49:1 069–1 086.

Han Z K, Wang G J. 1993. Studies of Phytoestrogen (Daidzein) Affecting the Growth and Sex Hormone Levels in Broiler. World Conference on Animal Production. Edmondon, Canada. p.152–153.

Hardy R W, Barrows F T. 2002. Diet formulation and manufacture. In: Halver J E, Hardy R W eds. Fish Nutrition, 3rd ed. Academic Press, San Diego, CA. p.505–600.

Ishibashi H, Kobayashi M, Koshiishi T et al. 2002. Induction of plasma vitellogenin synthesis by the commercial fish diets in male goldfish (Carassius auratus) and dietary phytoestrogens. J. Health Sci., 48:427–434.

Jiang S Q, Jiang Z Y. 2006. The research and application of soybean isoflavones in animal industry. Feed Industry, 27(12): 1–6. (in Chinese)

Kaushik S J, Cravedi J P, Lalles J P, Sumpter J, Fauconneau B, Laroche M. 1995. Partial or total replacement of fish meal by soybean protein on growth, protein utilization, potential estrogenic or antigenic effects, cholesterolemia and flesh quality in rainbow trout. Aquaculture, 133:257–274.

Kinarm K, Jeffrey A M, Jess D R. 1999. Effect of genistein on the growth and reproductive function of male and female Yellow Perch Perca flavescens. J. World Aquac. Soc., 30(1): 73–79.

Krogdahl A, Bakke-McKellep A M, Baeverfjord G. 2003. Effects of graded levels of standard soybean meal on intestinal structure, mucosal enzyme activities, and pancreatic response in Atlantic salmon (Salmo salar L.). Aquac. Nutr., 9:361–371.

Kuhnl G, Ren M, Sehneider F et al. 2002. Effects of daidzein supplementation to the diet of pregnant sows on maternal performance and neonatal piglet growth. J. Anim. Sci., 80(Suppl.1): 209.

Lemieux H, Blier P, Dutil J D. 1999. Do digestive enzymes set a physiological limit on growth rate and food conversion efficiency in the Atlantic cod (Gadus morhua). Fish Physiol. Biochem., 20:293–303.

Lilleeng E, Froystad M K, Vekterud K, Valen E C, Krogdahl A. 2007. Comparison of intestinal gene expression in Atlantic cod (Gadus morhua) fed standard fish meal or soybean meal by means of suppression subtractive hybridization and real-time PCR. Aquaculture, 267:269–283.

Lin S M, Luo L. 2011. Effects of different levels of soybean meal inclusion in replacement for fish meal on growth, digestive enzymes and transaminase activities in practical diets for juvenile tilapia, Oreochromis niloticus × O. aureus. Anim. Feed Sci. Technol., 168(1–2): 80–87.

Ma H T. 2005. The Study of Isoflavonic Phytoestrogen on the Growth Performance and the Absorption Mechanism of Animal. PhD thesis, Nanjing Agricultural University. Nanjing. 141p. (in Chinese with English abstract)

Mambrini M, Roem A, Cravedi J P, Lalles J P, Kaushik S J. 1999. Effects of replacing fish meal by soy protein concentrate and of DL-methionine supplementation in high energy extruded diets on growth and nutrient utilisation in rainbow trout, Oncorhynchus mykiss. Anim. Sci., 77:2 990–2 999.

Mai K S, Zhang Y J, Chen W, Xu W, Ai Q H, Zhang W B. 2012. Effects of dietary soy isoflavones on feed intake, growth performance and digestibility in juvenile Japanese flounder (Paralichthys olivaceus). J. Ocean Univ. China, 11(4): 511–516.

Mao S S. 2009. Effects of Genistein on Digestive Enzymes and Antioxidant Systems of Goldfish, Carassius auratus. MS thesis, Ocean University of China, Qingdao. 60p. (in Chinese with English abstract)

Mi H F. 2008. Effects of Different Protein Sources and Antinutritional Factors on Gene Expression and Activity of Protein-Digestive-Enzyme of Japanese Flounder, Paralichthys olivaceus. PhD thesis, Ocean University of China, Qingdao. 183p. (in Chinese with English abstract)

Natalia Y, Hashim R, Ali A, Chong A. 2004. Characterization of digestive enzymes in a carnivorous ornamental fish, the Asian bony tongue Scleropages formosus (Osteoglossidae). Aquaculture, 233:305–320.

NRC (National Research Council). 1993. Nutritional Requirements of Fish. National Academic Press, Washington, DC, USA. 114p.

Pollack S J, Ottinger M A, Sullivan C V et al. 2003. The effects of the soy isoflavone genistein on the reproductive development of striped bass. North American Journal of Aquaculture, 65:226–234.

Refstie S, Storebakken T. 2001. Vegetable protein source for carnivorous fish: potential and challenges. Recent Adv. Anim. Nutr. Australia, 13:195–203.

Robaina L, Izquierdo M S, Moyano F J et al. 1995. Soybean and lupin seed meals as protein sources in diets for gilthead seabream (Sparus aurata): nutritional and histological implications. Aquaculture, 130:219–233.

Rodriguez G A M de O. 2005. Evaluation of Dietary Phytochemicals on Sex Differentiation and Growth in Nile Tilapia (Oreochromis niloticus). MS thesis, Ohio State University, Columbus. 125p.

Rungruangsak T K. 2001. Important parameters for growth study. In: The 2nd International Symposium on Cultivation of Salmon II, Bergen, Norway, 7–10 May 2001. p.14 (abstract).

Siddhuraju P, Becker K. 2005. Nutritional and antinutritional composition, in vitro amino acid availability, starch digestibility and predicted glycemic index of differentially processed mucuna beans (Mucuna pruriens var. utilis): an under utilized legume. Food Chem., 91:275–286.

Storebakken T, Shearer K D, Roem A J. 1998. Availability of protein, phosphorus and other elements in fishmeal, soy protein concentrate and phytase-treated soy proteinconcentrate-based diets to Atlantic salmon, Salmo salar. Aquaculture, 161:365–379.

Sun B G. 2008. The effect of replacing fish meal by soybean meal on growth of carp. Inland Fisheries, 4:38–41. (in Chinese)

Tacon A G J. 1994. Feed Ingredients for Carnivorous Fish Species: Alternatives to Fishmeal and Other Fishery Resources. Food and Agricultural Organization, Rome, 1994.

Tepavcevic V, Atanackovic M, Miladinovic J et al. 2010. Isoflavone composition, total polyphenolic content, and antioxidant activity in soybeans of different origin. Journal of Medicinal Food, 13(3): 657–664.

Wang G Q, Li Q H. 2007. The effect of main antinutritional factors in soybean protein on fish. Feed Industry, 28(12): 44–47. (in Chinese)

Wojcik-Gładysz A, Romanowicz K, Misztal T et al. 2005. Effects of intracerebroventricular infusion of genistein on the secretory activity of the GnRH/LH axis in ovariectomized ewes. Animal Reproduction Science, 86:221–235.

Worthington V. 1993. Worthington Enzyme Manual. Enzymes and Related Biochemicals Worthington Chemical, New Jersey, US. 399p.

Xiao C W, Mei J, Wood C M. 2008. Effect of soy proteins and isoflavones on lipid metabolism and involved gene expression. Frontiers in Bioscience, 13:2 660–2 673.

Yamamoto T, Suzuki N, Furuita F, Sugita T, Tanaka N, Goto T. 2007. Supplemental effect of bile salts to soybean mealbased diet on growth and feed utilization of rainbow trout (Oncorhynchus mykiss). Fisheries Science, 73:123–131.

Zhang W. 2010. Effects of Soybean Saponins and Soybean Isoflavones on Growth, Physiology and Intestinal Health of Allogynogentic Silver Crucian Carp. MS thesis, Soochow University, Suzhou. 76p. (in Chinese with English abstract)

Zhang X Y, Wang G L, Tang X W, Huang J M. 2006. Effects of daidzein on body weight gain and serum hormone level in male piglets. Chinese Journal of Animal Nutrition, 18(1): 59–61. (in Chinese)

Zhao W. 2006. The Study of Skatole on Some Properties of Important Digestive Enzymes. MS thesis, Sichuan University, Chengdu. 73p. (in Chinese with English abstract)

Zhou Y C, Zhao R Q, Lu L Z et al. 2002. Effects of daidzein on egg laying performance and serum hormone levels in Shaoxing ducks during early and late laying period. Acta Zoonutrimenta Sinica, 14(2): 14–18.

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