Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature

Yifan Liu; Daoyuan Ma; Zhizhong Xiao; Shihong Xu; Yanfeng Wang; Yufu Wang; Yongshuang Xiao; Zongcheng Song; Zhaojun Teng; Qinghua Liu; Jun Li

doi:10.1007/s00343-015-4028-7

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Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature

OriginalPaper | Updated：2023-05-16

- Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature
- Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature
- 海洋湖沼学报（英文） 2015年33卷第1期页码：11-19
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
  3. Nantong Branch Institute of Oceanology Chinese Academy of Sciences
  4. Weihai Shenghang Aquatic Product Science and Technology Co. Ltd.
  5. Rizhao Proliferation Station of Marine Aquatic Resources
- Author bio：
- Funds：
  
  Supported by the Modern Agro-Industry Technology Research System of China (No. nycytx-50), the Key Innovation Program of Chinese Academy of Sciences, the Experiment and Demonstration of Scientific and Technical Innovation on Modern Ecological Ocean Agriculture (No. KSC2-EW-B-3), the Transformation Fund for Agricultural Science and Technology Achievements (No. 2013GB2C600263), the Science-Technology R&D Project of Shandong Province (No. 2011GHy11530), the Shandong Province Agricultural Seed Project (No. 2014–2016), the Jiangsu Provincial Natural Science Foundation of China (No. BK2012222), and the Fundamental Research Project of Technology Program of Qingdao, China (Nos. 12-1-4-8(6)-jch, 12-1-4-8-(7)-jch, 12-4-1-51-hy)
- DOI：10.1007/s00343-015-4028-7
  中图分类号：
- 网络首发：2014-10-27，
  
  纸质出版：2015
- Accepted：
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Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature[J]. 海洋湖沼学报（英文）, 2015,33(1):11-19.

Liu, Y., Ma, D., Xiao, Z. et al. Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature., Chin. J. Ocean. Limnol. 33, 11–19 (2015). https://doi.org/10.1007/s00343-015-4028-7
Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature[J]. 海洋湖沼学报（英文）, 2015,33(1):11-19. DOI： 10.1007/s00343-015-4028-7.

Liu, Y., Ma, D., Xiao, Z. et al. Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature., Chin. J. Ocean. Limnol. 33, 11–19 (2015). https://doi.org/10.1007/s00343-015-4028-7 DOI：

摘要

Abstract

High temperature influences the homeostasis of fish. We investigated the effects of elevated temperature on tissues of Japanese flounder (Paralichthys olivaceus) by analyzing the histology and heat shock protein 70 (hsp70) expression of fish reared in warm conditions. In this study

temperature was increased at 1±0.5°C/day starting at 24±0.5°C

and was kept at that temperature for 5 days before the next rise. After raising temperature at the rate up to 32±0.5°C

tissue samples from midgut

spleen

stomach

liver

muscle

gill

heart

trunk kidney and brain were collected for histological analysis and mRNA assay. Almost all the tissues showed changes in morphological structure and hsp70 level at 32±0.5°C. Histological assessment of the tissues indicated that the gill had the most serious damage

including highly severe epithelial lifting and edema

curved tips and hyperemia at the ending of the lamellars

desquamation and necrosis. The next most severe damage was found in liver and kidney. The hsp70 levels in all the tissues first increased and then decreased. The gut

stomach

muscle

heart

and brain had the highest expressions in 6 h

whereas the spleen

liver

gill and kidney had the highest expressions in 2 h. Therefore

tissues with the most significant lesions (especially gill and liver) responded much earlier (2 h) in hsp70 expression than other tissues

and these tissues demonstrated the most marked histological disruption and elevated mRNA levels

making them ideal candidates for further studies on the thermal physiology of this species.

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references

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