Effects of UV-B irradiation on isoforms of antioxidant enzymes and their activities in red alga Grateloupia filicina (Rhodophyta)

Jiqiang Zhao; Lixia Li

doi:10.1007/s00343-015-3366-9

Your Location：

Home >

Browse articles >

Effects of UV-B irradiation on isoforms of antioxidant enzymes and their activities in red alga Grateloupia filicina (Rhodophyta)

OriginalPaper | Updated：2023-05-16

- Effects of UV-B irradiation on isoforms of antioxidant enzymes and their activities in red alga Grateloupia filicina (Rhodophyta)
- Effects of UV-B irradiation on isoforms of antioxidant enzymes and their activities in red alga Grateloupia filicina (Rhodophyta)
- 海洋湖沼学报（英文） 2014年32卷第6期页码：1364-1372
- Affiliations：
  
  College of Life Sciences Yantai University
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 31300326) and the Natural Science Foundation of Shandong Province (Nos. ZR2010DQ007, ZR2011CM006)
- DOI：10.1007/s00343-015-3366-9
  中图分类号：
- 网络首发：2014-08-07，
  
  纸质出版：2014
- Accepted：
Scan QR Code
Effects of UV-B irradiation on isoforms of antioxidant enzymes and their activities in red alga Grateloupia filicina (Rhodophyta)[J]. 海洋湖沼学报（英文）, 2014,32(6):1364-1372.

Zhao, J., Li, L. Effects of UV-B irradiation on isoforms of antioxidant enzymes and their activities in red alga Grateloupia filicina (Rhodophyta)., Chin. J. Ocean. Limnol. 32, 1364–1372 (2014). https://doi.org/10.1007/s00343-015-3366-9
Effects of UV-B irradiation on isoforms of antioxidant enzymes and their activities in red alga Grateloupia filicina (Rhodophyta)[J]. 海洋湖沼学报（英文）, 2014,32(6):1364-1372. DOI： 10.1007/s00343-015-3366-9.

Zhao, J., Li, L. Effects of UV-B irradiation on isoforms of antioxidant enzymes and their activities in red alga Grateloupia filicina (Rhodophyta)., Chin. J. Ocean. Limnol. 32, 1364–1372 (2014). https://doi.org/10.1007/s00343-015-3366-9 DOI：

摘要

Abstract

Macroalgae in a littoral zone are inevitably exposed to UV-B irradiance. We analyzed the effects of UV-B on isoenzyme patterns and activities of superoxide dismutase (SOD)

peroxidase (POX)

catalase (CAT)

and ascorbate peroxidase (APX) of red algae Grateloupia filicina (Lamour.) C. Agardh. The activities of SOD

CAT

and APX changed in response to UV-B in a time- and dose-dependent manner. POX activity increased significantly under all three UV-B treatments. The enzymatic assay showed three distinct bands of SODI (Mn-SOD)

SODII (Fe-SOD)

and SODIII (CuZn-SOD) under a low (Luv) and medium (Muv) dose of UV-B irradiation

while SODI and SODIII activities decreased significantly when exposed to a high dose of UV-B irradiation (Huv). The activity of POX isoenzymes increased significantly after exposure to UV-B

which is consistent with the total activity. In addition

a clear decrease in activity of CATIV was detected in response to all the three doses of UV treatments. Some bands of APX isoenzyme were also clearly influenced by UV-B irradiation. Correspondingly

the daily growth rate declined under all the three exposure doses

and was especially significant under Muv and Huv treatments. These data suggest that

although the protection mechanisms of antioxidant defense system are partly inducible by UV-B to prevent the damage

G. filicina has incomplete tolerance to higher UV-B irradiation stress.

关键词

Keywords

references

Aguilera J, Dummermuth A, Karsten U, Schriek R, Wiencke C. 2002. Enzymatic defences against photooxidative stress induced by ultraviolet radiation in Arctic marine macroalgae. Polar Biol., 25(6): 432–441.

Aguilera J, Karsten U, Lippert H, Vögele B, Philipp E, Hanelt D, Wiencke C. 1999. Effects of solar radiation on growth, photosynthesis and respiration of marine macroalgae from the Arctic. Mar. Ecol. Prog. Ser., 191: 109–119.

Asada K. 1992. Minireview: ascorbate peroxidase: a hydrogen peroxide-scavenging enzyme in plants. Physiol. Plant, 85(2): 235–241.

Beauchamp C, Fridovich I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal., Biochem., 44(1): 276–287.

Bowler C, Camp W V, Montagu M V, Inzé D, Asada K. 1994. Superoxide dismutase in plants. Crit. Rev. Plant. Sci., 13(3): 199–218.

Bowler C, Montagu M V, lnzé D. 1992. Superoxide dismutase and stress tolerance. Annu. Rev. Plant. Physiol. Mol. Biol., 43: 83–116.

Bradford M M, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248–254.

Chance B, Sies H, Boveris A. 1979. Hydroperoxide metabolism in mammalian organs. Physiol. Rev., 59(3): 527–605.

Costa H, Gallego S M, Tomaro M L. 2002. Effect of UV-B radiation on antioxidant defense system in sunflower cotyledons. Plant Sci., 162(6): 939–945.

Dias M A, Costa M M. 1983. Effect of low salt concentrations on nitrate reductase and peroxidase of sugar beet leaves. J. Exp. Bot., 34(5): 537–543.

Diffey B L. 1991. Solar ultraviolet radiation effects on biological systems. Phys. Med. Biol., 36(3): 299–328.

Dummermuth A L, Karsten U, Fisch K M, König G M, Wiencke C. 2003. Responses of marine macroalgae to hydrogenperoxide stress. J. E xp. Mar. Bio l. Eco l., 22(1): 103–121.

Franklin L, Forster R. 1997. The changing irradiance environment: consequences for marine macrophyte physiology, productivity and ecology. Eur. J. Phycol., 32(3): 207–232.

Giannopolitis C N, Ries S K. 1977. Superoxide dismutases: I. Occurrence in higher plants. Plant. Physiol., 59(2): 309–314.

Guillard R R L, Ryther J H. 1962. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (Cleve) Gran. Can. J. Microbiol., 8(2): 229–239.

Halliwell B. 1987. Oxidative damage, lipid peroxidation and antioxidant protection in chloroplasts. Chem. Phys. Lipids, 44(2–4): 327–340.

Hansen J E. 1980. Physiological considerations in the mariculture of red algae. In: Abbott I A, Foster M S, Ekund L F eds. Pacific Seaweed Aquaculture. Publications of the California Sea Grant Program, La Jolla. p.80–91.

Health R L, Packer G. 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archi. Biochem. Biophys, 125(1): 189–198.

Jordan B R. 1996. The effects of ultraviolet-B radiation on plants: a molecular perspective. Adv. Bot. Res., 22: 97–162.

Kawano T. 2003. Roles of the reactive oxygen species-generating peroxidase reactions in plant defense and growth induction. Plant Cell Rep., 21(9): 829–837.

Kirchhoff V W J H, Zamorano F, Casiccia C. 1997. UV-B enhancements at Punta Arenas, Chile. J. Photochem. Photobiol. B: Biol., 38(2–3): 174–177.

Laemmli U K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680–685.

Larkum A W D, Wood W F. 1993. The effect of UV-B radiation on photosynthesis and respiration of phytoplankton, benthic macroalgae and seagrasses. Photosynth. Res., 36(1): 17–23.

Lee M Y, Shin H W. 2003. Cadmium-induced changes in antioxidant enzymes from the marine alga Nannochloropsis oculata. J. Appl. Phycol., 15(1): 13–19.

Lee T M, Shiu C T. 2009. Implications of mycosporine-like amino acid and antioxidant defenses in UV-B radiation tolerance for the algae species Ptercladiella capillacea and Gelidium amansii. Mar. Env iron. Res., 67(1): 8–16.

Li L X, Zhao J Q, Tang X X. 2010. Ultraviolet irradiation induced oxidative stress and response of antioxidant system in an intertidal macroalgae Corallina officinalis L. J. Env iron. Sci., 22(5): 716–722.

Lohrmann N L, Logan B A, Johnson A S. 2004. Seasonal acclimatization of antioxidants and photosynthesis in Chondrus crispus and Mastocarpus stellatus, two cooccurring red algae with differing stress tolerances. Biol. Bull., 207(3): 225–232.

Malanga G, Puntarulo S. 1995. Oxidative stress and antioxidant content in Chlorella vulgaris after exposure to ultraviolet-B radiation. Physiol. Plant., 94(4): 672–679.

Michiels C, Raes M, Toussaint O, Remacle J. 1994. Importance of Se-glutathione peroxidase, catalase, and Cu/Zn-SOD for cell survival against oxidative stress. Free. Radic. Biol. Med., 17(3): 235–248.

Miller G, Suzuki N, Ciftci-Yilmaz S, Mittler R. 2010. Reactive oxygen species homeostasis and signalling during drought and salinity stresses. Plant Cell Environ., 33: 453–467.

Mittler R, Zilinskas B A. 1993. Detection of ascorbate peroxidase activity in native gels by inhibition of the ascorbate-dependent reduction of nitroblue tetrazolium. Anal. Biochem., 212(2): 540–546.

Mittler R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci., 7(9): 405–410.

Nakano Y, Asada K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant & Cell Physiol., 22(5): 867–880.

Nedeva D, Pouneva I, Toncheva-Panova T. 2008. Profiles of antioxidant isoenzymes in the pathosystem scenedesmus — phlyctidium after treatment with plant growth regulators. Gen. Appl. Plant Physiol., 34(3–4): 361–376.

Okamoto O K, Pinto E, Latorre L R, Bechara E J H, Colepicolo P. 2001. Antioxidant modulation in response to metalinduced oxidative stress in algal chloroplasts. Arch Environ. Contam. Toxicol., 40(1): 18–24.

Rao M V, Paliyath G, Ormrod D P. 1996. Ultraviolet-B- and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physiol., 110: 125–136.

Rautenberger R, Wiencke C, Bischof K. 2013. Acclimation to UV radiation and antioxidative defence in the endemic Antarctic brown macroalga Desmarestia anceps along a depth gradient. Polar Biol., 36: 1 779–1 789.

Rijstenbil J W. 2002. Assessment of oxidative stress in the planktonic diatom Thalassiosira pseudonana in response to UVA and UVB radiation. J. Plankton Res., 24(12): 1 277–1 288.

Roleda M Y, Wiencke C, Hanelt D, Bischof K. 2007. Sensitivity of the early life stages of macroalgae from the northern hemisphere to ultraviolet radiation. Photochem. and Photobio l., 83: 851–862.

Roleda M Y. 2009. Photosynthetic response of Arctic kelp zoospores exposed to radiation and thermal stress. Photochem. Photobiol. Sci., 8: 1 302–1 312.

Roleda M Y, Nyberg C D, Wulff A. 2012. UVR defense mechanisms in eurytopic and invasive Gracilaria vermiculophylla (Gracilariales, Rhodophyta). Physiol. Plant., 146(2): 205–216.

Sano S, Ueda M, Kitajima S, Takeda T, Shigeoka S, Kurano N, Miyachi S, Miyake C, Yokota A. 2001. Characterization of ascorbate peroxidases from unicellular red alga Galdieria partita. Plant Cell Physiol., 42(4): 433–440.

Schweikert K, Burritt D J. 2012. The organophosphate insecticide Coumaphos induces oxidative stress and increases antioxidant and detoxification defences in the green macroalgae Ulva pertusa. Aquat. Toxicol., 122–123: 86–92.

Shindell D T, Rind D, Lonergan P. 1998. Increased polar stratospheric ozones losses and delayed eventual recovery owing to increasing greenhouse-gas concentrations. Nature, 392: 589–592.

Shigeoka S, Ishikawa T, Tamoi M, Miyagawa Y, Takeda T, Yabuta Y, Yoshimura K. 2002. Regulation and function of ascorbate peroxidase isoenzymes. J. Exp. Bot., 53(372): 1 305–1 319.

Shiu C T, Lee T M. 2005. Ultraviolet-B-induced oxidative stress and responses of the ascorbate-glutathione cycle in a marine macroalga Ulva fasciata. J. Exp. Bot., 56(421): 2 851–2 865.

Sinha R P, Häder D P. 2002. UV-induced DNA damage and repair: a review. Photochem. Photobiol. Sci., 1: 225–236.

Taalas P, Kaurola J, Kylling A, Shindell D, Sausen R, Dameris M, Grewe V, Herman J, Damski J, Steil B. 2000. The impact of greenhouse gases and halogenated species on future solar UV radiation doses. Geophys. Res. Lett., 27(8): 1 127–1 130.

Van de Poll W H, Eggert A, Buma A G J, Breeman A M. 2001. Effects of UV-B induced DNA damage and photoinhibition on growth of temperate marine red macrophytes: habitat-related differences in UV-B tolerance. J. Phycol., 37(1): 30–38.

Van Loon L C. 1971. Tobacco polyphenoloxidases: a specific staining method indicating non-identify with peroxidases. Phytochemistry, 10: 503–507.

Wei H H, Zheng Y F. 2006. Analysis of the temporal and spatial distributions of the total ozone over China. J. Nanjing Inst. Met., 29(3): 390–395. (in Chinese with English abstract)

Wong S L, Chang J. 2000. Salinity and light effects on growth, photosynthesis, and respiration of Grateloupia filicina (Rhodophyta). Aquaculture, 182: 387–395.

Woodbury W, Spencer A K, Stahmann M A. 1971. An improved procedure using ferricyanide for detecting catalase isozymes. Anal. Biochem., 44(1): 301–305.

Xiong X Z, Wang G C. 1993. Distribution of solar ultraviolet radiation at the ground and its response to atmospheric ozone depletion in China. Sci. Atmos. Sinica, 17(5): 611–620. (in Chinese with English abstract)

Yu J, Tang X X, Zhang P Y, Tian J Y, Cai H J. 2004. Effects of CO2 enrichment on photosynthesis, lipid peroxidation and activities of antioxidative enzymes of Platymonas subcordiformis subjected to UV-B radiation stress. Acta Botanica Sinica, 46(6): 682–690.

Zacher K, Roleda M Y, Hanelt D, Wiencke C. 2007. UV effects on photosynthesis and DNA in propagules of three Antarctic seaweeds (Adenocystis utricularis, Monostroma hariotii and Porphyra endiviifolium). Planta, 225(6): 1 505–1 516.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Effect of UV-B irradiation on interspecific competition between Ulva pertusa and Grateloupia filicina

UV-B irradiation and allelopathy bySargassum thunbergiiaffects the activities of antioxidant enzymes and their isoenzymes inCorallina pilulifera

Response of Microcystis aeruginosa FACHB-905 to different nutrient ratios and changes in phosphorus chemistry

Antioxidant system responses in two co-occurring green-tide algae under stress conditions

Antioxidant and isozyme features of two strains of Laminaria japonica (Phaeophyceae)