Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas

Gang Liu; Pin Huan; Baozhong Liu

doi:10.1007/s00343-014-3360-7

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Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas

OriginalPaper | Updated：2023-05-16

- Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas
- Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas
- 海洋湖沼学报（英文） 2014年32卷第6期页码：1224-1231
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 31001102) and the National Basic Research Program of China (973 Program) (No. 2010CB126403)
- DOI：10.1007/s00343-014-3360-7
  中图分类号：
- 网络首发：2014-05-24，
  
  纸质出版：2014
- Accepted：
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Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas[J]. 海洋湖沼学报（英文）, 2014,32(6):1224-1231.

Liu, G., Huan, P. & Liu, B. Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas, ., Chin. J. Ocean. Limnol. 32, 1224–1231 (2014). https://doi.org/10.1007/s00343-014-3360-7
Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas[J]. 海洋湖沼学报（英文）, 2014,32(6):1224-1231. DOI： 10.1007/s00343-014-3360-7.

Liu, G., Huan, P. & Liu, B. Cloning and expression patterns of two Smad genes during embryonic development and shell formation of the Pacific oyster Crassostrea gigas, ., Chin. J. Ocean. Limnol. 32, 1224–1231 (2014). https://doi.org/10.1007/s00343-014-3360-7 DOI：

摘要

Abstract

Increasing evidence indicates that transforming growth factor β (TGF-β) signaling pathways play many important roles in the early development of mollusks. However

limited information is known concerning their detailed mechanisms. Here

we describe the identification

cloning and characterization of two Smad genes

the key components of TGF-β signaling pathways

from the Pacific oyster Crassostrea gigas. Sequence analysis of the two genes

designated as cgi-smad1/5/8 and cgi-smad4

revealed conserved functional characteristics. The two genes were widely expressed in embryos and larvae

suggesting multiple roles in the early development of C. gigas. The mRNA of the two genes aggregated in the D quadrant and cgi-smad4 was highly expressed on the dorsal side of the gastrula

indicating that TGF-β signaling pathways may be involved in dorsoventral patterning in C. gigas. Furthermore

high expression levels of the two genes in the shell fields of embryos at different stages suggested important roles for TGF-β signaling pathways in particular phases of shell development

including the formation of the initial shell field and the biomineralization of larval shells. The results of this study provide fundamental support for elucidating how TGF-β signaling pathways participate in the early development of bivalve mollusks

and suggest that further work is warranted to this end.

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references

Derynck R, Zhang Y E. 2003. Smad-dependent and Smad-independent pathways in TGF-β family signalling. Nature, 425(6958): 577–584.

Derynck R, Zhang Y. 1996. Intracellular signalling: the mad way to do it. Curr. Biol., 6(10): 1 226–1 229.

Fleury E, Fabioux C, Lelong C, Favrel P, Huvet A. 2008. Characterization of a gonad-specific transforming growth factor-β superfamily member differentially expressed during the reproductive cycle of the oyster Crassostrea gigas. Gene, 410(1): 187–196.

Gadue P, Huber T L, Paddison P J, Keller G M. 2006. Wnt and TGF-β signaling are required for the induction of an in vitro model of primitive streak formation using embryonic stem cells. P. Natl. Acad. Sci. USA, 103(45): 16 806–16 811.

Graff J M, Thies R S, Song J J, Celeste A J, Melton D A. 1994. Studies with a Xenopus BMP receptor suggest that ventral mesoderm-inducing signals override dorsal signals in vivo. Cell, 79(1): 169–179.

Hogan B L M. 1996b. Bone morphogenetic proteins in development. Current Opinion in Genetics & Development, 6(4): 432–438.

Hogan B. 1996a. Bone morphogenetic proteins: multifunctional regulators of vertebrate development. Gene Dev., 10(13): 1 580–1 594.

Huan P, Wang H, Dong B, Liu B. 2012. Identification of differentially expressed proteins involved in the early larval development of the Pacific oyster Crassostrea gigas. J. Proteomics, 75(13): 3 855–3 865.

Huvet A, Fleury E, Corporeau C, Quillien V, Daniel J, Riviere G, Boudry P, Fabioux C. 2012. In vivo RNA interference of a gonad-specific transforming growth factor-β in the Pacific oyster Crassostrea gigas. Mar. Biotechnol., 14(4): 402–410.

Iijima M, Takeuchi T, Sarashina I, Endo K. 2008. Expression patterns of engrailed and dpp in the gastropod Lymnaea stagnalis. Dev. Genes Evol., 218(5): 237–251.

Kin K, Kakoi S, Wada H. 2009. A novel role for dpp in the shaping of bivalve shells revealed in a conserved molluscan developmental program. Dev. Biol., 329(1): 152–166.

Kishigami S, Mishina Y. 2005. BMP signaling and early embryonic patterning. Cytokine. Growth F. R., 16(3): 265–278.

Kobayashi A, Sasakura Y, Ogasawara M, Makabe K W. 1999. A maternal RNA encoding smad1/5 is segregated to animal blastomeres during ascidian development. Dev. Growth Differ., 41(4): 419–427.

Marchler-Bauer A, Anderson J B, Chitsaz F, Derbyshire M K, DeWeese-Scott C, Fong J H, Geer L Y, Geer R C, Gonzales N R, Gwadz M. 2009. CDD: specific functional annotation with the Conserved Domain Database. Nucleic Acids Res., 37(Suppl. 1): D205–D210.

Massagué J, Seoane J, Wotton D. 2005. Smad transcription factors. Gene Dev., 19(23): 2 783–2 810.

Massagué J, Wotton D. 2000. Transcriptional control by the TGF-β/Smad signaling system. EMBO J., 19(8): 1 745.

Maures T J, Duan C. 2002. Structure, developmental expression, and physiological regulation of zebrafish IGF binding protein-1. Endocrinology, 143(7): 2 722–2 731.

Nakayama T, Cui Y, Christian J. 2000. Regulation of BMP/Dpp signaling during embryonic development. Cell. Mol. Life Sci., 57(6): 943–956.

Nederbragt A J, van Loon A E, Dictus W J A G. 2002. Expression of Patella vulgata orthologs of engrailed and dpp-BMP2 / 4 in adjacent domains during molluscan shell development suggests a conserved compartment boundary mechanism. Dev. Biol., 246(2): 341–355.

Raftery L, Sutherland D J. 1999. TGF-β family signal transduction in Drosophila development: from Mad to Smads. Dev. Biol., 210(2): 251–268.

Shi Y. 2001. Structural insights on Smad function in TGFβ signaling. Bioessays, 23(3): 223–232.

Shimizu K, Sarashina I, Kagi H, Endo K. 2011. Possible functions of Dpp in gastropod shell formation and shell coiling. Dev. Genes Evol., 221(2): 59–68.

Wang X, Liu B, Xiang J. 2009. Cloning, characterization and expression of ferritin subunit from clam Meretrix meretrix in different larval stages. Comp. Biochem. Physiol. B — Biochem. Mol., 154(1): 12–16.

Winnier G, Blessing M, Labosky P A, Hogan B. 1995. Bone morphogenetic protein-4 is required for mesoderm formation and patterning in the mouse. Gene Dev., 9(17): 2 105–2 116.

Zhang G, Fang X, Guo X, Li L, Luo R, Xu F, Yang P, Zhang L, Wang X, Qi H. 2012. The oyster genome reveals stress adaptation and complexity of shell formation. Nature, 490: 49–54.

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