White spot syndrome virus inactivation study by using gamma irradiation

Marzieh Heidareh; Farahnaz Motamedi Sedeh; Mehdi Soltani; Saeed Rajabifar; Mohammad Afsharnasab; Aghil Dashtiannasab

doi:10.1007/s00343-014-3333-x

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White spot syndrome virus inactivation study by using gamma irradiation

OriginalPaper | Updated：2023-05-16

- White spot syndrome virus inactivation study by using gamma irradiation
- White spot syndrome virus inactivation study by using gamma irradiation
- 海洋湖沼学报（英文） 2014年32卷第5期页码：1024-1028
- Affiliations：
  
  1. Nuclear Science and Technology Research Institute
  2. Veterinary Faculty University of Tehran
  3. Iran Fisheries Research Institute
- Author bio：
- Funds：
  
  Supported by the Nuclear Science and Technology Research Institute, Karaj, Iran (No. A87A061, 2009)
- DOI：10.1007/s00343-014-3333-x
  中图分类号：
- 网络首发：2014-07-02，
  
  纸质出版：2014
- Accepted：
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White spot syndrome virus inactivation study by using gamma irradiation[J]. 海洋湖沼学报（英文）, 2014,32(5):1024-1028.

Heidareh, M., Sedeh, F.M., Soltani, M. et al. White spot syndrome virus inactivation study by using gamma irradiation., Chin. J. Ocean. Limnol. 32, 1024–1028 (2014). https://doi.org/10.1007/s00343-014-3333-x
White spot syndrome virus inactivation study by using gamma irradiation[J]. 海洋湖沼学报（英文）, 2014,32(5):1024-1028. DOI： 10.1007/s00343-014-3333-x.

Heidareh, M., Sedeh, F.M., Soltani, M. et al. White spot syndrome virus inactivation study by using gamma irradiation., Chin. J. Ocean. Limnol. 32, 1024–1028 (2014). https://doi.org/10.1007/s00343-014-3333-x DOI：

摘要

Abstract

The present study was conducted to investigate the effect of gamma irradiation on white spot syndrome virus (WSSV). White spot syndrome virus is a pathogen of major economic importance in cultured penaeid shrimp industries. White spot disease can cause mortalities reaching 100% within 3–10 days of gross signs appearing. During the period of culture

immunostimulant agents and vaccines may provide potential methods to protect shrimps from opportunistic and pathogenic microrganisms. In this study

firstly

WSSV was isolated from infected shrimp and then multiplied in crayfish. WSSV was purified from the infected crayfish haemolymph by sucrose gradient and confirmed by transmission electron microscopy. In vivo virus titration was performed in shrimp

Penaeus semisulcatus. The LD50 of live virus stock was calculated 10 5.4/mL. Shrimp post-larvae (1–2 g) were treated with gamma-irradiated (different doses) WSSV (100 to 10−4 dilutions) for a period of 10 days. The dose/survival curve for irradiated and un-irradiated WSSV was drawn; the optimum dose range for inactivation of WSSV and unaltered antigenicity was obtained 14–15 kGy. This preliminary information suggests that shrimp appear to benefit from treatment with gammairradiated WSSV especially at 14–15 KGy.

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references

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