Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique

Wei Zhou; Hongye Ding; Zhenghong Sui; Zhongxia Wang; Jinguo Wang

doi:10.1007/s00343-014-3209-0

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Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique

OriginalPaper | Updated：2023-05-16

- Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique
- Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique
- 海洋湖沼学报（英文） 2014年32卷第3期页码：522-526
- Affiliations：
  
  Key Laboratory of Marine Genetics and Breeding of Ministry of Education Ocean University of China
- Author bio：
- Funds：
  
  Supported by the “Twelfth Five-Year-Plan” of National Science and Technology for the Rural Development in China (No. 2012AA10A411) and the Public Welfare Project of the Ministry of Agriculture of China (No. 200903030)
- DOI：10.1007/s00343-014-3209-0
  中图分类号：
- 网络首发：2014-05-04，
  
  纸质出版：2014
- Accepted：
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Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique[J]. 海洋湖沼学报（英文）, 2014,32(3):522-526.

Zhou, W., Ding, H., Sui, Z. et al. Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique., Chin. J. Ocean. Limnol. 32, 522–526 (2014). https://doi.org/10.1007/s00343-014-3209-0
Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique[J]. 海洋湖沼学报（英文）, 2014,32(3):522-526. DOI： 10.1007/s00343-014-3209-0.

Zhou, W., Ding, H., Sui, Z. et al. Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique., Chin. J. Ocean. Limnol. 32, 522–526 (2014). https://doi.org/10.1007/s00343-014-3209-0 DOI：

摘要

Abstract

The red alga Gracilariopsis lemaneiformis (Bory) is an economically valuable macroalgae. As a means to identify the sex of immature Gracilariopsis lemaneiformis

the amplified fragment length polymorphism (AFLP) technique was used to search for possible sex- or phase-related markers in male gametophytes

female gametophytes

and tetrasporophytes

respectively. Seven AFLP selective amplification primers were used in this study. The primer combination E-TG/M-CCA detected a specific band linked to male gametophytes. The DNA fragment was recovered and a 402-bp fragment was sequenced. However

no DNA sequence match was found in public databases. Sequence characterized amplified region (SCAR) primers were designed from the sequence to test the repeatability of the relationship to the sex

using 69 male gametophytes

139 female gametophytes

and 47 tetrasporophytes. The test results demonstrate a good linkage and repeatability of the SCAR marker to sex. The SCAR primers developed in this study could reduce the time required for sex identification of Gracilariopsis lemaneiformis by four to six months. This can reduce both the time investment and number of specimens required in breeding experiments.

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references

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