Transcriptomic analysis of Synechocystis sp. PCC6803 under low-temperature stress

Zhixiang Liu; Hongli Cui; Zhengyi Liu; Yinchu Wang; Yulin Cui; Zhaopu Liu; Song Qin

doi:10.1007/s00343-014-3174-7

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Transcriptomic analysis of Synechocystis sp. PCC6803 under low-temperature stress

OriginalPaper | Updated：2023-05-16

- Transcriptomic analysis of Synechocystis sp. PCC6803 under low-temperature stress
- Transcriptomic analysis of Synechocystis sp. PCC6803 under low-temperature stress
- 海洋湖沼学报（英文） 2014年32卷第2期页码：403-418
- Affiliations：
  
  1. College of Resources and Environmental Sciences Key Laboratory of Marine Biology Nanjing Agricultural University
  2. Yantai Institute of Coastal Zone Research Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 40876082), the International Innovation Partnership Program: Typical Environmental Process and Effects on Resources in Coastal Zone Area, the Public Science and Technology Research Funds Projects of the Ocean (Nos. 200905021, 201205027), the Outstanding Young Scholars Fellowship of Shandong Province (Molecular Phycology, No. JQ200914), the Natural Science Foundation of Shandong Province (No. ZR2012DQ015), and the Guangdong Province Comprehensive Strategic Cooperation Project of the Chinese Academy of Sciences (No. 2011A090100040)
- DOI：10.1007/s00343-014-3174-7
  中图分类号：
- 网络首发：2014-03-06，
  
  纸质出版：2014
- Accepted：
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Transcriptomic analysis of Synechocystis sp. PCC6803 under low-temperature stress[J]. 海洋湖沼学报（英文）, 2014,32(2):403-418.

Liu, Z., Cui, H., Liu, Z. et al. Transcriptomic analysis of Synechocystis sp. PCC6803 under low-temperature stress., Chin. J. Ocean. Limnol. 32, 403–418 (2014). https://doi.org/10.1007/s00343-014-3174-7
Transcriptomic analysis of Synechocystis sp. PCC6803 under low-temperature stress[J]. 海洋湖沼学报（英文）, 2014,32(2):403-418. DOI： 10.1007/s00343-014-3174-7.

Liu, Z., Cui, H., Liu, Z. et al. Transcriptomic analysis of Synechocystis sp. PCC6803 under low-temperature stress., Chin. J. Ocean. Limnol. 32, 403–418 (2014). https://doi.org/10.1007/s00343-014-3174-7 DOI：

摘要

Abstract

In this study

cDNA microarrays were developed from 3569 mRNA reads to analyze the expression profiles of the transcriptomes of Synechocystis sp. PCC6803 under low temperature (LT) stress. Among the genes on the cDNA microarrays

899 LT-affected genes exhibited a 1.5-fold (or greater) difference in expression compared with the genes from normal unstressed Synechocystis sp. PCC6803. Of the differentially expressed genes

353 were up-regulated and 246 were down-regulated. The results showed that genes involved in photosynthesis were activated at LT (10°C)

including genes for photosystem I

photosystem II

photosynthetic electron transport

and cytochrome b6/f complex. Moreover

desB

one of four genes that encode the fatty acid desaturases

was also induced by LT. However

the LT conditions to some degree enhanced the transcription of some genes. In addition

LT (10°C) may reduce cellular motility by regulating the transcription of spkA (sll1575)

a serine/threonine protein kinase. The results reported in this study may contribute to a better understanding of the responses of the Synechocystis cell to LT

including pathways involved in photosynthesis and repair.

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Keywords

references

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