Vitreoscilla hemoglobin gene (vgb) improves lutein production in Chlorella vulgaris

Ruijuan Ma; Xiangzhi Lin

doi:10.1007/s00343-014-3142-2

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Vitreoscilla hemoglobin gene (vgb) improves lutein production in Chlorella vulgaris

OriginalPaper | Updated：2023-05-16

- Vitreoscilla hemoglobin gene (vgb) improves lutein production in Chlorella vulgaris
- Vitreoscilla hemoglobin gene (vgb) improves lutein production in Chlorella vulgaris
- 海洋湖沼学报（英文） 2014年32卷第2期页码：390-396
- Affiliations：
  
  Engineering Research Center of Marine Biological Resource Comprehensive Utilization Third Institute of Oceanography State Oceanic Administration
- Author bio：
- Funds：
  
  Supported by the Ocean Public Welfare Scientific Research Special Appropriation Project (No. 201005020)
- DOI：10.1007/s00343-014-3142-2
  中图分类号：
- 网络首发：2014-03-06，
  
  纸质出版：2014
- Accepted：
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Vitreoscilla hemoglobin gene (vgb) improves lutein production in Chlorella vulgaris[J]. 海洋湖沼学报（英文）, 2014,32(2):390-396.

Ma, R., Lin, X. , Vitreoscilla hemoglobin gene (vgb) improves lutein production in Chlorella vulgaris, ., Chin. J. Ocean. Limnol. 32, 390–396 (2014). https://doi.org/10.1007/s00343-014-3142-2
Vitreoscilla hemoglobin gene (vgb) improves lutein production in Chlorella vulgaris[J]. 海洋湖沼学报（英文）, 2014,32(2):390-396. DOI： 10.1007/s00343-014-3142-2.

Ma, R., Lin, X. , Vitreoscilla hemoglobin gene (vgb) improves lutein production in Chlorella vulgaris, ., Chin. J. Ocean. Limnol. 32, 390–396 (2014). https://doi.org/10.1007/s00343-014-3142-2 DOI：

摘要

Abstract

Vitreoscilla hemoglobin is an oxygen-binding protein that promotes oxygen delivery and reduces oxygen consumption under low oxygen conditions to increase the efficiency of cell respiration and metabolism. In this study

we introduced a Vitreoscilla hemoglobin gene (vgb) into Chlorella vulgaris by Agrobacterium tumefaciens -mediated transformation (ATMT). PCR analysis confirmed that the vgb gene was successfully integrated into the Chlorella vulgaris genome. Analysis of biomass obtained in shake flasks revealed transformant biomass concentrations as high as 3.28 g/L

which was 38.81% higher than that of the wild-type strain. Lutein content of transformants also increased slightly. Further experiments recovered a maximum lutein yield of 2.91 mg/L from the transformants

which was 36.77% higher than that of the wild-type strain. The above results suggest that integrated expression of the vgb gene may improve cell growth and lutein yield in Chlorella vulgaris

with applications to lutein production from Chlorella during fermentation.

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references

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