Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta)

Zhanru Shao; Fuli Liu; Qiuying Li; Jianting Yao; Delin Duan

doi:10.1007/s00343-014-3130-6

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Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta)

OriginalPaper | Updated：2023-05-16

- Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta)
- Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta)
- 海洋湖沼学报（英文） 2014年32卷第2期页码：377-389
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
  3. Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
- Author bio：
- Funds：
  
  Supported by the Agriculture Science & Technology Achievements Transformation Fund (No. 2011GB24910005), the Modern Agricultural-Industry Technology Research Project (No. 200903030), the National High Technology Research and Development Program of China (863 Program) (No. 2012AA10A406), the Shandong Agriculture Breeding Engineering Biological Resources Innovation of Research Project, and the National “Twelfth Five-Year” Plan for Science & Technology Support (No. 2013BAB01B01);SHAO Zhanru and LIU Fuli contributed equally to this work.
- DOI：10.1007/s00343-014-3130-6
  中图分类号：
- 网络首发：2013-12-28，
  
  纸质出版：2014
- Accepted：
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Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta)[J]. 海洋湖沼学报（英文）, 2014,32(2):377-389.

Shao, Z., Liu, F., Li, Q. et al. Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta)., Chin. J. Ocean. Limnol. 32, 377–389 (2014). https://doi.org/10.1007/s00343-014-3130-6
Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta)[J]. 海洋湖沼学报（英文）, 2014,32(2):377-389. DOI： 10.1007/s00343-014-3130-6.

Shao, Z., Liu, F., Li, Q. et al. Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta)., Chin. J. Ocean. Limnol. 32, 377–389 (2014). https://doi.org/10.1007/s00343-014-3130-6 DOI：

摘要

Abstract

Saccharina japonica is a common macroalga in sublittoral communities of cold seawater environments

and consequently may have highly efficient ribulose-1

5-bisphosphate carboxylase/oxygenase (Rubisco) activity for carbon assimilation. In our study

we cloned the full-length Rubisco gene from S. japonica (SJ-rbc). It contained an open reading frame for a large subunit gene (SJ — rbcL) of 1 467 bp

a small subunit gene (SJ-rbcS) of 420 bp

and a SJ-rbcL/S intergenic spacer of 269 bp. The deduced peptides of SJ-rbcL and SJ-rbcS were 488 and 139 amino acids with theoretical molecular weights and isoelectric points of 53.97 kDa

5.81 and 15.84 kDa

4.71

respectively. After induction with 1 mmol/L isopropyl-β-D-thiogalactopyranoside for 5 h and purification by Ni2+ affinity chromatography

electrophoresis and western blot detection demonstrated successful expression of the 55 kDa SJ-rbcL protein. Real-time quantitative PCR showed that the mRNA levels of SJ-rbcL in gametophytes increased when transferred into normal growth conditions and exhibited diurnal variations: increased expression during the day but suppressed expression at night. This observation implied that Rubisco played a role in normal gametophytic growth and development. In juvenile sporophytes

mRNA levels of SJ-rbcL

carbonic anhydrase

Calvin-Benson-Bassham cycle-related enzyme

and chloroplast light-harvesting protein were remarkably increased under continuous light irradiance. Similarly

expression of these genes was up-regulated under blue light irradiance at 350 μmol/(m2·s). Our results indicate that long-term white light and short-term blue light irradiance enhances juvenile sporophytic growth by synergistic effects of various photosynthetic elements.

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