A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis

Jiafeng Wang; Fei Xu; Li Li; Guofan Zhang

doi:10.1007/s00343-014-3124-4

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A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis

OriginalPaper | Updated：2023-05-16

- A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis
- A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis
- 海洋湖沼学报（英文） 2014年32卷第2期页码：419-425
- Affiliations：
  
  1. National & Local Joint Engineering Laboratory of Ecological Mariculture Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Basic Research Program of China (973 Program) (No. 2010CB126402), the National Natural Science Foundation of China (Nos. 40730845, 41206149), the Shandong Provincial Natural Science Foundation (No. ZR2010DQ024), the National High Technology Research and Development Program of China (863 Program) (No. 2012AA10A405), the Earmarked Fund for Modern Agro-Industry Technology Research System (No. CARS-48), the Taishan Scholar Program of Shandong Province, and the Taishan Scholar Climbing Program of Shandong Province
- DOI：10.1007/s00343-014-3124-4
  中图分类号：
- 网络首发：2014-03-06，
  
  纸质出版：2014
- Accepted：
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A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis[J]. 海洋湖沼学报（英文）, 2014,32(2):419-425.

Wang, J., Xu, F., Li, L. et al. A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis., Chin. J. Ocean. Limnol. 32, 419–425 (2014). https://doi.org/10.1007/s00343-014-3124-4
A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis[J]. 海洋湖沼学报（英文）, 2014,32(2):419-425. DOI： 10.1007/s00343-014-3124-4.

Wang, J., Xu, F., Li, L. et al. A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis., Chin. J. Ocean. Limnol. 32, 419–425 (2014). https://doi.org/10.1007/s00343-014-3124-4 DOI：

摘要

Abstract

The high phenotypic plasticity in the shell of oysters presents a challenge during taxonomic and phylogenetic studies of these economically important bivalves. However

because DNA can exhibit marked differences among morphologically similar species

DNA barcoding offers a potential means for oyster identification. We analyzed the complete sequences of the cytochrome oxidase subunit I (COI) of five common Crassostrea species in China (including Hong Kong oyster C. hongkongensis

Jinjiang oyster C. ariakensis

Portuguese oyster C. angulata

Kumamoto oyster C. sikamea

and Pacific oyster C. gigas) and screened for distinct fragments. Using these distinct fragments on a high-resolution melting analysis platform

we developed an identification method that does not rely on species-specific PCR or fragment length polymorphism and is efficient

reliable

and easy to visualize. Using a single pair of primers (Oyster-COI-1)

we were able to successfully distinguish among the five oyster species. This new method provides a simple and powerful tool for the identification of oyster species.

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references

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