Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations

Liping You; Xuanxuan Ning; Leilei Chen; Linbao Zhang; Jianmin Zhao; Xiaoli Liu; Huifeng Wu

doi:10.1007/s00343-014-3111-9

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Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations

OriginalPaper | Updated：2023-05-16

- Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations
- Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations
- 海洋湖沼学报（英文） 2014年32卷第2期页码：334-343
- Affiliations：
  
  1. Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation Yantai Institute of Coastal Zone Research Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
  3. Yantai Oceanic Environmental Monitoring Central Station State Oceanic Administration
- Author bio：
- Funds：
  
  Supported by the 100 Talents Program of the Chinese Academy of Sciences, the National Natural Science Foundation of China (No. 41206105), and the Key Deployment Program of Chinese Academy of Sciences (No. KZZD-EW-14-03)
- DOI：10.1007/s00343-014-3111-9
  中图分类号：
- 网络首发：2014-03-06，
  
  纸质出版：2014
- Accepted：
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Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations[J]. 海洋湖沼学报（英文）, 2014,32(2):334-343.

You, L., Ning, X., Chen, L. et al. Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations., Chin. J. Ocean. Limnol. 32, 334–343 (2014). https://doi.org/10.1007/s00343-014-3111-9
Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations[J]. 海洋湖沼学报（英文）, 2014,32(2):334-343. DOI： 10.1007/s00343-014-3111-9.

You, L., Ning, X., Chen, L. et al. Expression profiles of two small heat shock proteins and antioxidant enzyme activity in Mytilus galloprovincialis exposed to cadmium at environmentally relevant concentrations., Chin. J. Ocean. Limnol. 32, 334–343 (2014). https://doi.org/10.1007/s00343-014-3111-9 DOI：

摘要

Abstract

Small heat shock proteins encompass a widespread but diverse class of proteins

which play key roles in protecting organisms from various stressors. In the present study

the full-length cDNAs of two small heat shock proteins (MgsHSP22 and MgsHSP24.1) were cloned from Mytilus galloprovincialis

which encoded peptides of 181 and 247 amino acids

respectively. Both MgsHSP22 and MgsHSP24.1 were detected in all tissues examined by real-time PCR

with the highest expression being observed in muscle and gonad tissues. The real-time PCR results revealed that Cd significantly inhibited MgsHSP22 expression at 24 h and MgsHSP24.1 at 24 and 48 h under 5 μg/L Cd 2+ exposure. MgsHSP24.1 expression was also significantly inhibited after 50 μg/L Cd2+ exposure for 48 h. With regard to antioxidant enzymes

increased GPx and CAT activity were detected under Cd2+ stress (5 and 50 μg/L)

while no significant difference in SOD activity was observed throughout the experiment. Overall

both MgsHsps and antioxidant enzymes revealed their potential as Cd stress biomarkers in M. galloprovincialis.

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references

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