Effect of Fe3+ on the growth and lipid content of Isochrysis galbana

Zhiyuan Liu; Guangce Wang

doi:10.1007/s00343-014-3110-x

Your Location：

Home >

Browse articles >

Effect of Fe3+ on the growth and lipid content of Isochrysis galbana

OriginalPaper | Updated：2023-05-16

- Effect of Fe3+ on the growth and lipid content of Isochrysis galbana
- Effect of Fe3+ on the growth and lipid content of Isochrysis galbana
- 海洋湖沼学报（英文） 2014年32卷第1期页码：47-53
- Affiliations：
  
  1. College of Oceanology Hainan University
  2. Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 40966002) and the Natural Science Foundation of Hainan Province, China (No. 409001)
- DOI：10.1007/s00343-014-3110-x
  中图分类号：
- 网络首发：2014-02-21，
  
  纸质出版：2014
- Accepted：
Scan QR Code
Effect of Fe3+ on the growth and lipid content of Isochrysis galbana[J]. 海洋湖沼学报（英文）, 2014,32(1):47-53.

Liu, Z., Wang, G. Effect of Fe3+ on the growth and lipid content of Isochrysis galbana, ., Chin. J. Ocean. Limnol. 32, 47–53 (2014). https://doi.org/10.1007/s00343-014-3110-x
Effect of Fe3+ on the growth and lipid content of Isochrysis galbana[J]. 海洋湖沼学报（英文）, 2014,32(1):47-53. DOI： 10.1007/s00343-014-3110-x.

Liu, Z., Wang, G. Effect of Fe3+ on the growth and lipid content of Isochrysis galbana, ., Chin. J. Ocean. Limnol. 32, 47–53 (2014). https://doi.org/10.1007/s00343-014-3110-x DOI：

摘要

Abstract

Inducing lipid accumulation in microalgae cells without suppressing cell growth is vital to the economical production of biodiesel from microalgae. In two experiments

we demonstrate that the cell concentration and lipid content of marine microalgae Isochrysis galbana depend upon the iron concentration in the growth media. In Experiment I

adding chelated FeCl3 to the medium at the late exponential growth phase prolonged this phase and increased the lipid content in I. galbana cells. The final cell density and lipid content of I. galbana supplemented with chelated FeCl3 was approximately 2 and 1.65 times higher than that of non-supplemented cultures

respectively. In Experiment II

I. galbana cells in the late exponential phase were collected and re-inoculated into new media containing Fe3+ at various concentrations. The final cell concentration and lipid content were maximized at the highest iron concentration (38% biomass by dry weight at 1.2×10−5 mol/L Fe3+). In this study

intracellular neutral lipid storage was evaluated by fluorescent spectrophotometry using fluorochrome Nile red

and the measurement conditions were optimized.

关键词

Keywords

references

Alonzo F, Mayzaud P. 1999. Spectrofluorometric quantification of neutral and polar lipids in zooplankton using Nile red. Mar. Chem., 67: 289–301.

Bligh E G, Dyer W J. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol., 37: 911–917.

Boyd P W, Watson A J, Law C S, Abraham E R, Trull T, Murdoch R, Bakker D C E, Bowie A R, Buesseler K O, Chang H, Charette M, Croot P, Downing K, Frew R, Gall M, Hadfield M, Hall J, Harvey M, Jameson G, LaRoche J, Liddicoat M, Ling R, Maldonado M T, McKay R M, Nodder S, Pickmere S, Pridmore R, Rintoul S, Safi K, Sutton P, Strzepek R, Tanneberger K, Turner S, Waite A, Zeldis J. 2000. A mesoscale phytoplankton bloom in the polar Southern Ocean stimulated by iron fertilization. Nature, 407: 695–702.

Brown M R, Dunstan G A, Jeffrey S W, Volkman J K, Barrett S M, LeRoi J M. 1993. The influence of irradiance on the biochemical composition of the Prymnesiophyte Isochrysis sp. J. Phycol., 29: 601–612.

Chen W, Sommerfeld M, Hu Q. 2011. Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae. Bioresour. Technol., 102: 135–141.

Chisti Y. 2007. Biodiesel from microalgae. Biotechnol. Adv., 25: 294–306.

Cooksey K E, Guckert J B, Williams S A, Collis P R. 1987. Fluorometric determination of the neutral lipid content of microalgal cells using Nile Red. J. Microbiol. Meth., 6: 333–345.

de la Jara A, Mendoza H, Martel A et al. 2003. Flow cytometric determination of lipid content in a marine dinoflagellate Crypthecodinium cohnii. J. Appl. Phycol., 15: 433–438.

Elsey D, Jameson D, Raleigh B, Cooney M J. 2007. Fluorescent measurement of microalgal neutral lipids. J. Appl. Phycol., 68: 639–642.

Floreto E A T, Teshima S, Ishikawa M. 1996. Effects of nitrogen and phosphorus on the growth and fatty acid composition of Ulva pertusa Kjellman (Chlorophyta). Bot. Mar., 39: 69–74.

Glover H. 1977. Effects of iron deficiency on Isochrysis galbana (Chrysophyceae) and Phaeodactylum tricornutum (Bacillariophyceae). J. Phycol., 13: 208–212.

Golterman H L. 1969. Methods for Chemical Analysis of Fresh Waters. International Biological Programme Handbook. No.8. Oxford: Bladkwell Scientific Publications.

Greenspan P, Mayer E P, Fowle S D. 1985. Nile red: a selective fluorescent stain for intracellular lipid droplets. J. Cell Biol., 100: 965–973.

Guillard R R, Ryther J H. 1962. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea (Cleve). Gran. Can. J. Microbiol., 8: 229–239.

Hutchins D A, Bruland K W. 1998. Iron-limited diatom growth and Si:N uptake ratios in a coastal upwelling regime. Nature, 393: 561–564.

Illman A M, Scragg A H, Shales S W. 2000. Increase in Chlorella strains calorific values when grown in low nitrogen medium. Enzyme Microb. Tech., 27: 631–635.

Khozin-Goldberg I, Cohen Z. 2006. The effect of phosphate starvation on the lipid and fatty acid composition of the fresh water eustigmatophyte Monodus subterraneus. Phytochemistry, 67: 696–701.

Kimura K, Yamaoka M, Kamisaka Y. 2004. Rapid estimation of lipids in oleaginous fungi and yeasts using Nile red fluorescence. J. Microbiol. Meth., 56: 331–338.

Lee SJ, Yoon B-D, Oh H-M. 1998. Rapid method for the determination of lipid from the green alga Botryococcus braunii. Biotechnol Tech., 12: 553–556.

Lin Y, Harrison P J. 2000. Research on red tide occurrences using enclosed experimental ecosystems in west Xiamen Harbor, China-relationship between various factors and red tide occurrences. Chin. J. Oceanol. Limnol., 18: 148–156.

Liu Z Y, Wang G C, Zhou B C. 2008. Effect of iron on growth and lipid accumulation in Chlorella vulgaris. Bioresour. Technol., 99: 4 717–4 722.

Lombardi A T, Wangersky P J. 1991. Influence of phosporus and silicon on lipid class production by the marine diatom Chaetoceros gracilis grown in turbidostat cage cultures. Mar. Eco. Progr. Ser., 77: 39–47.

Lynn S G, Kilham S S, Kreeger D A, Interlandi S J. 2000. Effect of nutrient availability on the biochemical and elemental stoichiometry in freshwater diatom Stephanodiscus minutulus acillariophyceae. J. Phycol., 36: 510–522.

McGinnis K M, Dempster T A, Sommerfeld M R. 1997. Characterization of the growth and lipid content of the diatom Chaetoceros muelleri. J. Appl. Phycol., 9: 19–24.

Molina Grima E, García Camacho F, Sánchez Pérez J A, García Sánchez J L. 1994 Biochemical productivity and fatty acid profiles of Isochrysis galbana Parke and Teraselmis sp. as a function of incident light intensity. Process Biochem., 29: 119–126.

Rao A R, Dayananda C, Sarada R, Shamala T R, Ravishankar G A. 2007. Effect of salinity on growth of green alga Botryococcus braunii and its constituents. Process Biochem., 98: 560–564.

Reitan K I, Rainuzzo J R, Olsen Y. 1994. Effect of nutrient limitation on fatty acid and lipid content of marine microalgae. J. Phycol., 30: 972–979.

Schenk P M, Thomas-Hall S R, Stephens E, Marx U C, Mussgnug J H, Posten C, Kruse O, Hankamer B. 2008. Second generation biofuels: high-efficiency microalgae for biodiesel production. Bioenerg. Res., 1: 20–43.

Tang Q Y, Feng M G. 2007. DPS Data processing system: Experimental Design, Statistical Analysis, and Data Mining. Science Press, Beijing.

Terry N, Abadla J. 1986. Function of iron in chloroplasts. J. Plant. Nutr., 9: 609–646.

UNESCO. 1966. Determination of Photosynthetic Pigments in Seawater, Rep. COR/UNESCO WG 17, UNESCO Monogr. Oceanogr. Methodol., 1, Paris.

Wijffels R H, Barbosa M J. 2010. An outlook on microalgal biofuels. Science, 329: 796–799.

Williams W J. 1979. Handbook of Anion Determination. Butterworths, London. p.119.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Effect of carbon source on growth and lipid accumulation in Chlorella sorokiniana GXNN01

On microplastics abundance in waters of Guizhou, China

Effects of light spectra on growth and compositions of biomass, fatty acids, and pigments in three typical microalgae from different phyla

液体火箭发动机背压振荡环境下的雾化特性研究进展

2.5D编织结构复合材料温度场特征研究