Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus

Mengxin Xing; Zhanhui Hou; Yanmei Qu; Bin Liu

doi:10.1007/s00343-014-3099-1

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Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus

OriginalPaper | Updated：2023-05-16

- Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus
- Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus
- 海洋湖沼学报（英文） 2014年32卷第2期页码：316-325
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2007AA09Z444)
- DOI：10.1007/s00343-014-3099-1
  中图分类号：
- 网络首发：2014-03-06，
  
  纸质出版：2014
- Accepted：
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Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus[J]. 海洋湖沼学报（英文）, 2014,32(2):316-325.

Xing, M., Hou, Z., Qu, Y. et al. Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus, ., Chin. J. Ocean. Limnol. 32, 316–325 (2014). https://doi.org/10.1007/s00343-014-3099-1
Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus[J]. 海洋湖沼学报（英文）, 2014,32(2):316-325. DOI： 10.1007/s00343-014-3099-1.

Xing, M., Hou, Z., Qu, Y. et al. Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus, ., Chin. J. Ocean. Limnol. 32, 316–325 (2014). https://doi.org/10.1007/s00343-014-3099-1 DOI：

摘要

Abstract

Eighteen agar media were tested for the culture of gut-associated bacteria from farmed adult turbot (Scophthalmus maximus)

including 16 agar media with or without 1% gastrointestinal (GI) supernatant

or with 2% or 4% GI supernatant. A total of 1 711 colonies were analyzed and 24 operational taxonomic units (OTUs) were identified. The greatest bacterial diversity was isolated on Zobell 2216E/Zobell 2216E+ agar media

whereas MRS/MRS+ agar media produced a low diversity of colonies. Agar media with GI supernatant (1%

or 4%) showed increased diversity and yielded different profiles of OTUs from the corresponding original media

suggesting that GI supernatant provides substances that enhance the culture efficiency of bacteria from the turbot GI tract. The large majority of the colonies (82%) were γ-Proteobacteria

whereas 15.6% and 2.4% of colonies were Firmicutes and Actinobacteria

respectively. At the genus level

49.4% of all colonies were assigned to Vibrio. Other potential pathogens

including Pseudomonas

Photobacterium

and Enterobacter

and potential probiotics

including Bacillus

Paenibacillus

and Pseudomonas

were also isolated on agar media. Most cultured bacteria belonged to species that were first described in the turbot GI tract. The impact of these species on turbot physiology and health should be investigated further.

关键词

Keywords

references

Austin B. 2006. The bacterial microflora of fish, revised. Scientific World Journal, 6: 931–945.

Balcázar J L, de Blas I, Ruiz-Zarzuela I, Cunningham D, Vendrell D, Múzquiz J L. 2006. The role of probiotics in aquaculture. Vet. Microbiol., 114(3–4): 173–186.

Bruns A, Cypionka H, Overmann J. 2002. Cyclic AMP and acyl homoserine lactones increase the cultivation efficiency of heterotrophic bacteria from the central Baltic Sea. Appl. Environ. Microbiol., 68: 3 978–3 987.

Cantas L, Sørby J R, Aleström P, Sørum H. 2012. Culturable gut microbiota diversity in zebrafish. Zebrafish, 9(1): 26–37.

Cerdà-Cuéllar M, Blanch A R. 2002. Detection and identification of Vibrio scophthalmi in the intestinal microbiota of fish and evaluation of host specificity. J. Appl. Microbiol., 93(2): 261–268.

Cerdà-Cuéllar M, Rosselló-Mora R A, Lalucat J, Jofre J, Blanch A. 1997. Vibrio scophthalmi sp. Nov., a new species from turbot (Scophthalmus maximus). Int. J. Syst. Bacteriol., 47(1): 58–61.

Chabrillón M, Rico R M, Balebona M C, Moriñigo M A. 2005. Adhesion to sole, Solea senegalensis Kaup, mucus of microorganisms isolated from farmed fish, and their interaction with Photobacterium damselae subsp. piscicida. J. Fish Dis., 28: 229–237.

Chu W, Lu F, Zhu W, Kang C. 2011. Isolation and characterization of new potential probiotic bacteria based on quorum-sensing system. J. Appl. Microbiol., 110(1): 202–208.

Cole J R, Chai B, Farris R J, Wang Q, Kulam-Syed-Mohideen A S, McGarrell D M, Bandela A M, Cardenas E, Garrity G M, Tiedje J M. 2007. The ribosomal database project (RDP-II): introducing myRDP space and quality controlled public data. Nucleic Acids Res., 35: 169–172.

Connon S A, Giovannoni S J. 2002. High-throughput methods for culturing microorganisms in very-low-nutrient media yield diverse new marine isolates. Appl. Environ. Microbiol., 68(8): 3 878–3 885.

De Kievit T R, Gillis R, Marx S, Brown C, Iglewski B H. 2001. Quorum-sensing genes in Pseudomonas aeruginosa biofilms: their role and expression patterns. Appl. Environ. Microbiol., 67(4): 1 865–1 873.

De Schrijver R, Ollevier F. 2000. Protein digestion in juvenile turbot (Scophthalmus maximus) and effects of dietary administration of Vibrio proteolyticus. Aquaculture, 186: 107–116.

DeSantis T Z, Hugenholtz P, Larsen N, Rojas M, Brodie E L, Keller K, Huber T, Dalevi D, Hu P, Andersen G L. 2006. Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB. Appl. Environ. Microbiol., 72: 5 069–5 072.

Dhayanithi N B, Kumar T T, Kathiresan K. 2010. Effect of neem extract against the bacteria isolated from marine fish. J. Environ. Biol., 31(4): 409–412.

Dimitroqlou A, Merrifield D L, Carnevali O, Picchietti S, Avella M, Daniels C, Güroy D, Davies S L. 2011. Microbial manipulations to improve fish health and production-a mediterranean perspective. Fish Shellfish Immunol., 30(1): 1–16.

Fujii K, Kuwahara A, Nakamura K, Yamashita Y. 2011. Development of a simple cultivation method for isolating hitherto-uncultured cellulase-producing microbes. Appl. Microbiol. Biotechnol., 91: 1 183–1 192.

García-Aljaro C, Eberl L, Riedel K, Blanch A R. 2008. Detection of quorum-sensing-related molecules in Vibrio scophthalmi. BMC. Microbiol., 8: 138.

Gosalbes M J, Abellan J J, Durbán A, Pérez-Cobas A E, Latorre A, Moya A. 2012. Metagenomics of human microbiome: beyond 16s rDNA. Clin. Microbiol. Infect., 18(Suppl. 4): 47–49.

Gram L, Løvold T, Nielsen J, Melchiorsen J, Spanggaard B. 2001. In vitro antagonism of the probiont Pseudomonas fluorescens strain AH2 against Aeromonas salmonicida does not confer protection of salmon against furunculosis. Aquaculture, 199: 1–11.

Han S F, Liu Y C, Zhou Z G, He S X, Cao Y A, Shi P J, Yao B, Ringø E. 2010. Analysis of bacterial diversity in the intestine of grass carp (Ctenopharyngodon idellus) based on 16S rDNA gene sequences. Aquacult. Res., 42: 47–56.

Huber T, Faulkner G, Hugenholtz P. 2004. Bellerophon: a program to detect chimeric sequences in multiple sequence alignments. Bioinformatics, 20: 2 317–2 319.

Irwin J A. 2010. Extremophiles and their application to veterinary medicine. Environ. Technol., 31(8-9): 857–869.

Kaeberlein T, Lewis K, Epstein S S. 2002. Isolating ‘uncultivable’ microorganisms in pure culture in a simulated natural environment. Science, 296: 1 127–1 129.

Kennedy J, Baker P, Piper C, Cotter P D, Walsh M, Mooij M J, Bourke M B, Rea M C, O’Connor P M, Ross R P, Hill C, O’Gara F, Marchesi J R, Dobson A D. 2009. Isolation and analysis of bacteria with antimicrobial activities from the marine sponge Haliclona simulans collected from Irish waters. Mar. Biotechnol., 11(3): 384–396.

Kim B S, Kim J N, Cerniglia C E. 2011. In vitro culture conditions for maintaining a complex population of human gastrointestinal tract microbiota. J. Biomed. Biotechnol., 2011: 838 040.

Kim J J, Kim H N, Masui R, Kuramitsu S, Seo J H, Kim K, Sung M H. 2008. Isolation of uncultivable anaerobic thermophiles of the family Clostridiaceae requiring growth-supporting factors. J. Microbiol. Biotechnol., 18(4): 611–615.

Kuda T, Kyoi D, Takahashi H, Obama K, Kimura B. 2011. Detection and isolation of p-nitrophenol-lowering bacteria from intestine of marine fishes caught in Japanese waters. Mar. Pollut. Bull., 62(8): 1 622–1 627.

Lazado C C, Caipang C M A, Rajan B, Brinchmann M F, Kiron V. 2010. Characterization of GP21 and GP12: two protential probiotic bacteria isolated from the gastrointestinal tract of Atlantic cod. Probiotics & Antimicro. Prot., 2: 126–134.

Li C Q, Liu W C, Zhu P, Yang J L, Cheng K D. 2011. Phylogenetic diversity of bacteria associated with the marine sponge Gelliodes carnosa collected from the Hainan Island coastal waters of the South China Sea. Microb. Ecol., 62(4): 800–812.

Lunder T, Sørum H, Holstad G, Steigerwalt A G, Mowinckel P, Brenner D J. 2000. Phenotypic and genotypic characterization of Vibrio viscosus sp. nov. and Vibrio wodanis sp. nov. isolated from Atlantic salmon (Salmo salar) with ‘winter ulcer’. Int. J. Syst. Evol. Microbiol., 50: 427–450.

MacPherson H L, Bergh Ø, Birkbeck T H. 2012. An aerolysinlike enterotoxin from Vibrio splendidus may be involved in intestinal tract damage and mortalities in turbot, Scophthalmus maximus (L.), and cod, Gadus morhua L., larvae. J. Fish Dis., 35(2): 153–167.

Montes M, Farto R, Pérez M J, Nieto T P, Larsen J L, Christensen H. 2003. Characterization of Vibrio strains isolated from turbot (Scophthalmus maximus) culture by phenotypic analysis, ribotyping and 16S rRNA gene sequence comparison. J. Appl. Microbiol., 95(4): 693–703.

Navarrete P, Espejo R T, Romero J. 2009. Molecular analysis of microbiota along the digestive tract of juvenile Atlantic salmon (Salmo salar L.). Microb. Ecol., 57(3): 550–561.

Nayak S K. 2010. Probiotics and immunity: a fish perspective. Fish Shellfish Immunol., 29(1): 2–14.

Nichols D, Lewis K, Orjala J, Mo S, Ortenberg R, O’Connor P, Zhao C, Vouros P, Kaeberlein T, Epstein S S. 2008. Short peptide induces an “uncultivable” microorganism to grow in vitro. Appl. Environ. Microbiol., 74(15): 4 889–4 897.

Pérez T, Balcázar J L, Ruiz-Zarzuela I, Halaihel N, Vendrell D, de Blas I, Múzquiz J L. 2010. Host-microbiota interactions within the fish intestinal ecosystem. Mucosal Immunol., 3: 355–360.

Pérez-Sánchez T, Balcázar J L, García Y, Halaihel N, Vendrell D, de Blas I, Merrifield D L, Ruiz-Zarzuela I. 2011. Identification and characterization of lactic acid bacteria isolated from rainbow trout, Oncorhynchus mykiss (Walbaum), with inhibitory activity against Lactococcus garvieae. J. Fish Dis., 34(7): 499–507.

Planas M, Pérez-Lorenzo M, Hjelm M, Gram L, Fiksdal I U, Bergh Ø, Pintado J. 2006. Probiotic effect in vivo of Roseobacter strain 27-4 against Vibrio (Listonella) anguillarum infections in turbot (Scophthalmus maximus L.) larvae. Aquaculture, 255: 323–333.

Ravi A V, Musthafa K S, Jegathammbal G, Kathiresan K, Pandian S K. 2007. Screening and evaluation of probiotics as a biocontrol agent against pathogenic Vibrios in marine aquaculture. Lett. Appl. Microbiol., 45(2): 219–223.

Ringø E, Olsen R E, Mayhew T M, Myklebust R. 2003. Electron microscopy of the intestinal microflora of fish. Aquaculture, 227: 395–415.

Rombout J H, Abelli L, Picchietti S, Scapigliati G, Kiron V. 2011. Teleost intestinal immunology. Fish Shellfish Immunol., 31(5): 616–626.

Shigematsu T, Hayashi M, Kikuchi I, Ueno S, Masaki H, Fujii T. 2009. A culture-dependent bacterial community structure analysis based on liquid cultivation and its application to a marine environment. FEMS. Microbiol. Lett., 293(2): 240–247.

Sipkema D, Schippers K, Maalcke W J, Yang Y, Salim S, Blanch H W. 2011. Multiple approaches to enhance the cultivability of bacteria associated with the marine sponge Haliclona (Gellius) sp. Appl. Environ. Microbiol., 77(6): 2 130–2 140.

Smriga S, Sandin S A, Azam F. 2010. Abundance, diversity, and activity of microbial assemblages associated with coral reef fish guts and feces. FEMS. Microbiol. Ecol., 73: 31–42.

Song J, Oh H M, Cho J C. 2009. Improved culturability of SAR11 strains in dilution-to-extinction culturing from the East Sea, West Pacific Ocean. FEMS. Microbiol. Lett., 295(2): 141–147.

Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol., 24: 1 596–1 599.

Tapia-Paniagua S T, Chabrillón M, Díaz-Rosales P, de la Banda I G, Lobo C, Balebona M C, Moriñigo M A. 2010.

Intestinal microbiota diversity of the flat fish Solea senegalensis (Kaup, 1858) following probiotic administration. Microb. Ecol., 60: 310–319.

Thompson F L, Hoste B, Thompson C C, Goris J, Gomez-Gil B, Huys L, de Vos P, Swings J. 2002. Enterovibrio norvegicus gen. nov., sp. nov., isolated from the gut of turbot (Scophthalmus maximus) larvae: a new member of the family Vibrionaceae. Int. J. Syst. Evol. Microbiol., 52: 2 015–2 022.

Thompson F L, Iida T, Swings J. 2004. Biodiversity of vibrios. Microbiol. Mol. Biol. Rev., 68(3): 403–431.

Urbanczyk H, Ast J C, Higgins M J, Carson J, Dunlap P V. 2007. Reclassification of Vibrio fischeri, Vibrio loge i, Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio salmonicida comb. nov. and Aliivibrio wodanis comb. nov. Int. J. Syst. Evol. Microbiol., 57: 2 823–2 829.

Vartoukian S R, Palmer R M, Wade W G. 2010. Strategies for culture of ‘unculturable’ bacteria. FEMS Microbiol. Lett., 309(1): 1–7.

Villamil L, Tafalla C, Fiqueras A, Novoa B. 2002. Evaluation of immunomodulatory effects of lactic acid bacteria in turbot (Scophthalmus maximus). Clin. Diagn. Lab. Immunol., 9(6): 1 318–1 323.

Vine N G, Leukes W D, Kaiser H. 2004. In vitro growth characteristics of five candidate aquaculture probiotics and two fish pathogens grown in fish intestinal mucus. FEMS. Microbiol. Lett., 231(1): 145–152.

Ward N L, Steven B, Penn K, Methé B A, Detrich W H 3rd. 2009. Characterization of the intestinal microbiota of two Antarctic notothenioid fish species. Extremophiles. 13(4): 679–685.

Wu S, Tian J, Wang G, Li W, Zou H. 2012b. Characterization of bacterial community in the stomach of yellow catfish (Pelteobagrus fulvidraco). World J. Microbiol. Biotechnol., 28: 2 165–2 174.

Wu S, Wang G, Angert E R, Wang W, Li W, Zou H. 2012a. Composition, diversity, and origin of the bacterial community in grass carp intestine. PLoS One, 7: e30440.

Zhu L, Wu Q, Dai J, Zhang S, Wei F. 2011. Evidence of cellulose metabolism by the giant panda gut microbiome. PNAS, 108: 17 714–17 719.

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