A cadmium metallothionein gene of ridgetail white prawn Exopalaemon carinicauda (Holthuis, 1950) and its expression

Jiquan Zhang; Jing Wang; Jianhai Xiang

doi:10.1007/s00343-013-3089-8

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A cadmium metallothionein gene of ridgetail white prawn Exopalaemon carinicauda (Holthuis, 1950) and its expression

OriginalPaper | Updated：2023-05-16

- A cadmium metallothionein gene of ridgetail white prawn Exopalaemon carinicauda (Holthuis, 1950) and its expression
- A cadmium metallothionein gene of ridgetail white prawn Exopalaemon carinicauda (Holthuis, 1950) and its expression
- 海洋湖沼学报（英文） 2013年31卷第6期页码：1204-1209
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 311720449), the National High Technology Research and Development Program of China (No. 2012AA10A401), the Scientific Research Foundation for the Excellent Middle-Aged and Youth Scientists of Shandong Province of China (No. BS2010SW039)
- DOI：10.1007/s00343-013-3089-8
  中图分类号：
- 网络首发：2013-11-06，
  
  纸质出版：2013
- Accepted：
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A cadmium metallothionein gene of ridgetail white prawn Exopalaemon carinicauda (Holthuis, 1950) and its expression[J]. 海洋湖沼学报（英文）, 2013,31(6):1204-1209.

Zhang, J., Wang, J. & Xiang, J. A cadmium metallothionein gene of ridgetail white prawn Exopalaemon carinicauda (Holthuis, 1950) and its expression., Chin. J. Ocean. Limnol. 31, 1204–1209 (2013). https://doi.org/10.1007/s00343-013-3089-8
A cadmium metallothionein gene of ridgetail white prawn Exopalaemon carinicauda (Holthuis, 1950) and its expression[J]. 海洋湖沼学报（英文）, 2013,31(6):1204-1209. DOI： 10.1007/s00343-013-3089-8.

Zhang, J., Wang, J. & Xiang, J. A cadmium metallothionein gene of ridgetail white prawn Exopalaemon carinicauda (Holthuis, 1950) and its expression., Chin. J. Ocean. Limnol. 31, 1204–1209 (2013). https://doi.org/10.1007/s00343-013-3089-8 DOI：

摘要

Abstract

Metallothioneins (MTs) are a group of low molecular weight cysteine-rich proteins capable of binding heavy metal ions. A cadmium metallothionein (EcMT — Cd) cDNA with a 189 bp open reading frame (ORF) that encoded a 62 amino acid protein was obtained from Exopalaemon carinicauda. Seventeen cysteines were in the deduced amino acid sequence

and the cysteine (Cys)-rich characteristic was revealed in different metallothioneins in other species. In addition

the deduced amino acid sequence did not contain any aromatic amino acid residues

such as tyrosine (Tyr)

tryptophan (Trp)

and phenylalanine (Phe). EcMT—Cd mRNA was expressed in all tested tissues (the ovary

muscle

stomach

and hepatopancreas)

and its expression profiles in the hepatopancreas were very different when shrimps were exposed to seawater containing either 50 μmol/L CuSO4 or 2.5 μmol/L CdCl 2. The expression of EcMT-Cd was significantly up-regulated in shrimp exposed to CuSO4 for 12 h and down-regulated in shrimps exposed to CdCl2 for 12 h. After 24 h exposure to both metals

its expression was down-regulated. By contrast

at 48 h the EcMT-Cd was up-regulated in test shrimps exposed to CdCl2. The transcript of EcMT-Cd was very low or even absent before the zoea stage

and the expression of EcMT-Cd was detected from mysis larvae-I

then its expression began to rise. In conclusion

a cadmium MT exists in E. carinicauda that is expressed in different tissues and during different developmental stages

and responds to the challenge with heavy metal ions

which provides a clue to understanding the function of cadmium MT.

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references

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