Purification and characterization of iron-cofactored superoxide dismutase from Enteromorpha linza

Mingsheng Lü; Ruanhong Cai; Shujun Wang; Zhaopu Liu; Yuliang Jiao; Yaowei Fang; Xiaoxin Zhang

doi:10.1007/s00343-013-3049-3

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Purification and characterization of iron-cofactored superoxide dismutase from Enteromorpha linza

OriginalPaper | Updated：2023-05-16

- Purification and characterization of iron-cofactored superoxide dismutase from Enteromorpha linza
- Purification and characterization of iron-cofactored superoxide dismutase from Enteromorpha linza
- 海洋湖沼学报（英文） 2013年31卷第6期页码：1190-1195
- Affiliations：
  
  1. Marine School Huaihai Institute of Technology
  2. College of Resources and Environmental Science Nanjing Agricultural University
- Author bio：
- Funds：
  
  Supported by the National Key Technology R&D Program of China (No. 2012BAC07B03)
- DOI：10.1007/s00343-013-3049-3
  中图分类号：
- 网络首发：2013-08-31，
  
  纸质出版：2013
- Accepted：
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Purification and characterization of iron-cofactored superoxide dismutase from Enteromorpha linza[J]. 海洋湖沼学报（英文）, 2013,31(6):1190-1195.

Lü, M., Cai, R., Wang, S. et al. Purification and characterization of iron-cofactored superoxide dismutase from Enteromorpha linza, ., Chin. J. Ocean. Limnol. 31, 1190–1195 (2013). https://doi.org/10.1007/s00343-013-3049-3
Purification and characterization of iron-cofactored superoxide dismutase from Enteromorpha linza[J]. 海洋湖沼学报（英文）, 2013,31(6):1190-1195. DOI： 10.1007/s00343-013-3049-3.

Lü, M., Cai, R., Wang, S. et al. Purification and characterization of iron-cofactored superoxide dismutase from Enteromorpha linza, ., Chin. J. Ocean. Limnol. 31, 1190–1195 (2013). https://doi.org/10.1007/s00343-013-3049-3 DOI：

摘要

Abstract

A superoxide dismutase was purified from Enteromorpha linza using a simple and safe procedure

which comprised phosphate buffer extraction

ammonium sulphate precipitation

ion exchange chromatography on Q-sepharose column

and gel filtration chromatography on Superdex 200 10/300GL. The E. linza superoxide dismutase (ElSOD) was purified 103.6-fold

and a yield of 19.1% and a specific activity of 1 750 U/mg protein were obtained. The SDS-PAGE exhibited ElSOD a single band near 23 kDa and the gel filtration study showed ElSOD’s molecular weight is near 46 kDa in nondenatured condition

indicating it’s a homodimeric protein. El SOD is an iron-cofactored superoxide dismutase (Fe-SOD) because it was inhibited by hydrogen peroxide

insensitive to potassium cyanide. The optimal temperature for its maximal enzyme activity was 35°C

and it still had 29.8% relative activity at 0°C

then ElSOD can be classified as a cold-adapted enzyme. ElSOD was stable when temperature was below 40°C or the pH was within the range of 5–10. The first 11 N-terminal amino acids of ElSOD were ALELKAPPYEL

comparison of its N-terminal sequence with other Fe-SOD N-terminal sequences at the same position suggests it is possibly a chloroplastic Fe-SOD.

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references

Asensio A C, Gil-Monreal M, Pires L, Gogorcena Y, Aparicio-Tejo P M, Moran J F. 2012. Two Fe-superoxide dismutase families respond differently to stress and senescence in legumes. J. Plant Physiol., 169(13): 1 253–1 260.

Boyadzhieva I P, Atanasova M, Emanuilova E. 2010. A novel, thermostable manganese-containing superoxide dismutase from Bacillus licheniformis. Biotechnol. Lett., 32(12): 1 893–1 896.

Bradford M M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. biochem., 72: 248–254.

Bridges S M, Salin M L. 1981. Distribution of iron-containing superoxide dismutase in vascular plants. Plant Physiol., 68(2): 275–278.

Ghosh N, Ghosh R, Mandal V, Mandal S C. 2011. Recent advances in herbal medicine for treatment of liver diseases. Pharm. Biol., 49(9): 970–988.

He N, Li Q, Sun D, Ling X. 2008. Isolation, purification and characterization of superoxide dismutase from garlic. Biochem. Eng. J., 38(1): 33–38.

Il Kwon S, An C S. 2006. Differential expression of two SOD (Superoxide dismutase) genes from small radish (Rhaphanus sativus L. var. sativus). J. Plant. Biol., 49(6): 477–483.

Kitayama K, Kitayama M, Osafune T, Togasaki R K. 1999. Subcellular localization of iron and manganese superoxide dismutase in Chlamydomonas reinhardtii (Chlorophyceae). J. Phycol., 35(1): 136–142.

Kliebenstein D J, Monde R, Last R L. 1998. Superoxide dismutase in Arabidopsis: an eclectic enzyme family with disparate regulation and protein localization. Plant Physiol., 118(2): 637–650.

Laemmli U K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680–685.

Leliaert F, Zhang X, Ye N, Malta E-j, Engelen A H, Mineur F, Verbruggen H, De Clerck O. 2009. Research note: identity of the Qingdao algal bloom. Phycol. Res., 57(2): 147–151.

Liu D, Keesing J K, Dong Z, Zhen Y, Di B, Shi Y, Fearns P, Shi P. 2010. Recurrence of the world’s largest green-tide in 2009 in Yellow Sea, China: Porphyra yezoensis aquaculture rafts confirmed as nursery for macroalgal blooms. Mar. Pollut. Bull., 60(9): 1 423–1 432.

Liu J, Wang J, Yin M, Zhu H, Lu J, Cui Z. 2011. Purification and characterization of superoxide dismutase from garlic. Food Bioprod. Process., 89(4): 294–299.

Margesin R, Schinner F. 1994. Properties of cold-adapted microoganisms and their potential in biotechnology. J. Biotechnol., 33(1): 1–14.

McCord J M, Fridovich I. 1988. Superoxide-dismutase-the 1 st 20 years (1968-1988). Free Radical Bio. Med., 5(6): 363–369.

Moran J F, James E K, Rubio M C, Sarath G, Klucas R V, Becana M. 2003. Functional characterization and expression of a cytosolic iron-superoxide dismutase from cowpea root nodules. Plant Physiol., 133: 773–782.

Priya B, Premanandh J, Dhanalakshmi R T, Seethalakshmi T, Uma L, Prabaharan D, Subramanian G. 2007. Comparative analysis of cyanobacterial superoxide dismutases to discriminate canonical forms. BMC Genomics, 8: 435.

Prochnik S E, Umen J, Nedelcu A M, Hallmann A, Miller S M, Nishii I, Ferris P, Kuo A, Mitros T, Fritz-Laylin L K, Hellsten U, Chapman J, Simakov O, Rensing S A, Terry A, Pangilinan J, Kapitonov V, Jurka J, Salamov A, Shapiro H, Schmutz J, Grimwood J, Lindquist E, Lucas S, Grigoriev I V, Schmitt R, Kirk D, Rokhsar D S. 2010. Genomic analysis of organismal complexity in the multicellular green alga Volvox carteri. Science, 329(5988): 223–226.

Qin Y, Dai W, Wang Y, Gong X G, Lu M. 2013. Fe-SOD cooperates with Nutlin3 to selectively inhibit cancer cells in vitro and in vivo. Biochem. Bioph. Res. Co., 431(2): 169–175.

Schott E J, Robledo J F, Wright A C, Silva A M, Vasta G R. 2003. Gene organization and homology modeling of two iron superoxide dismutases of the early branching protest Perkinsus marinus. Gene, 309(1):1–9.

Thompson J D, Higgins D G, Gibson T J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic. Acids Res., 22: 4 673–4 680.

Wang S, Shao B, Liu S, Ye X, Rao P. 2012. Purification and characterization of Cu, Zn-superoxide dismutase from black soybean. Food Res. Int., 47: 374–379.

Wang Y, Mo X, Zhang L, Wang Q. 2011. Four superoxide dismutase (isozymes) genes of Bacillus cereus. Ann. Microbiol., 61(2): 355–360.

Wu T-M, Hsu Y-T, Sung M-S, Hsu Y-T, Lee T-M. 2009. Expression of genes involved in redox homeostasis and antioxidant defense in a marine macroalga Ulva fasciata by excess copper. A quat. Toxicol., 94(4): 275–285.

Zheng Z, Jiang Y H, Miao J L, Wang Q F, Zhang B T, Li G Y. 2006. Purification and characterization of a cold-active iron superoxide dismutase from a psychrophilic bacterium, Marinomonas sp. NJ522. Biotechnol. Lett., 28(2): 85–88.

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