First record of Biecheleria cincta (Dinophyceae) from Chinese coasts, with morphological and molecular characterization of the strains

Zhaohe Luo; Weidong Yang; Bin Xu; Haifeng Gu

doi:10.1007/s00343-013-2315-8

Your Location：

Home >

Browse articles >

First record of Biecheleria cincta (Dinophyceae) from Chinese coasts, with morphological and molecular characterization of the strains

OriginalPaper | Updated：2023-05-16

- First record of Biecheleria cincta (Dinophyceae) from Chinese coasts, with morphological and molecular characterization of the strains
- First record of Biecheleria cincta (Dinophyceae) from Chinese coasts, with morphological and molecular characterization of the strains
- 海洋湖沼学报（英文） 2013年31卷第4期页码：835-845
- Affiliations：
  
  1. College of Life Science and Technology Jinan University
  2. Third Institute of Oceanography SOA
  3. Institute of Urban Environment Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Scientific-Basic Special Fund (No. 2009FY210400)
- DOI：10.1007/s00343-013-2315-8
  中图分类号：
- 网络首发：2013-07-10，
  
  纸质出版：2013
- Accepted：
Scan QR Code
First record of Biecheleria cincta (Dinophyceae) from Chinese coasts, with morphological and molecular characterization of the strains[J]. 海洋湖沼学报（英文）, 2013,31(4):835-845.

Luo, Z., Yang, W., Xu, B. et al. First record of Biecheleria cincta (Dinophyceae) from Chinese coasts, with morphological and molecular characterization of the strains., Chin. J. Ocean. Limnol. 31, 835–845 (2013). https://doi.org/10.1007/s00343-013-2315-8
First record of Biecheleria cincta (Dinophyceae) from Chinese coasts, with morphological and molecular characterization of the strains[J]. 海洋湖沼学报（英文）, 2013,31(4):835-845. DOI： 10.1007/s00343-013-2315-8.

Luo, Z., Yang, W., Xu, B. et al. First record of Biecheleria cincta (Dinophyceae) from Chinese coasts, with morphological and molecular characterization of the strains., Chin. J. Ocean. Limnol. 31, 835–845 (2013). https://doi.org/10.1007/s00343-013-2315-8 DOI：

摘要

Abstract

The presence of Biecheleria cincta (= Woloszynskia cincta) in the Chinese coasts is reported for the first time. In scanning electron microscope

three to five series of vesicles and an elongated apical vesicle (EAV) were visible in the epicone

and both the hypocone and the cingulum had three series of vesicles each. Thin sections revealed that B. cincta possesses stalked pyrenoids and an unusual eyespot consisting of a stack of cisternae with brick-like materials (type E)

thus supporting its transfer from Woloszynskia to Biecheleria. Spiny cysts formed spontaneously in culture

with an encystment rate of around 20%. Both large subunit ribosomal DNA (LSU rDNA) and internal transcribed spacer region (ITS) sequences in 12 strains from the Chinese coasts were determined. Phylogenetic analyses based on LSU rDNA and ITS sequences using Bayesian inference and maximum likelihood revealed two distinct ribotypes (referred to as ribotype A and B) in B. cincta. ITS region pairwise distances within B. cincta ranged from 0.024 to 0.072

suggesting the existence of a complex of cryptic species.

关键词

Keywords

references

Adachi M, Sake Y, Ishida Y. 1996. Analysis of A l exandrium (Dinophyceae) species using sequences of the 5.8S ribosomal DNA and internal transcribed spacer regions. Journal of Phycology, 32(3): 424–432.

Balzano S, Gourvil P, Siano R, Chanoine M, Marie D, Lessard S, Sarno D, Vaulot D. 2012. Diversity of cultured photosynthetic flagellates in the North East Pacific and Arctic Oceans in summer. Biogeosciences Discuss ions, 9: 6 219–6 259.

Biecheler B. 1952. Recherches sur les Péridiniens. Bulletin Biologique France Belgique, 36(Suppl.): 1–149.

Brosnahan M L, Kulis D M, Solow A R, Erdner D L, Percy L, Lewis J, Anderson D M. 2010. Outbreeding lethality between toxic Group I and nontoxic Group III Alexandrium tamarense spp. isolates: predominance of heterotypic encystment and implications for mating interactions and biogeography. Deep Sea Research Part II: Topical Studies in Oceanography, 57(3): 175–189.

Calado A J, Craveiro S C, Daugbjerg N, Moestrup Ø. 2006. Ultrastructure and LSU rDNA-based phylogeny of Esoptrodinium gemma (Dinophyceae), with notes on feeding behavior and the description of the flagellar base area of a planozygote. Journal of Phycology, 42: 434–452.

Calado A J, Moestrup Ø. 2005. On the freshwater dinoflagellates presently included in the genus Amphidinium, with a description of Prosoaulax gen. nov. Phycologia, 44(1): 112–119.

Calado A J. 2011. On the identity of the freshwater dinoflagellate Glenodinium edax, with a discussion on the genera Tyrannodinium and Katodinium, and the description of Opisthoaulax gen. nov. Phycologia, 50(6): 641–649.

Casteleyn G, Adams N G, Vanormelingen P, Debeer A E, Sabbe K, Vyverman W. 2009. Natural hybrids in the marine diatom Pseudo-nitzschia pungens (Bacillariophyceae): genetic and morphological evidence. Protist, 160(2): 343–354.

Casteleyn G, Chepurnov V A, Leliaert F, Mann D G, Bates S S, Lundholm N, Rhodes L, Sabbe K, Vyverman W. 2008. Pseudo-nitzschia pungens (Bacillariophyceae): a cosmopolitan diatom species? Harmful Algae, 7(2): 241–257.

D’Alelio D, Amato A, Kooistra W H C F, Procaccini G, Casotti R, Montresor M. 2009. Internal transcribed spacer polymorphism in Pseudo-nitzschia multistriata(Bacillariophyceae) in the Gulf of Naples: recent divergence or intraspecific hybridization? Protist, 160(1): 9–20.

Freudenthal H D. 1962. Symbiodinium gen. nov. and Symbiodinium microadriaticum sp. nov., a zooxanthella: taxonomy, life cycle, and morphology. Journal of Protozoology, 9(1): 45–52.

Granados-Cifuentes C, Rodriguez-Lanetty M. 2011. The use of high-resolution melting analysis for genotyping Symbiodinium strains: a sensitive and fast approach. Molecular Ecology Resources, 11(2): 394–399.

Guillard R R L, Ryther J H. 1962. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and D etonula confervacea (Cleve) Gran. Canadian Journal of Microbiology, 8(2): 229–239.

Hall T A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41: 95–98.

Hansen G, Daugbjerg N, Henriksen P. 2007. Baldinia anauniensis gen. et sp. nov.: a ‘new’ dinoflagellate from Lake Tovel, N. Italy. Phycologia, 46(1): 86–108.

Hansen G, Daugbjerg N. 2009. Symbiodinium natans sp. nov.: a “free-living” dinoflagellate from Tenerife (Northeast-Atlantic Ocean). Journal of Phycology, 45(1): 251–263.

Hillis D M, Dixon M T. 1991. Ribosomal DNA: molecular evolution and phylogenetic inference. Quarterly Review of Biology, 66(4): 411–453.

Horiguchi T, Pienaar R. 1994. Gymnodinium natalense sp. nov. (Dinophyceae), a new tide pool dinoflagellate from South Africa. Japanese Journal of Phycology, 42: 21–28.

Kang N S, Jeong H J, Yoo Y D, Yoon E Y, Lee K H, Lee K, Kim G. 2011. Mixotrophy in the newly described phototrophic dinoflagellate Woloszynskia cincta from western Korean waters: feeding mechanism, prey species and effect of prey concentration. Journal of Eukaryotic Microbiology, 58(2): 152–170.

Katoh K, Kuma K, Toh H, Miyata T. 2005. MAFFT version 5: improvement in accuracy of multiple sequence alignment. Nucleic Acids Research, 33(2): 511–518.

Kremp A, Elbrächter M, Schweikert M, Wolny J L, Gottschling M. 2005. Woloszynskia halophila (Biecheler) comb. nov.: a bloom-forming cold-water dinoflagellate co-occurring with S crippsiella hangoei (Dinophyceae) in the Baltic Sea. Journal of Phycology, 41(3): 629–642.

Lindberg K, Moestrup Ø, Daugbjerg N. 2005. Studies on woloszynskioid dinoflagellates I: Woloszynskia coronata re-examined using light and electron microscopy and partial LSU rDNA sequences, with description of Tovellia gen. nov. and Jadwigia gen. nov. (Tovelliaceae fam. nov.). Phycologia, 44(4): 416–440.

Litaker R W, Vandersea M W, Kibler S R, Reece K S, Stokes N A, Lutzoni F M, Yonish B A, West M A, Black M N D, Tester P A. 2007. Recognizing dinoflagellate species using ITS rDNA sequences. Journal of Phycology, 43(2): 344–355.

Loeblich A R, Sherley J L. 1979. Observations on the theca of the motile phase of free-living and symbiotic isolates of Zooxanthella microadriatica (Freudenthal) comb. nov. Journal of the Marine Biological Association of the United Kingdom, 59(1): 195–205.

Moestrup Ø, Daugbjerg N. 2007. On dinoflagellate phylogeny and classification. In: Brodie J, Lewis J eds. Unravelling the Algae: the Past, Present, and Future of Algal Systematics. CRC Press. Boca Raton, FL. p.215–230.

Moestrup Ø, Hansen G, Daugbjerg N. 2008. Studies on woloszynskioid dinoflagellates III: on the ultrastructure and phylogeny of Borghiella dodgei gen. et sp. nov., a cold-water species from Lake Tovel, N. Italy, and on B. tenuissima comb. nov. (syn. Woloszynskia tenuissima). Phycologia, 47(1): 54–78.

Moestrup Ø, Lindberg K, Daugbjerg N. 2009a. Studies on woloszynskioid dinoflagellates IV: the genus Biecheleria gen. nov. Phycological Research, 57(3): 203–220.

Moestrup Ø, Lindberg K, Daugbjerg N. 2009b. Studies on woloszynskioid dinoflagellates V. Ultrastructure of Biecheleriopsis gen. nov., with description of Biecheleriopsis adriatica sp. nov. Phycological Research, 57(3): 221–237.

Montresor M, Lovejoy C, Orsini L, Procaccini G, Roy S. 2003. Bipolar distribution of the cyst-forming dinoflagellate Polarella glacialis. Polar Biology, 26(3): 186–194.

Montresor M, Procaccini G, Stoecker D K. 1999. Polarella glacialis, gen. nov., sp. nov. (Dinophyceae): Suessiaceae are still alive! Journal of Phycology, 35(1): 186–197.

Nylander J A A, Wilgenbusch J C, Warren D L, Swofford D L. 2008. AWTY (are we there yet?): a system for graphical exploration of MCMC convergence in Bayesian phylogenetics. Bioinformatics, 24(4): 581–583.

Posada D. 2008. JModelTest: phylogenetic model averaging. Molecular Biology and Evolution, 25(7): 1 253–1 256.

Ronquist F, Huelsenbeck J P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19(12): 1 572–1 574.

Schiller J. 1933. Dinoflagellatae (Peridineae) in monographischer Behandlung, 1.Theil. In: Rabenhorst L ed. Kryptogamen-Flora von Deutschland, Österreich und der Schweiz. Akademische Verlagsgesellschaft. Leipzig, Germany. 617p.

Scholin C A, Herzog M, Sogin M, Anderson D M. 1994. Identification of group- and strain-specific genetic markers for globally distributed Alexandrium (dinophyceae). II. sequence analysis of a fragment of the LSU rRNA gene. Journal of Phycology, 30(6): 999–1 011.

Siano R, Kooistra W H C F, Montresor M, Zingone A. 2009. Unarmoured and thin-walled dinoflagellates from the Gulf of Naples, with the description of Woloszynskia cincta sp. nov. (Dinophyceae, Suessiales). Phycologia, 48(1): 44–65.

Spurr A R. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research, 26: 31–43.

Stamatakis A, Hoover P, Rougemont J. 2008. A rapid bootstrap algorithm for the RAxML Web servers. Systematic Biology, 57(5): 758–771.

Swofford D. 2002. Sinauer Associates; Sunderland, MA: 2002. PAUP*. Phylogenetic analysis using parsimony (* and other methods), version 4, b10.

Thompson R. 1951. A new genus and new records of freshwater Pyrrophyta in the Desmokontae and Dinophyceae. Lloydia, 13(4): 277–299.

Trench R K, Blank R J. 1987. Symbiodinium microadriaticum freudenthal, S. goreauii sp. nov., S. kawagutii sp. nov. and S. pilosum sp. nov.: gymnodinioid dinoflagellate symbionts of marine invertebrates. Journal of Phycology, 23(3): 469–481.

Zinssmeister C, Soehner S, Facher E, Kirsch M, Meier K J S, Gottschling M. 2011. Catch me if you can: the taxonomic identity of Scrippsiella trochoidea (F. Stein) AR Loebl. (Thoracosphaeraceae, Dinophyceae). Systematics and Biodiversity, 9(2): 145–157.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Molecular phylogeny and ultrastructure of a new population of Urostyla grandis (Ciliophora, Hypotrichida) from Harbin, China

Morphological and functional studies on the epidermal cells of amphioxus (Branchiostoma belcheri tsingtauense) at different developmental stages

On the ultrastructure and classification of the hemocytes of penaeid shrimp,Penaeus vannamei (Crustacea, Decapoda)

The ultrastructure of separated and cultured cell ofPorphyra yezoensis

Further observation of the spermatozoa of lefteye flounderParalichthys olivaceus by electronic microscopy