Effect of cadmium on the defense response of Pacific oyster Crassostrea gigas to Listonella anguillarum challenge

Ming Cong; Jiasen Lü; Huifeng Wu; Jianmin Zhao

doi:10.1007/s00343-013-2280-2

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Effect of cadmium on the defense response of Pacific oyster Crassostrea gigas to Listonella anguillarum challenge

OriginalPaper | Updated：2023-05-16

- Effect of cadmium on the defense response of Pacific oyster Crassostrea gigas to Listonella anguillarum challenge
- Effect of cadmium on the defense response of Pacific oyster Crassostrea gigas to Listonella anguillarum challenge
- 海洋湖沼学报（英文） 2013年31卷第5期页码：1002-1009
- Affiliations：
  
  1. Key Laboratory of Coastal Zone Environment Processes Chinese Academy of Sciences
  2. Shandong Provincial Key Laboratory of Coastal Zone Environment Processes Yantai Institute of Coastal Zone Research Chinese Academy of Sciences
  3. Biology School of Yantai University
- Author bio：
- Funds：
  
  Supported by the 100 Talents Program of Chinese Academy of Sciences, the Development Plan of Science and Technology in Shandong Province (No. 2012GGA06032), and the Key Deployment Program of Chinese Academy of Sciences (No. KZZD-EW-14-03)
- DOI：10.1007/s00343-013-2280-2
  中图分类号：
- 网络首发：2013-09-07，
  
  纸质出版：2013
- Accepted：
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Effect of cadmium on the defense response of Pacific oyster Crassostrea gigas to Listonella anguillarum challenge[J]. 海洋湖沼学报（英文）, 2013,31(5):1002-1009.

Cong, M., Lü, J., Wu, H. et al. Effect of cadmium on the defense response of Pacific oyster Crassostrea gigas to Listonella anguillarum challenge., Chin. J. Ocean. Limnol. 31, 1002–1009 (2013). https://doi.org/10.1007/s00343-013-2280-2
Effect of cadmium on the defense response of Pacific oyster Crassostrea gigas to Listonella anguillarum challenge[J]. 海洋湖沼学报（英文）, 2013,31(5):1002-1009. DOI： 10.1007/s00343-013-2280-2.

Cong, M., Lü, J., Wu, H. et al. Effect of cadmium on the defense response of Pacific oyster Crassostrea gigas to Listonella anguillarum challenge., Chin. J. Ocean. Limnol. 31, 1002–1009 (2013). https://doi.org/10.1007/s00343-013-2280-2 DOI：

摘要

Abstract

Heavy metal pollution can affect the immune capability of organisms. We evaluated the effect of cadmium (Cd) on the defense responses of the Pacific oyster Crassostrea gigas to Listonella anguillarum challenge. The activities of several important defensive enzymes

including superoxide dismutase (SOD)

glutathione peroxidase (GPx)

acid phosphatase (ACP)

Na+

K+ -ATPase in gills and hepatopancreas

and phenoloxidase-like (POL) enzyme in hemolymph were assayed. In addition

the expression levels of several genes

including heat shock protein 90 (HSP90)

metallothionein (MT)

and bactericidal/permeability increasing (BPI) protein were quantified by fluorescent quantitative PCR. The enzyme activities of SOD

ACP

POL

and GPx in hepatopancreas

and the expression of HSP90 were down-regulated

whereas GPx activity in the gill

Na+

K+-ATPase activities in both tissues

and MT expression was increased in Cdexposed oysters post L. anguillarum challenge. However

BPI expression was not significantly altered by co-stress of L. anguillarum infection and cadmium exposure. Our results suggest that cadmium exposure alters the oysters’ immune responses and energy metabolism following vibrio infection.

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references

Aladaileh S, Nair S V, Birch D, Raftos D A. 2007. Sydney rock oyster (Saccostrea glomerata) hemocytes: morphology and function. J. Invertebr. Pathol., 96: 48–63.

Bado-Nilles A, Renault T, Faury N, Le Floch S, Quentel C, Auffret M, Thomas-Guyon H. 2010. In vivo effects of LCO soluble fraction on immune-related functions and gene transcription in the Pacific oyster, Crassostrea gigas (Thunberg). Aquat. Toxicol., 97: 196–203.

Barral J M, Broadley S A, Schaffar G, Hartl F U. 2004. Roles of molecular chaperones in protein misfolding diseases, Semin. Cell Dev. Biol., 15: 17–29.

Bouilly K, Gagnaire B, Bonnard M, Thomas-Guyon H, Renault T, Miramand P, Lapègue S. 2006. Effects of cadmium on aneuploidy and hemocyte parameters in the Pacific oyster, Crassostrea gigas. Aquat. Toxicol., 78: 149–156.

Brown C. 1981. A prodiginine pigment toxic to embryos and larvae of Crassostrea virginica. J. Invertebr. Pathol., 38: 281–293.

Brulle F, Mitta G, Leroux R, Lemière S, Leprêtre A, Vandenbulcke F. 2007. The strong induction of metallothionein gene following cadmium exposure transiently affects the expression of many genes in Eisenia Fetida: a trade-off mechanism?. Comp. Biochem. Physiol. C, 144: 334–341.

Calabrese E J. 2008. Hormesis: why it is important to toxicology and toxicologists. Environ. Toxicol. Chem., 27: 1 451–1 474.

Cherkasov A S, Biswas P K, Ridings D M, Ringwood A H, Sokolova I M. 2006. Effects of acclimation temperature and cadmium exposure on cellular energy budgets in a marine mollusk Crassostrea virginica: linking cellular and mitochondrial responses. J. Exp. Biol., 209: 1 274–1 284.

Chester R. 1990. Marine Geochemistry, 1st edn. Allen & Unwin, London.

Cong M, Song L, Wang L, Zhao J, Qiu L, Li L, Zhang H. 2008. The enhanced immune protection of zhikong scallop Chlamys farreri on the secondary encounter with Listonella anguillarum. Comp. Biochem. Physiol. B, 151: 191–196.

Cossu C, Doyotte A, Jacquin M C, Babut M, Exinger A, Vasseur P. 1997. Glutathione reductase, seleniumdependent glutathione peroxidase, glutathione levels and lipid peroxidation in freshwater bivalves, Unio turmidus as biomarkers of aquatic contamination in field studies. Ecotox. Environ. Saf., 38: 122–131.

Deng H, Sui X L, Wang Z S. 1992. Primary study on vibrios of the Pacific oyster, Crassostrea gigas. Fish. Sci., 11: 1–4. (in Chinese with English abstract)

Elsbach P, Weiss J. 1993. Bactericida/permeablity increasing protein and host defense against gram-negative bacteria and endotoxin. Curr. Opin. Immunol., 5: 103–107.

Fang Y, Yang H, Wang T, Liu B, Zhao H, Chen M. 2009. Metallothionein and superoxide dismutase responses to sublethal cadmium exposure in the clam Mactra veneriformis. Comp. Biochem. Physiol. C, 151: 325–333.

Flick J G J. 2007. Pathogenic vibrios in shellfish. Global Aquaculture Advocate, 10: 46–48.

Fridovich I. 1975. Superoxide dismutases. Annu. Rev. Biochem., 44: 147–159.

Giannapas M, Karnis L, Dailianis S. 2012. Generation of free radicals in haemocytes of mussels after exposure to low molecular weight PAH components: immune activation, oxidative and genotoxic effects. Comp. Bioc hem. Physiol. C, 155: 182–189.

Hauton C, Hawkins L E, Hutchinson S. 2000. The effects of salinity on the interaction between a pathogen (Listonella anguillarum) and components of a host (Ostrea edulis) immune system. Comp. Biochem. Physiol. B, 127: 203–212.

Ivanina A V, Eilers S, Kurochkin I O, Chung J S, Techa S, Piontkivska H, Sokolov E P, Sokolova I M. 2010. Effects of cadmium exposure and intermittent anoxia on nitric oxide metabolism in eastern oysters, Crassostrea virginica. J. Exp. Biol., 213: 433–444.

Jing G, Li Y, Xie L, Zhang R. 2007. Different effects of Pb2+ and Cu2+ on immune and antioxidant enzyme activities in the mantle of Pinctada fucata. Environ. Toxicol. Phar., 24: 122–128.

Jo P G, Choi Y K, Choi C Y. 2008. Cloning and mRNA expression of antioxidant enzymes in the Pacific oyster, Crassostrea gigas in response to cadmium exposure. Comp. Biochem. Physiol. C, 147: 460–469.

Jorgensen P L, Pedersen P A. 2001. Structure-function relationships of Na+, K+, ATP, or Mg2+ binding and energy transduction in Na, K-ATPase. Biochim. Biophys. Acta, 1505: 57–74.

Kannan K, Jain S K. 2000. Oxidative stress and apoptosis. Pathophysiology, 7: 153–163.

Labreuche Y, Lambert C, Soudant P, Boulo V, Huvet A, Nicolas J. 2006. Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32. Dev. Comp. Immunol., 8: 2 715–2 724.

Li R, Zhou Y, Ji J, Wang L. 2011. Oxidative damages by cadmium and the protective effects of low-molecularweight chitosan in the freshwater crab (Sinopotamon yangtsekiense Bott 1967). Aquac. Res., 42: 506–515.

Liu F, Wang D Z, Wang W X. 2012. Cadmium-induced changes in trace element bioaccumulation and proteomics perspective in four marine bivalves. Environ. Toxicol. Chem., 31: 1 292–1 300.

Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods, 25: 402–408.

Luna-Acosta A, Bustamante P, Godefroy J, Fruitier-Arnaudin I, Thomas-Guyon H. 2010. Seasonal variation of pollution biomarkers to assess the impact on the health status of juvenile Pacific oysters Crassostrea gigas exposed in situ. Environ. Sci. Pollut. Res. Int., 17: 999–1 008.

Masters S L, O’Neill L A J. 2011. Disease-associated amyloid and misfolded protein aggregates activate the inflammasome. Trends Mol. Med., 17: 276–282.

Mattson M P. 2008. Hormesis defined. Ageing Res. Rev., 7: 1–7.

McCord J M. 1993. Human disease, free radicals, and the oxidant/antioxidant balance. Clin. Biochem., 26: 351–357.

Sokolova I M, Evans S, Hughes F M. 2004. Cadmium-induced apoptosis in oyster hemocytes involves disturbance of cellular energy balance but no mitochondrial permeability transition. J. Exp. Biol., 207: 3 369–3 380.

Sokolova I M, Frederich M, Bagwe R, Lannig G, Sukhotin A A. 2012. Energy homeostasis as an integrative tool for assessing limits of environmental stress tolerance in aquatic invertebrates. Mar. Environ. Res., 79: 1–15.

Pena-Llopis S, Ferrando M D. 2003. Fish tolerance to organophosphate-induced oxidative stress is dependent on the glutathione metabolism and enhanced by NH2 — acetylcysteine. Aquat. Toxicol., 65: 337–360.

Pauley G B, Van Der Raay B M, Troutt D. 1988. Species profiles: life histories and environmental requirements of coastal fishes and invertebrates (Pacific Northwest): Pacific oyster. Fish and Wildlife Service Biological Report, 82(11.85): 28.

Qin Y, Meng W, Zheng B, Su Y. 2006. Heavy metal pollution in tidal zones of Bohai Bay using the dated sediment cores. J. Environ. Sci., 18: 610–615. (in Chinese with English abstract)

Sies H. 1993. Strategies of antioxidant defense. Eur. J. Biochem., 215: 213–219.

Thompson F L, Thompson C C, Dias G M, Naka H, Dubay C, Crosa J H. 2011. The genus Listonella Macdonell and Colwell 1986 is a later heterotypic synonym of the genus Vibrio Pacini 1854 (approved lists 1980)-a taxonomic opinion. Int. J. Syst. Evol. Micr., 61: 3 023–3 027.

Verma S R, Rani S, Dalela R C. 1980. Effects of phenol and dinitrophenol on acid and alkaline phosphatases in tissues of a fish, Notopterus notopterus. Arch. Environ. Contam. Toxicol., 9: 451–459.

Wang L, Song L, Ni D, Zhang H, Liu W. 2009. Alteration of metallothionein mrna in bay scallop Argopecten irradians under cadmium exposure and bacteria challenge. Comp. Biochem. Physiol. C, 149: 50–57.

Wu H, Wang W X. 2011. Tissue-specific toxicological effects of cadmium in green mussels (Perna viridis): nuclear magnetic resonance-based metabolomics study. Environ. Toxicol. Chem., 30: 806–812.

Zhang L, Liu X, You L, Zhou D, Yu J, Zhao J, Feng J, Wu H. 2011. Toxicological effects induced by cadmium in gills of manila clam Ruditapes philippinarum using NMRbased metabolomics. Clean Soil Air Water, 39: 989–995.

Zhang X. 2001. Investigation of pollution of Pb, Cd, Hg, as in sea water and deposit of Bohai Sea area. Heilongjiang Environ. J., 25: 87–90. (in Chinese with an English abstract)

Zhang Z, Zhang Q. 2012. Molecular cloning, characterization and expression of heat shock protein 70 gene from the oyster Crassostrea hongkongensis responding to thermal stress and exposure of Cu2+ and malachite green. Gene, 497: 172–180.

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