Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredoxin 2 genes in Mytilus galloprovincialis

Qing Wang; Xuanxuan Ning; Dong Pei; Jianmin Zhao; Liping You; Chunyan Wang; Huifeng Wu

doi:10.1007/s00343-013-2234-8

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Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredoxin 2 genes in Mytilus galloprovincialis

OriginalPaper | Updated：2023-05-16

- Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredoxin 2 genes in Mytilus galloprovincialis
- Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredoxin 2 genes in Mytilus galloprovincialis
- 海洋湖沼学报（英文） 2013年31卷第3期页码：493-503
- Affiliations：
  
  1. Key Laboratory of Coastal Zone Environmental Processes Chinese Academy of Science
  2. Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes Yantai Institute of Coastal Zone Research Chinese Academy of Sciences
  3. Yantai Oceanic Environmental Monitoring Central Station of SOA
  4. China Agriculture University,Yantai
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 31172388), the 100 Talents Program of the Chinese Academy of Sciences, and the Key Laboratory for Ecological Environment in Coastal Areas (201011), State Oceanic Administration
- DOI：10.1007/s00343-013-2234-8
  中图分类号：
- 网络首发：2013-02-01，
  
  纸质出版：2013
- Accepted：
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Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredoxin 2 genes in Mytilus galloprovincialis[J]. 海洋湖沼学报（英文）, 2013,31(3):493-503.

Wang, Q., Ning, X., Pei, D. et al. Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredoxin 2 genes in Mytilus galloprovincialis, ., Chin. J. Ocean. Limnol. 31, 493–503 (2013). https://doi.org/10.1007/s00343-013-2234-8
Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredoxin 2 genes in Mytilus galloprovincialis[J]. 海洋湖沼学报（英文）, 2013,31(3):493-503. DOI： 10.1007/s00343-013-2234-8.

Wang, Q., Ning, X., Pei, D. et al. Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredoxin 2 genes in Mytilus galloprovincialis, ., Chin. J. Ocean. Limnol. 31, 493–503 (2013). https://doi.org/10.1007/s00343-013-2234-8 DOI：

摘要

Abstract

Thioredoxin (Trx) proteins are involved in many biological processes especially the regulation of cellular redox homeostasis. In this study

two Trx cDNAs were cloned from the mussel Mytilus galloprovincialis using rapid amplifi cation of cDNA ends-polymerase chain reaction (RACE-PCR). The two cDNAs were named MgTrx1 and MgTrx2

respectively. The open reading frames of MgTrx1 and MgTrx2 were 318 and 507 base pairs (bp) and they encoded proteins of 105 and 168 amino acids with estimated molecular masses of 11.45 and 18.93 kDa

respectively. Sequence analysis revealed that both proteins possessed the conserved active site dithiol motif Cys-Gly-Pro-Cys. In addition

MgTrx2 also possessed a putative mitochondrial targeting signal suggesting that it is located in the mitochondria. Quantitative real-time polymerase chain reaction (qPCR) revealed that both MgTrx1 and MgTrx2 were constitutively expressed in all tissues examined. The MgTrx1 transcript was most abundant in hemocytes and gills

whereas the MgTrx2 transcript was most abundant in gonad

hepatopancreas

gill and hemocytes. Following Vibrio anguillarum challenge

the expression of MgTrx1 was up-regulated and reached its peak

at a value 10-fold the initial value

at 24 h. Subsequently

expression returned back to the original level. In contrast

the expression level of MgTrx2 was down-regulated following bacterial stimulation

with one fi fth of the control level evident at 12 h post challenge. These results suggest that MgTrx1 and MgTrx2 may play important roles in the response of M. galloprovincialis to bacterial challenge.

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references

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