Fascaplysin sensitizes cells to TRAIL-induced apoptosis through upregulating DR5 expression

Feng Wang; Haimin Chen; Xiaojun Yan; Yanling Zheng

doi:10.1007/s00343-013-2215-y

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Fascaplysin sensitizes cells to TRAIL-induced apoptosis through upregulating DR5 expression

OriginalPaper | Updated：2023-05-16

- Fascaplysin sensitizes cells to TRAIL-induced apoptosis through upregulating DR5 expression
- Fascaplysin sensitizes cells to TRAIL-induced apoptosis through upregulating DR5 expression
- 海洋湖沼学报（英文） 2013年31卷第3期页码：560-569
- Affiliations：
  
  1. Department of Clinical Laboratory Lihuili Hospital of Ningbo Medical Center
  2. Key Laboratory of Applied Marine Biotechnology Ministry of Education Ningbo University
- Author bio：
- Funds：
  
  Supported by the National Science Foundation of Zhejiang Province, China (No. Y5100066), the Ningbo Marine Algae Biotechnology Innovative Team (No. 2011B81007), and the K. C. Wong Magna Fund in Ningbo University;Program for Changjiang Scholars
- DOI：10.1007/s00343-013-2215-y
  中图分类号：
- 网络首发：2013-05-08，
  
  纸质出版：2013
- Accepted：
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Fascaplysin sensitizes cells to TRAIL-induced apoptosis through upregulating DR5 expression[J]. 海洋湖沼学报（英文）, 2013,31(3):560-569.

Wang, F., Chen, H., Yan, X. et al. Fascaplysin sensitizes cells to TRAIL-induced apoptosis through upregulating DR5 expression., Chin. J. Ocean. Limnol. 31, 560–569 (2013). https://doi.org/10.1007/s00343-013-2215-y
Fascaplysin sensitizes cells to TRAIL-induced apoptosis through upregulating DR5 expression[J]. 海洋湖沼学报（英文）, 2013,31(3):560-569. DOI： 10.1007/s00343-013-2215-y.

Wang, F., Chen, H., Yan, X. et al. Fascaplysin sensitizes cells to TRAIL-induced apoptosis through upregulating DR5 expression., Chin. J. Ocean. Limnol. 31, 560–569 (2013). https://doi.org/10.1007/s00343-013-2215-y DOI：

摘要

Abstract

This study investigated the molecular mechanism of anti-tumor effect of fascaplysin

a nitrogenous red pigment firstly isolated from a marine sponge. Microarray analysis show that the TNF and TNF receptor superfamily in human umbilical vein endothelial cells (HUVEC) and human hepatocarcinoma cells (BEL-7402) were significantly regulated by fascaplysin. Western Blot results reveal that fascaplysin increased the expression of cleaved caspase-9

active caspase-3

and decreased the level of procaspase-8 and Bid. Flow cytometry and cytotoxicity tests indicate that fascaplysin sensitized cells to tumor necrosis-related apoptosisinducing ligand-(TRAIL) induced apoptosis

which was markedly blocked by TRAIL R2/Fc chimera

a dominant negative form of TRAIL receptor DR5. Therefore

our results demonstrate that fascaplysin promotes apoptosis through the activation of TRAIL signaling pathway by upregulating DR5 expression.

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references

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