Genetic diversity of populations and clones of Rhopilema esculentum in China based on AFLP analysis

Hongjin Qiao; Xiangquan Liu; Xijia Zhang; Haibin Jiang; Jiying Wang; Limin Zhang

doi:10.1007/s00343-013-2158-3

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Genetic diversity of populations and clones of Rhopilema esculentum in China based on AFLP analysis

OriginalPaper | Updated：2023-05-16

- Genetic diversity of populations and clones of Rhopilema esculentum in China based on AFLP analysis
- Genetic diversity of populations and clones of Rhopilema esculentum in China based on AFLP analysis
- 海洋湖沼学报（英文） 2013年31卷第2期页码：391-397
- Affiliations：
  
  Shandong Marine Fisheries Research Institute Shandong Provincial Key Laboratory of Restoration for Marine Ecology
- Author bio：
- Funds：
  
  Supported by the Taishan Scholarship of Aquatic Animal Nutrition and Feed and the National Marine Public Welfare Research Project (Nos. 201205025, 201305001)
- DOI：10.1007/s00343-013-2158-3
  中图分类号：
- 网络首发：2013-03-07，
  
  纸质出版：2013
- Accepted：
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Genetic diversity of populations and clones of Rhopilema esculentum in China based on AFLP analysis[J]. 海洋湖沼学报（英文）, 2013,31(2):391-397.

Qiao, H., Liu, X., Zhang, X. et al. Genetic diversity of populations and clones of Rhopilema esculentum in China based on AFLP analysis., Chin. J. Ocean. Limnol. 31, 391–397 (2013). https://doi.org/10.1007/s00343-013-2158-3
Genetic diversity of populations and clones of Rhopilema esculentum in China based on AFLP analysis[J]. 海洋湖沼学报（英文）, 2013,31(2):391-397. DOI： 10.1007/s00343-013-2158-3.

Qiao, H., Liu, X., Zhang, X. et al. Genetic diversity of populations and clones of Rhopilema esculentum in China based on AFLP analysis., Chin. J. Ocean. Limnol. 31, 391–397 (2013). https://doi.org/10.1007/s00343-013-2158-3 DOI：

摘要

Abstract

Amplified fragment length polymorphisms (AFLP) markers were developed to assess the genetic variation of populations and clones of Rhopilema esculentum Kishinouye (Scyphozoa

Rhizostomatidae). One hundred and seventy-nine loci from 56 individuals of two hatchery populations and two wild populations were genotyped with five primer combinations. The polymorphic ratio

Shannon’s diversity index and average heterozygosity were 70.3%

0.346 and 0.228 for the white hatchery population

74.3%

0.313

and 0.201 for the red hatchery population

79.3%

0.349

and 0.224 for the Jiangsu wild population

and 74.9%

0.328 and 0.210 for the Penglai wild population

respectively. Thus

all populations had a relatively high level of genetic diversity. A specific band was identified that could separate the white from the red hatchery population. There was 84.85% genetic differentiation within populations. Individual cluster analysis using unweighted pair-group method with arithmetic mean (UPGMA) suggested that hatchery populations and wild populations could be divided. For the hatchery populations

the white and red populations clustered separately; however

for the wild populations

Penglai and Jiangsu populations clustered together. The genetic diversity at the clone level was also determined. Our data suggest that there are relatively high genetic diversities within populations but low genetic differentiation between populations

which may be related to the long-term use of germplasm resources from Jiangsu Province for artificial seeding and releasing. These findings will benefit the artificial seeding and conservation of the germplasm resources.

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references

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