Application of restriction site amplified polymorphism (RSAP) to genetic diversity in Saccharina japonica

Cui Zhao; Cui Liu; Wei Li; Shan Chi; Rongfang Feng; Tao Liu

doi:10.1007/s00343-013-2122-2

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Application of restriction site amplified polymorphism (RSAP) to genetic diversity in Saccharina japonica

OriginalPaper | Updated：2023-05-16

- Application of restriction site amplified polymorphism (RSAP) to genetic diversity in Saccharina japonica
- Application of restriction site amplified polymorphism (RSAP) to genetic diversity in Saccharina japonica
- 海洋湖沼学报（英文） 2013年31卷第4期页码：830-834
- Affiliations：
  
  Laboratory of Genetics and Breeding of Marine Organism College of Marine Life Sciences Ocean University of China
- Author bio：
- Funds：
  
  Supported by the National Special Research Fund for Non-Profit Sector (No. 200805075), the Shandong Foundation for Development of Science and Technology, China (No. 2007GG10005018), the Genetically Modified Organism Technology Major Project (No. 2009ZX08009-100B), the National High Technology Research and Development Program of China (863 Program) (Nos. 2010AA10A401, 2012AA10A406), and the National Agricultural Transformation (No. 2010GB23600666)
- DOI：10.1007/s00343-013-2122-2
  中图分类号：
- 网络首发：2013-07-10，
  
  纸质出版：2013
- Accepted：
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Application of restriction site amplified polymorphism (RSAP) to genetic diversity in Saccharina japonica[J]. 海洋湖沼学报（英文）, 2013,31(4):830-834.

Zhao, C., Liu, C., Li, W. et al. Application of restriction site amplified polymorphism (RSAP) to genetic diversity in Saccharina japonica, ., Chin. J. Ocean. Limnol. 31, 830–834 (2013). https://doi.org/10.1007/s00343-013-2122-2
Application of restriction site amplified polymorphism (RSAP) to genetic diversity in Saccharina japonica[J]. 海洋湖沼学报（英文）, 2013,31(4):830-834. DOI： 10.1007/s00343-013-2122-2.

Zhao, C., Liu, C., Li, W. et al. Application of restriction site amplified polymorphism (RSAP) to genetic diversity in Saccharina japonica, ., Chin. J. Ocean. Limnol. 31, 830–834 (2013). https://doi.org/10.1007/s00343-013-2122-2 DOI：

摘要

Abstract

Restriction site amplified polymorphism (RSAP) was used

for the first time

to analyze the genetic structure and diversity of four

mainly cultivated

varieties of the brown alga

Saccharina japonica. Eighty-eight samples from varieties “ Rongfu ”

“ Fujian ”

“ Ailunwan ” and “ Shengchanzhong ” were used for the genetic analyses. One hundred and ninety-eight bands were obtained using eight combinations of primers. One hundred and ninety-one (96.46%) were polymorphic bands. Nei’s genetic diversity was 0.360

and the coefficient of genetic differentiation was 0.357. No inbreeding-type recession was found in the four brown alga varieties and the results of the “ Ailunwan ” variety using samples from 2 years showed that the variety was becoming less diverse during the selection inherent in the breeding program. Genetic diversity and cluster analyses results were consistent with these genetic relationships. The results show the RSAP method is suitable for genetic analysis. Continuous inbreeding and selection could reduce the genetic diversity effectively; therefore periodical supervision is required.

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Keywords

references

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