Effects of nutrients and light intensity on the growth and biochemical composition of a marine microalga Odontella aurita

Song Xia; Linglin Wan; Aifen Li; Min Sang; Chengwu Zhang

doi:10.1007/s00343-013-2092-4

Your Location：

Home >

Browse articles >

Effects of nutrients and light intensity on the growth and biochemical composition of a marine microalga Odontella aurita

OriginalPaper | Updated：2023-05-16

- Effects of nutrients and light intensity on the growth and biochemical composition of a marine microalga Odontella aurita
- Effects of nutrients and light intensity on the growth and biochemical composition of a marine microalga Odontella aurita
- 海洋湖沼学报（英文） 2013年31卷第6期页码：1163-1173
- Affiliations：
  
  Institute of Hydrobiology Jinan University
- Author bio：
- Funds：
  
  Supported by the National High Technology Research and Development Program of China (863 Program) (Nos. 2009AA06440, 2013AA065805), the National Basic Research Program of China (973 Program) (No. 2011CB2009001), and the National Natural Science Foundation of China (No. 31170337)
- DOI：10.1007/s00343-013-2092-4
  中图分类号：
- 网络首发：2013-06-08，
  
  纸质出版：2013
- Accepted：
Scan QR Code
Effects of nutrients and light intensity on the growth and biochemical composition of a marine microalga Odontella aurita[J]. 海洋湖沼学报（英文）, 2013,31(6):1163-1173.

Xia, S., Wan, L., Li, A. et al. Effects of nutrients and light intensity on the growth and biochemical composition of a marine microalga Odontella aurita, ., Chin. J. Ocean. Limnol. 31, 1163–1173 (2013). https://doi.org/10.1007/s00343-013-2092-4
Effects of nutrients and light intensity on the growth and biochemical composition of a marine microalga Odontella aurita[J]. 海洋湖沼学报（英文）, 2013,31(6):1163-1173. DOI： 10.1007/s00343-013-2092-4.

Xia, S., Wan, L., Li, A. et al. Effects of nutrients and light intensity on the growth and biochemical composition of a marine microalga Odontella aurita, ., Chin. J. Ocean. Limnol. 31, 1163–1173 (2013). https://doi.org/10.1007/s00343-013-2092-4 DOI：

摘要

Abstract

Algal biotechnology has advanced greatly in the past three decades. Many microalgae are now cultivated to produce bioactive substances. Odontella aurita is a marine diatom industrially cultured in outdoor open ponds and used for human nutrition. For the first time

we have systematically investigated the effects of culture conditions in cylindrical glass columns and flat-plate photobioreactors

including nutrients (nitrogen

phosphorus

silicon

and sulfur)

light intensity and light path

on O. aurita cell growth and biochemical composition (protein

carbohydrate

β-1

3-glucan

lipids

and ash). The optimal medium for photoautotrophic cultivation of O. aurita contained 17.65 mmol/L nitrogen

1.09 mmol/L phosphorus

0.42 mmol/L silicon

and 24.51 mmol/L sulfur

yielding a maximum biomass production of 6.1–6.8 g/L and 6.7–7.8 g/L under low and high light

respectively. Scale-up experiments were conducted with flat-plate photobioreactors using different light-paths

indicating that a short light path was more suitable for biomass production of O. aurita. Analyses of biochemical composition showed that protein content decreased while carbohydrate (mainly composed of β-1

3-glucan) increased remarkably to about 50% of dry weight during the entire culture period. The highest lipid content (19.7% of dry weight) was obtained under 0.11 mmol/L silicon and high light conditions at harvest time. Fatty acid Profiles revealed that 80% were C14

C16

and C20

while arachidonic acid and eicosapentaenoic acid (EPA) accounted for 1.6%–5.6% and 9%–20% of total fatty acids

respectively. High biomass production and characteristic biochemical composition Profiles make O. aurita a promising microalga for the production of bioactive components

such as EPA and β-1

3-glucan.

关键词

Keywords

references

Basova M M. 2005. Fatty acid composition of lipid in microalgae. Int. J. Algae, 7: 33–57.

Brand J P. 1998. Simultaneous culture in pilot tanks of the macroalga Chondrus crispus and the microalgae Odontella aurita producing EPA. Actes Colloq. IFREMER, 21: 39–47.

Calder P C. 2012. The role of marine omega-3 (n -3) fatty acids in inflammatory processes, atherosclerosis and plaque stability. Mol. Nutr. Food. Res., 56: 1 073–1 080.

Carvalho A P, Monteiro C M, Malcata F X. 2009. Simultaneous effect of irradiance and temperature on biochemical composition of the microalga Pavlova lutheri. J. Appl. Phycol., 21: 543–552.

Chen Y C. 2012. The biomass and total lipid content and composition of twelve species of marine diatoms cultured under various environments. Food Chem., 131: 211–219.

Chihara M, Murano M. 1997. An Illustrated Guide to Marine Plankton in Japan. Tokai University Press, Tokyo.

Chu S P. 1943. The influence of the mineral composition of the medium on the growth of planktonic algae. II. The influence of the concentration of inorganic nitrogen and phosphate phosphorus. J. Ecol., 31: 109–148.

Cohen Z, Norman H A, Heimer Y M. 1993. Potential use of substituted pyridazinones for selecting polyunsaturated fatty acid overproduing cell lines of algae. Phytochemitry, 32: 259–264.

Del Rio E, Acien G, Garcia-Malea M C et al. 2005. Efficient one-step production of astaxanthin by the microalga Haematococcus pluvialis in continuous culture. Biotechnol. Bioeng., 91: 808–815.

Drebes D, Schnepf E. 1998. Gyrodinium undulans Hulburt, a marine dinoflagellate feeding on the bloom-forming diatom Odontella aurita, and on copepod and rotifer eggs. Helgolander Meeresunters, 52: 1–14.

Droop M R. 1975. The nutrient status of algal cell in batch culture. J. Mar. Biol. Assoc. UK, 55: 541–555.

Dubios M, Gillies K A, Hamilton J K et al. 1956. Colorimetric method for the determination of sugars and related subtacnces. Anal. Chem., 28: 350–356.

Granum E, Myklestad S M. 2002. A simple combined method for determination of β-1, 3-glucan and cell wall polysaccharides in diatoms. Hydrobiologia, 477: 155–161.

Guerrini F, Cangini M, Boni L. 2000. Metabolic responses of the diatom Achnanthes brevipes (Bacillariophyceae) to nutrient limitation. J. Phycol., 36: 882–890.

Guiheneuf F, Fouqueray M, Mimouni V et al. 2010. Effect of UV stress on the fatty acid and lipid class composition in two marine microalgae Pavlova lutheri (Pavlovophyceae) and Odontella aurita (Bacillariophyceae). J. Appl. Phycol., 22: 629–638.

Guillard R R L, Hargraves P E. 1993. Stichochrysis immobilis is a diatom, not a chrysophyte. Phycologia, 32: 234–236.

Hemaiswarya S, Raja R, Kumar R R et al. 2011. Microalgae: a sustainable feed source for aquaculture. World J. Microbiol. Biotechnol., 27: 1 737–1 746.

Hu Q, Sommerfeld M, Jarvis E et al. 2008. Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J., 54: 621–639.

Khozin-Goldberg I, Shrestha P, Cohen Z. 2005. Mobilization of arachidonyl moieties from triacylglycerols into chloroplastic lipids following recovery from nitrogen starvation of the microalga Parietochloris incisa. J. Biochem. Biophy. Acta, 1738: 63–71.

Kobayashi K, Fujita K. 1997. Tube diameter on tubular photobioreactor for microalgal culture and its biomass productivity. J. Chem. Eng. Jpn., 30: 339–341.

Liang Y, Mai K, Sun S. 2005. Differences in growth, total lipid content and fatty acid composition among 60 clones of Cylindrotheca fusiformis. J. Appl. Phycol., 17: 61–65.

Lourenço S, Barbarino E, Lavín P et al. 2004. Distribution of intracellular nitrogen in marine microalgae: Calculation of new nitrogen-to-protein conversion factors. Eur. J. Phycol., 39: 17–32.

Lynn S G, Kilham S S, Kreeger D A et al. 2000. Effect of nutrient availability on the biochemical and elemental stoichiometry in freshwater diatom Stephanodiscus minutulus (Bacillariophyceae). J. Phycol., 36: 510–522.

Meiser A, Staiger U S, Trosch W. 2004. Optimization of eicosapentaenoic acid production by Phaeodactylum tricornutum in the flat panel airlift (FPA) reactor. J. Appl. Phycol., 16: 215–225.

Moreau D, Tomasoni C, Jacquot C et al. 2006. Cultivated microalgae and the carotenoid fucoxanthin from Odontella aurita as potent anti-proliferative agents in bronchopulmonary and epithelial cell lines. Environ. Toxicol. Pharmacol., 22: 97–103.

Myklestad S, Haug A. 1972. Production of carbohydrates by the marine diatiom Chaetoceros affinis var. Willei (Gran) Hustedt. I. Effect of the concentration of nutrients in the culture medium. J. Exp. Mar. Biol. Ecol., 9: 125–136.

Otsuka H. 1961. Changes of lipid and carbohydrate contents in Chlorella cells during the sulfur starvation, as studied by the technique of synchronous culture. J. Gen. Appl. Microbiol., 7: 72–77.

Pahl S L, Lewis D M, Chen F et al. 2010. Growth dynamics and the proximate biochemical composition and fatty acid Profile of the heterotrophically grow diatom Cyclotella cryptica. J. Appl. Phycol., 22: 165–171.

Paulsen B S, Myklestad S. 1978. Structural studies of the reserve glucan produced by the marine diatom Skeletonema costatum (Grev.) Cleve. Carbohydr. Res., 62: 386–388.

Qian K X, Borowitzka M A. 1993. Light and nitrogen deficiency effects on the growth and composition of Phaeodactylum tricornutum. Appl. Biochem. Biotechnol., 38: 93–103.

Raja R, Hemaiswarya S, Rengasamy R. 2007. Exploitation of Dunaliella for β-carotene production. Appl. Microbiol. Biotechnol., 74: 517–523.

Reitan K I, Rainuzzo J R, Olscn Y. 1994. Effect of nutrient limitation on fatty acid and lipid content of marine microalgae. J. Phycol., 30: 972–979.

Roessler P G. 1988. Changes in the activities of various lipid and carbohydrate biosynthetic enzymes in the diatom Cyclotella cryptica in response to silicon deficiency. Arch Biochem. Biophys., 267: 521–528.

Roessler P G. 1990. Environmental control of glycerolipid metabolism in microalgae: commercial implications and future research directions. J. Phycol., 26: 393–399.

Sarada D V L, Kumar C S, Rengasamy R. 2011. Purified C-phycocyanin from Spirulina platensis (Nordstedt) Geitler: a novel and potent agent against drug resistant bacteria. World J. Microbiol. Biotechnol., 27: 779–783.

Spoehr H A, Milner H W. 1949. The chemical composition of Chlorella: effect of environmental conditions. Plant Physiol., 24: 120–149.

Storseth T R, Hansen K, Skjermo J et al. 2004. Characterization of a β-D-(1→3)-glucan from the marine diatom Chaetoceros mulleri by high-resolution magic-angle spinning NMR spectroscopy on whole algal cells. Carbohydr. Res., 339: 421–424.

Sukenik A, Livne A. 1991. Variations in lipid and fatty acid content in relation to acetyl CoA carboxylase in the marine Prymnesiophyte Isochrysis galbana. Plant Cell Physiolol., 32: 371–378.

Toume K, Ishibashi. 2002. 5α, 8α-Epidioxysterol sulfate from a diatom Odontella aurita. Phytochemistry, 61: 359–360.

Yeh K L, Chang J S. 2012. Effects of cultivation conditions and media composition on cell growth and lipid productivity of indigenous microalga Chlorella vulgaris ESP-31. Bioresource Technol., 105: 120–127.

Yongmanitchai W, Ward O P. 1991. Growth of and Omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions. Appl. Enviro. Microbiol., 57: 419–425.

Zhang C W, Cohen Z, Goldberg I K et al. 2002. Characterization of growth and arachidonic acid production of Parietochloris incise comb. Nov (Trebouxiophyceae, Chlorophyta). J. Appl. Phycol., 14: 453–460.

Zhang C W, Richmond A. 2003. Sustainable, high-yielding outdoor mass cultures of Chaetoceros muelleri var. subsalsum and Isochrysis galbana in vertical plate reactors. Mar. Biotechnol., 5: 302–310.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Experimental study on interaction between epizoic diatoms and Pseudodiaptomus annandalei

Succession ofAphanizomenon flos-aquaeandMicrocystis aeruginosain direct co-culture experiments at different temperatures and biomasses

Isolation of a novel strain of Cyanobacterium sp. with good adaptation to extreme alkalinity and high polysaccharide yield

The vertical structure of the vegetative canopy of the brown algae Cystoseira (Black Sea)

Spatial and seasonal distribution of intertidal macrobenthos with their biomass and functional feeding guilds in the Naf River estuary, Bangladesh