Development of an in situ loop-mediated isothermal amplification technique for chromosomal localization of DNA sequences

Qinglei Meng; Shi Wang; Lingling Zhang; Xiaoting Huang; Zhenmin Bao

doi:10.1007/s00343-013-2070-x

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Development of an in situ loop-mediated isothermal amplification technique for chromosomal localization of DNA sequences

OriginalPaper | Updated：2023-05-16

- Development of an in situ loop-mediated isothermal amplification technique for chromosomal localization of DNA sequences
- Development of an in situ loop-mediated isothermal amplification technique for chromosomal localization of DNA sequences
- 海洋湖沼学报（英文） 2013年31卷第1期页码：128-133
- Affiliations：
  
  1. Key Laboratory of Marine Genetics and Breeding (MGB) Ministry of Education College of Marine Life Sciences Ocean University of China
  2. Shandong Provincial Key Laboratory of Freshwater Genetics and Breeding Freshwater Fisheries Research Institute of Shandong Province
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (Nos. 31270047, 30901096), the National Key Technology R&D Program of China (Nos. 2011BAD45B01, 2011BAD13B05), and the Earmarked Fund For Modern Agro-Industry Technology Research System to Dr. Zhenmin BAO
- DOI：10.1007/s00343-013-2070-x
  中图分类号：
- 网络首发：2013-01-16，
  
  纸质出版：2013
- Accepted：
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Development of an in situ loop-mediated isothermal amplification technique for chromosomal localization of DNA sequences[J]. 海洋湖沼学报（英文）, 2013,31(1):128-133.

Meng, Q., Wang, S., Zhang, L. et al. Development of an in situ loop-mediated isothermal amplification technique for chromosomal localization of DNA sequences., Chin. J. Ocean. Limnol. 31, 128–133 (2013). https://doi.org/10.1007/s00343-013-2070-x
Development of an in situ loop-mediated isothermal amplification technique for chromosomal localization of DNA sequences[J]. 海洋湖沼学报（英文）, 2013,31(1):128-133. DOI： 10.1007/s00343-013-2070-x.

Meng, Q., Wang, S., Zhang, L. et al. Development of an in situ loop-mediated isothermal amplification technique for chromosomal localization of DNA sequences., Chin. J. Ocean. Limnol. 31, 128–133 (2013). https://doi.org/10.1007/s00343-013-2070-x DOI：

摘要

Abstract

In situ loop-mediated isothermal amplification (in situ LAMP) combines in situ hybridization and loop-mediated isothermal amplification (LAMP) techniques for chromosomal localization of DNA sequences. In situ LAMP is a method that is generally more specific and sensitive than conventional techniques such as fluorescence in situ hybridization (FISH)

primed in situ labeling (PRINS)

and cycling primed in situ labeling (C-PRINS). Here

we describe the development and application of in situ LAMP to identify the chromosomal localization of DNA sequences. To benchmark this technique

we successfully applied this technique to localize the major ribosomal RNA gene on the chromosomes of the Zhikong scallop (Chlamys farreri).

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references

Gosden J, Hanratty D. 1993. PCR in situ: a rapid alternative to in situ hybridization for mapping short, low-copy number sequences without isotopes. Biotechniques, 15: 78–83.

Harrer T, Schwinger E, Mennicke K. 2001. A new technique for cyclic in situ amplification and a case report about amplification of a single copy gene sequence in human metaphase chromosomes through PCR-PRINS. Hum. Mutat., 17: 131–140.

Huang X T, Bao Z M, Bi K, Hu J J, Zhang C, Zhang Q Q, Hu X L. 2006. Chromosomal localization of the major ribosomal RNA genes in scallop Chlamys farreri. Acta Oceanol. Sin., 25: 108–115.

Koch J, Kolvraa S, Peterson K B, Gregersen N, Bolund L. 1989. Oligonnucleotide-priming methods for the chromosome-specific labeling of alpha satellite DNA in situ. Chromosoma, 98: 259–265.

Koch J. 1996. Primed in situ labeling as a fast and sensitive method for the detection of specific DNA sequences in chromosomes and nuclei. Method, 9: 122–128.

Kubalakova M, Vrana J, Cihalikova J, Lysak M A, Dolezel J. 2001. Localisation of DNA sequences on plant chromosomes using PRINS and C-PRINS. Methods Cell Sci., 23: 71–82.

Mukai Y, Appels R. 1996. Direct chromosome mapping of plant genes by in situ polymerase chain reaction (in situ PCR). Chromosome Res., 4: 401–403.

Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T. 2000. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res., 28: e63.

Pellestor F, Girardet A, Andreo B, Charlieu J P. 1994. A polymorphic alpha satellite sequence specific for human chromosome 13 detected by oligonucleotide primed in situ labeling (PRINS). Hum. Genet., 94: 346–348.

Pellestor F, Girardet A, Lefort G, Andreo B, Charlieu J P. 1995. Use of the primed in situ labeling (PRINS) technique for a rapid detection of chromosomes 13, 16, 18, 21, X and Y. Hum. Genet., 95: 12–17.

Sambrook J, Fritsch E F, Maniatis T. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York.

Speel E J M, Ramaekers F C S, Hopman A H N. 2003. PCR-based detection of nucleic acids in chromosomes, cells, and tissues. Methods Mol. Biol., 226: 405–424.

Trask B J. 1991. DNA sequence localization in metaphase and interphase cells by fluorescence in situ hybridization. Methods Cell Biol., 35: 3–35.

Wang Y P, Guo X M. 2004. Chromosomal rearrangement in Pectinidae revealed by rRNA loci and implications for bivalve evolution. Biol. Bull., 207: 247–256.

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