Screening of marine fungus from Nanji Island and activity of their metabolites against pathogenic Vibrio from Pseudosciaena crocea

Shujiang Zhao; Shuping Li; Huihui Liu; Qian Zhao; Jieyou Wang; Maocang Yan

doi:10.1007/s00343-012-2003-0

Your Location：

Home >

Browse articles >

Screening of marine fungus from Nanji Island and activity of their metabolites against pathogenic Vibrio from Pseudosciaena crocea

OriginalPaper | Updated：2023-05-16

- Screening of marine fungus from Nanji Island and activity of their metabolites against pathogenic Vibrio from Pseudosciaena crocea
- Screening of marine fungus from Nanji Island and activity of their metabolites against pathogenic Vibrio from Pseudosciaena crocea
- 海洋湖沼学报（英文） 2012年30卷第5期页码：746-756
- Affiliations：
  
  1. School of Environmental Science and Public Health Wenzhou Medical College
  2. Department of Pharmacy Zhengzhou Huaxin College
  3. Mariculture Institute of Zhejiang
- Author bio：
- Funds：
  
  Supported by the General Program of Zhejiang Science and Technology Department (No. 2009C33004)
- DOI：10.1007/s00343-012-2003-0
  中图分类号：
- 网络首发：2012-05-27，
  
  纸质出版：2012
- Accepted：
Scan QR Code
Screening of marine fungus from Nanji Island and activity of their metabolites against pathogenic Vibrio from Pseudosciaena crocea[J]. 海洋湖沼学报（英文）, 2012,30(5):746-756.

Zhao, S., Li, S., Liu, H. et al. Screening of marine fungus from Nanji Island and activity of their metabolites against pathogenic Vibrio from Pseudosciaena crocea, ., Chin. J. Ocean. Limnol. 30, 746–756 (2012). https://doi.org/10.1007/s00343-012-2003-0
Screening of marine fungus from Nanji Island and activity of their metabolites against pathogenic Vibrio from Pseudosciaena crocea[J]. 海洋湖沼学报（英文）, 2012,30(5):746-756. DOI： 10.1007/s00343-012-2003-0.

Zhao, S., Li, S., Liu, H. et al. Screening of marine fungus from Nanji Island and activity of their metabolites against pathogenic Vibrio from Pseudosciaena crocea, ., Chin. J. Ocean. Limnol. 30, 746–756 (2012). https://doi.org/10.1007/s00343-012-2003-0 DOI：

摘要

Abstract

Seventy-eight marine fungal strains were isolated from sediment samples collected off the coast of Nanji Island

Wenzhou

Zhejiang Province

China. Antibacterial screening using the agar disc method showed that 19 of the isolated strains could inhibit at least one pathogenic V ibrio from P seudosciaena crocea. Subsequent screening confirmed that nine strains produced antibacterial metabolites that had activity against one or several types of pathogenic V ibrio. Strain NJ0104 had the widest antimicrobial spectrum and strong activity

particularly against Vibrio parahaemolyticus-MM0810072. A preliminary study of NJ0104 antibacterial metabolites demonstrated that they had thermal stability up to 80°C

ultraviolet stability up to 40 min and pH stability between 4.0–7.0. In addition

the antibacterial metabolites were readily soluble in butanol. To identify the specific strain

the ITS-5.8S rDNA regions of NJ0104 were PCR amplified and sequenced. Based on the combination of phenotypic and genotypic data

the strain was identified as Arthrinium sp.

关键词

Keywords

references

Accensi F, Cano J, Figueira L, Abarca M L, Cabañes F J. 1999. New PCR method to differentiate species in the Aspergillus niger aggregate. FEMS Microb. Lett., 180: 191–196.

Bao H Z, Kong D P, Wang Z C, Ma G Z, Xiao Y. 2009. Isolation of marine bacteria against V ibrio and detection of antimicrobial activity. Fish. Sci., 28(1): 24–27. (in Chinese with English abstract)

Bao S X, Huang H Q, 2008. Marine Microbiology. China Ocean University Press, Qingdao. p.166–172. (in Chinese)

Bugni T S, Ireland C M. 2004. Marine-derived fungi: a chemically and biologically diverse group of microorganisms. Nat. Pro. Rep., 21: 143–163.

Christophersen C, Crescente O, Frisvad J C, Gram L, Nielsen J, Nielsen P H, Rahbak L. 1999. Antibacterial activity of marine-derived fungi. Mycopath., 143(3): 135–138.

Feling R H, Buchanan G O, Mincer T J, Kaufman C A, Jensen P R, Fenical W. 2003. Salinosporamide A: a highly cytotoxic proteasome inhibitor from a novel microbial source, a marine bacterium of the new genus Salinospora. Angew. Chem. Intern. Edit., 42(3): 355–357.

Fusetani N. 2000. Introduction. In: Fusetani N ed. Drugs from the Sea. Karger A G, Basel. p.1–5.

Gao A G, Zeng J N, Chen Q Z et al. 2007. Time and space distribution of Molluscas of intertidal zone in Nanji Archipalego Marine Nature Reserve. Acta Ocean. Sin., 29(2): 105–111.

Guo J, Zu G R, Kong F D, Han D, Yang D. 2007. Fermentation conditions of antibacterial substance by a marine fungal strain M-401. J. Microb., 27(1): 76–77. (in Chinese with English abstract)

Hao S Z, Wang S Y, Wang L. 2006. Screening and identification of a marine mildew for inhibiting pathogenic fungi of plants. Subtrop. Agri. Res., 2(1): 45–48. (in Chinese with English abstract)

Hao G J, Shen J Y, Xu Y, Yao J Y, Pan X Y, Yin W L. 2009. Preparation and applications of monoclonal antibody against the pathogenic harveyi from Pseudosciaena crocea. Acta hydrob. Sin., 33(3): 413–417. (in Chinese with English abstract)

Haug I. 2002. Identification of Picea-ectomycorrhizae by comparing DNA-sequences. Mycol. Prog., 1(2): 167–178.

Huang S S, Xu L L, Huang X, Zhang R C, Chen L M, Lei F, Li K Z. 2010. Isolation and identification of 3 strains of marine fungi with antagonistic ability against litchi and rice pathogenic fungi. Genom. Appl. Biol., 29(4): 665–672. (in Chinese with English abstract)

Huang Y J, Chen L X, Zheng Z H, Chen P J, Su W J. 1998. Distribution of symbiotic and epiphyte antibiotic microorganisms intertidal zone of Xiamen Sea Area. Mar. Sci. Bull., 17(2): 37–41. (in Chinese with English abstract)

Ji H H, Ye S F, Liu X, Hong J C. 2006. The species composition and diversity of zooplankton in Nanji Islands National Nature Reserve. Biodiv. Sci., 14(3): 206–215. (in Chinese with English abstract)

Khudyakova Y V, Pivkin M V, Kuznetsova T A, Svetashev V I. 2000. Fungi in sediments of the sea of Japan and their biologically active metabolites. Microb., 69(5): 608–611.

Li X, Yao Y H, Zhang Y N, Lin W H, Isabel S. 2007. Structure elucidation of actinopyrone A and C isolated from marine fungus Penicillium sp. Chin. J. Magnet. Reson., 24(3): 275–280.

Liu S, Lu Z X, Lü F X, Fang X W, Ding Z Y. 2007. The identification and antimicrobial activity of a marine actinomycete. J. Nanjing Agri. Univ., 30(4): 124–129. (in Chinese with English abstract)

Ma A Y. 2010. Application of rDNA analysis in the classification and identification of fungus. J. Anhui Agric. Sci., 38(32): 18 079–18 081. (in Chinese with English abstract)

Ma Y X, Sun T, Wang H Y, Wang Y, Zhu Y. 2004. Antimicrobial activity in the epiphytic fungi on sea weed species in intertidal zone of Dalian. J. Dalian Fish. Univ., 19(1): 6–9. (in Chinese with English abstract)

Magnani M, Fernandes T, Prete C E C, Homechim M, Ono E Y S, Vilas-Boas L A, Sartori D, Furlaneto M C, Fungaro M H P. 2005. Molecular identification of Aspergillus species isolated from coffee beans. Sci. Agric. 62(1): 45–49.

Meng Q H, Zhang H, Zhang J, 2007. Isolation and identification of marine fungi from Lüju River in Bohai Sea. J. Tianjin Norm. Univ. (Nat. Sci. Ed.), 27(1): 28–31. (in Chinese with English abstract)

Miao L, Kwong T F N, Qian P Y. 2006. Effect of culture conditions on mycelial growth, antibacterial activity, and metabolite profiles of the marine-derived fungus Arthrinium c.f. saccharicola. Appl. Microb. Biotech., 72(5): 1 063–1 073.

Morillo J A, Aguilera M, Antízar-Ladislao B, Fuentes S, Ramos-Cormenzana A, Russell N J, Monteoliva-Sánchez M. 2008. Molecular microbial and chemical investigation of the bioremediation of two-phase olive mill waste using laboratory-scale bioreactors. Appl. Microb. Biotech., 79(2): 309–317.

Peng C, Zheng Z H, Du X P, Huang Y J, Song S Y, Su W J. 2005. Effects of different kinds of media on the growth and active metabolite production of algicolous marine fungus PT2. J. Oceanogr. In Taiwan Strait, 24(1): 90–96. (in Chinese with English abstract)

Qi S H, Xu Y, Xiong H R, Qian P Y, Zhang S. 2009. Antifouling and antibacterial compounds from a marine fungus Cladosporium sp. F14. World J. Microb. Biotech., 25(3): 399–406.

Renske L, Paula L, Thom W K, Ellis H, Anna R, Karel W, Eric S. 2003. Molecular identification of ectomycorrhizal mycelium in soil horizons. Appl. Environm. Microb., 69(1): 327–333.

Sheng P, Fan X R, Li G W. 1999. Microbial Experiment. Higher Education Press, Beijing. p.1–234. (in Chinese)

Shi J X, Chen Z Y, Hu X G. 1994. Abundance of bacteria and its activity in the waters nearby the Nanji Islands. Donghai Mar. Sci., 12(3): 57–61. (in Chinese with English abstract)

Su Y Q, Zhu H W, Ma X H, Ma Y M, Sun K. 2005. Screening of antibacterial activities of endophytic fungi isolated from Eucommia ulmoides. Acta Bot. Boreal Occident. Sin., 25(6): 1 153–1 157. (in Chinese with English abstract)

Tong G Z, Shi S Y, Xue C B. 2005. Analysis of pathogenic bacteria and drug sensitivity test of the tail-rot Pseudosciaena crocea cultured in marine cage in Zhoushan. J. Zhejiang Ocean Univ. (Nat. Sci. Ed.), 24(2): 118–121. (in Chinese with English abstract)

Wang X Y, Huang Y J, Zheng Z H, Gao Y H, Song S Y. 2006. Study on benthal fungi from sediments of Zhoushan archipelago and anitibiosis screen. J. Xiamen Univ. (Nat. Sci. Ed.), 45(4): 558–562. (in Chinese with English abstract)

Wang S J, Hu J C, Xue D L, Ma C X, Xie Q H, Liu Q Y. 2001. Study of marine microbial resources in Huanghai, Bohai and Liaoning offshore of China. J. Jinzhou Norm. Coll., 22(1): 1–5. (in Chinese with English abstract)

Wu W T, Wang Y T, Wu W J. 2000. Advances of several Study on marine active substances. Pharm. Biotech., 7(3): 179–183. (in Chinese with English abstract)

Xiong F, Zheng Z H, Huang Y J, Song S Y, Xu Q Y, Su W J. 2006. Antimicrobial and antitumor activities of marine fungi isolated from sediment in deep sea. J. Xiamen Univ. (Nat. Sci. Ed.), 45(3): 419–423. (in Chinese with English abstract)

Yan X F, Huang D H, Dai Y B, Zhang L R. 2007. Study on antioxidant activity of marine fungi. J. Xiamen Univ. (Nat. Sci. Ed.), 46(3): 418–421. (in Chinese with English abstract)

Yang Y H, Xu Z N, Lin X T, Ma Y S. 2008. Culture screening for waters and sediment fungi growing in artificial lake. J. Jinan Univ. (Nat. Sci. Ed.), 29(5): 510–515. (in Chinese with English abstract)

Zhang C B, Su X Q. 2006. Advance of research on several technologies for fungi identification based on genome DNA. J. Guizhou Norm. Univ. (Nat. Sci. Ed.), 24(1): 113–118. (in Chinese with English abstract)

Zhou R L, Mu J, Zhang Y, Xie Z P. 2008. Recent progress in the research on bioactive substances from marine fungi. Nat. Prod. Res. Dev., 20: 741–747. (in Chinese with English abstract)

Zhu F, Chen G Y, Lin Y C. 2007. Structural determination of three sphingolipids from two marine-derived mangrove endophytic fungal strains. Mar. Sci. Bull., 9(1): 20–25. (inChinese with English abstract)

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

β-cyclopiazonic acid binds iron demonstrating siderophore-like activity and promotes growth in Pseudomonas aeruginosa

On multiple solutions to a low-spectrum model of oceanic current driven by the boundary force—The bimodality of the Kuroshio south of Japan

Determination of sedimentary rate in estuary and coast by228Th technique

Purification and characterization of cold-active endo-1,4-β-glucanase produced by Pseudoalteromonas sp. AN545 from Antarctica

V形前缘对激波入射边界层流动影响的数值模拟与分析