Extract of Meretrix meretrix Linnaeus induces angiogenesis in vitro and activates endothelial nitric oxide synthase

Ming Liu; Jianteng Wei; Hui Wang; Lili Ding; Yuyan Zhang; Xiukun Lin

doi:10.1007/s00343-012-1241-5

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Extract of Meretrix meretrix Linnaeus induces angiogenesis in vitro and activates endothelial nitric oxide synthase

OriginalPaper | Updated：2023-05-16

- Extract of Meretrix meretrix Linnaeus induces angiogenesis in vitro and activates endothelial nitric oxide synthase
- Extract of Meretrix meretrix Linnaeus induces angiogenesis in vitro and activates endothelial nitric oxide synthase
- 海洋湖沼学报（英文） 2012年30卷第5期页码：724-730
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
  3. Department of Pharmacology Capital Medical University
- Author bio：
- Funds：
  
  Supported by the Innovative Drug Development Projects of China (Nos. 2009ZX09103-661 and 2009ZX09102) and the National Natural Science Foundation of China (No. 81001396)
- DOI：10.1007/s00343-012-1241-5
  中图分类号：
- 网络首发：2012-09-05，
  
  纸质出版：2012
- Accepted：
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Extract of Meretrix meretrix Linnaeus induces angiogenesis in vitro and activates endothelial nitric oxide synthase[J]. 海洋湖沼学报（英文）, 2012,30(5):724-730.

Liu, M., Wei, J., Wang, H. et al. Extract of Meretrix meretrix Linnaeus induces angiogenesis in vitro and activates endothelial nitric oxide synthase., Chin. J. Ocean. Limnol. 30, 724–730 (2012). https://doi.org/10.1007/s00343-012-1241-5
Extract of Meretrix meretrix Linnaeus induces angiogenesis in vitro and activates endothelial nitric oxide synthase[J]. 海洋湖沼学报（英文）, 2012,30(5):724-730. DOI： 10.1007/s00343-012-1241-5.

Liu, M., Wei, J., Wang, H. et al. Extract of Meretrix meretrix Linnaeus induces angiogenesis in vitro and activates endothelial nitric oxide synthase., Chin. J. Ocean. Limnol. 30, 724–730 (2012). https://doi.org/10.1007/s00343-012-1241-5 DOI：

摘要

Abstract

Meretrix meretrix Linnaeus has long been used as traditional Chinese medicine in oriental medicine. The angiogentic activity of the extract of M. meretrix was investigated in this study

using human umbilical vein endothelial cells (HUVECs). Extract of M. meretrix Linnaeus (AFG-25) was prepared with acetone and ethanol precipitation

and further separated by Sephadex G-25 column. The results show that AFG-25 promoted proliferation

migration

and capillary-like tube formation in HUVECs

and in the presence of eNOS inhibitor NMA

the tube formation induced by AFG-25 is inhibited significantly. Moreover

AFG-25 could also promote the activation of endothelial nitric oxide synthase (eNOS) and the resultant elevation of nitric oxide (NO) production. The results suggested that M. meretrix contains active ingredients with angiogentic activity and eNOS/NO signal pathway is in part involved in the proangiogenesis effect induced by AFG-25.

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references

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