Tissue-specific bioaccumulation and oxidative stress responses in juvenile Japanese flounder (Paralichthys olivaceus) exposed to mercury

Wei Huang; Liang Cao; Zhenjiang Ye; Longshan Lin; Quanzhen Chen; Shuozeng Dou

doi:10.1007/s00343-012-1210-z

Your Location：

Home >

Browse articles >

Tissue-specific bioaccumulation and oxidative stress responses in juvenile Japanese flounder (Paralichthys olivaceus) exposed to mercury

OriginalPaper | Updated：2023-05-16

- Tissue-specific bioaccumulation and oxidative stress responses in juvenile Japanese flounder (Paralichthys olivaceus) exposed to mercury
- Tissue-specific bioaccumulation and oxidative stress responses in juvenile Japanese flounder (Paralichthys olivaceus) exposed to mercury
- 海洋湖沼学报（英文） 2012年30卷第4期页码：569-579
- Affiliations：
  
  1. Laboratory of Marine Ecosystem and Biogeochemistry Second Institute of Oceanography State Oceanic Administration
  2. Key Laboratory of Marine Ecology and Environmental Sciences Institute of Oceanology Chinese Academy of Sciences
  3. Fishery College Ocean University of China
  4. Third Institute of Oceanography State Oceanic Administration
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China for Creative Research Groups (No. 41121064), the National High Technology Research and Development Program of China (863 Program) (No. 2009AA09Z401), the Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-YW-Q07-02), and the Public Interest Research Program in Marine Industry from the State Oceanic Administration of China (No. 200905019)
- DOI：10.1007/s00343-012-1210-z
  中图分类号：
- 网络首发：2012-07-02，
  
  纸质出版：2012
- Accepted：
Scan QR Code
Tissue-specific bioaccumulation and oxidative stress responses in juvenile Japanese flounder (Paralichthys olivaceus) exposed to mercury[J]. 海洋湖沼学报（英文）, 2012,30(4):569-579.

Huang, W., Cao, L., Ye, Z. et al. Tissue-specific bioaccumulation and oxidative stress responses in juvenile Japanese flounder (Paralichthys olivaceus) exposed to mercury., Chin. J. Ocean. Limnol. 30, 569–579 (2012). https://doi.org/10.1007/s00343-012-1210-z
Tissue-specific bioaccumulation and oxidative stress responses in juvenile Japanese flounder (Paralichthys olivaceus) exposed to mercury[J]. 海洋湖沼学报（英文）, 2012,30(4):569-579. DOI： 10.1007/s00343-012-1210-z.

Huang, W., Cao, L., Ye, Z. et al. Tissue-specific bioaccumulation and oxidative stress responses in juvenile Japanese flounder (Paralichthys olivaceus) exposed to mercury., Chin. J. Ocean. Limnol. 30, 569–579 (2012). https://doi.org/10.1007/s00343-012-1210-z DOI：

摘要

Abstract

To understand mercury (Hg) toxicity in marine fish

we measured Hg accumulation in juvenile Japanese flounder (Paralichthys olivaceus) and assessed the effects on growth and antioxidant responses. After Hg exposure (control

and 160 μg/L Hg) for 28 d

fish growth was significantly reduced. The accumulation of Hg in fish was dose-dependent and tissue-specific

with the maximum accumulation in kidney and liver

followed by gills

bone

and muscle. Different antioxidants responded differently to Hg exposure to cope with the induction of lipid peroxidation (LPO)

which was also tissue-specific and dosedependent. As Hg concentration increased

superoxide dismutase (SOD) and catalase (CAT) activities increased significantly

whereas glutathione S -transferase (GST) activity and glutathione (GSH) levels decreased significantly in the gills. SOD and glutathione peroxidase (GPx) activities and the GSH level increased significantly in the liver. SOD activity and GSH levels increased significantly

but CAT activity decreased significantly with an increase in Hg concentration in the kidney. LPO was induced significantly by elevated Hg in the gills and kidney but was least affected in the liver. Therefore

oxidative stress biomarkers in gills were more sensitive than those in the liver and kidney to Hg exposure. Thus

the gills have potential as bioindicators for evaluating Hg toxicity in juvenile flounder.

关键词

Keywords

references

Alvarez M D, Murphy C A, Rose K A, McCarthy I D, Fuiman L A. 2006. Maternal body burdens of methylmercury impair survival skills of offspring in Atlantic croaker (Micropogonias undulatus). Aquat. Toxicol., 80: 329–337 http://dx.doi.org/10.1016/j.aquatox.2006.09.010.

Atli G, Alptekin O, Tükel S, Canli M. 2006. Response of catalase activity to Ag+, Cd2+, Cr6+, Cu2+ and Zn2+ in five tissues of freshwater fish Oreochromis niloticus. Comp. Biochem. Physiol. C Toxicol. Pharmacol., 143: 218–224 http://dx.doi.org/10.1016/j.cbpc.2006.02.003.

Beers R F, Seizer I W. 1952. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J. Biol. Chem., 195: 133–140.

Berntssen M H G, Aatland A, Handy R D. 2003. Chronic dietary mercury exposure causes oxidative stress, brain lesions, and altered behaviour in Atlantic salmon (Salmo salar) parr. Aquat. Toxicol., 65: 55–72 http://dx.doi.org/10.1016/S0166-445X (03)00104-8.

Bradford M M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248–254.

Carta P, Flore C, Alinovi R, Ibba A, Tocco M G, Aru G, Carta R, Girei E, Mutti A, Lucchini R, Randaccio F S. 2003. Sub-clinical neurobehavioral abnormalities associated with low level of mercury exposure through fish consumption. Neurotoxicology, 24: 617–623, http://dx.doi.org/10.1016/S0161-813X(03)00080-9.

Chatterjee S, Bhattacharya S. 1984. Detoxication of industrial pollutants by the glutathione-S-transferase system in the liver of Anabas testudineus (Bloch). Toxicol. Lett., 22: 187–198.

Chen C Y, Dionne M, Mayes B M, Ward D M, Sturup S, Jackson B P. 2009. Mercury bioavailability and bioaccumulation in estuarine food webs in the Gulf of Maine. Environ. Sci. Technol., 43: 1 804–1 810, http://dx.doi.org/10.1021/es8017122.

Di Giulio R T, Washburn P C, Wenning R J, Winston G W, Jewell C S. 1989. Biochemical responses in aquatic animals: a review of determinants of oxidative stress. Environ. Toxicol. Chem., 8: 1 103–1 123, http://dx.doi.org/10.1897/1552-8618(1989)8[1103:BRIAAA]2.0.CO;2.

Elia A C, Galarini R, Taticchi M I, Dörr A J M, Mantilacci L. 2003. Antioxidant responses and bioaccumulation in Ictalurus melas under mercury exposure. Ecotoxicol. Environ. Saf., 55: 162–167, http://dx.doi.org/10.1016/S0147-6513(02)00123-9.

Faria M, Carrasco L, Diez S, Riva M C, Bayona J M, Barata C. 2009. Multi-biomarker responses in the freshwater mussel Dreissena polymorpha exposed to polychlorobiphenyls and metals. Comp. Biochem. Physiol. C Toxicol. Pharmacol., 149: 281–288 http://dx.doi.org/10.1016/j. cbpc.2008.07.012

Friedmann A S, Watzin M C, Brinck-Johnsen T, Leiter J C. 1996. Low levels of dietary methylmercury inhibit growth and gonadal development in juvenile walleye (Stizostedion vitreum). Aquat. Toxicol., 35: 265–278, http://dx.doi.org/10.1016/0166-445X(96)00796-5.

Gonzalez P, Dominique Y, Massabuau J C, Boudou A, Bourdineaud J P. 2005. Comparative effects of dietary methylmercury on gene expression in liver, skeletal muscle and brain of the zebra fish (Danio rerio). Environ. Sci. Technol., 39: 3 972–3 980, http://dx.doi.org/10.1021/es0483490.

Guilherme S, Válega M, Pereira M E, Santos M A, Pacheco M. 2008. Antioxidant and biotransformation responses in Liza aurata under environmental mercury exposure-Relationship with mercury accumulation and implications for public health. Mar. Pollut. Bull., 56: 845–859 http://dx.doi.org/10.1016/j.marpolbul.2008.02.003.

Habig W H, Pabst M J, Jakoby W B. 1974. Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. J. Biol. Chem., 249: 7 130–7 139.

Hissin P J, Hilf R. 1976. A fluorometric method for determination of oxidized and reduced glutathione in tissues. Anal. Biochem., 74: 214–226.

Huang W, Cao L, Liu J H, Lin L S, Dou S Z. 2010. Short-term mercury exposure affecting the development and antioxidant biomarkers of Japanese flounder embryos and larvae. Ecotoxicol. Environ. Saf., 73: 1 875–1 883.

Huang Z Y, Zhang Q, Chen J, Zhuang Z X, Wang X R. 2007. Bioaccumulation of metals and induction of metallothioneins in selected tissues of common carp (Cyprinus carpio L.) co-exposed to cadmium, mercury and lead. Appl. Organometal Chem., 21: 101–107, http://dx.doi.org/10.1002/aoc.1167.

Jezierska B, Witeska M. 2001. Metal toxicity to fish. University of Podlasie, Siedlce, Poland.

Jin X S, Zhao X Y, Meng T X, Cui Y. 2005. Biological resources and their habitats in the Bohai Sea and the Yellow Sea. Science Press, Beijing.

Larose C, Canuel R, Lucotte M, Di Giulio R T. 2008. Toxicological effects of methyl mercury on walleye (Sander vitreus) and perch (Perca flavescens) from lakes of the boreal forest. Comp. Biochem. Physiol. C Toxicol. Pharmacol., 147: 139–149 http://dx.doi.org/10.1016/j.cbpc.2007.09.002.

Liang L N, Shi J B, He B, Jiang G B, Yuan C G. 2003. Investigation of methylmercury and total mercury contamination in mollusk samples collected from coastal sites along the Chinese Bohai Sea. J. Agric. Food Chem., 51: 7 373–7 378, http://dx.doi.org/10.1021/jf034199l.

Liu C, Wang Z Y, He Y, Wu Y S. 2003. Analysis on water quality of the estuary around Bohai Bay. Environ. Pollut. Contr., 25: 222–225. (in Chinese with English abstract)

Livingstone D R. 2001. Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms. Mar. Pollut. Bull., 42: 656–666, http://dx.doi.org/10.1016/S0025-326X (01)00060-1.

Łukaszewicz-Hussain A, Moniuszko-Jakoniuk J. 2004. Liver catalase, glutathione peroxidase and reductase activity, reduced glutathione and hydrogen peroxide levels in acute intoxication with chlorfenvinphos, an organophosphate insecticide. Pol. J. Environ. Stud., 13: 303–309.

Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem., 47: 469–474 http://dx.doi.org/10.1111/j.1432-1033.1974.tb03714.x.

Matta M B, Linse J, Cairncross C, Francendese L, Kocan R M. 2001. Reproductive and transgenerational effects of methylmercury or aroclor 1268 on Fundulus heteroclitus. Environ. Toxicol. Chem., 20: 327–335 http://dx.doi.org/10.1897/1551-5028(2001)020〈0327:RATEOM〉2.0.CO;2.

Middaugh D P, Rose C L. 1974. Retention of two mercurials by striped mullet, Mugil cephalus. Wat. Res., 8: 173–177.

Monteiro D A, de Almeida J A, Rantin F T, Kalinin A L. 2006. Oxidative stress biomarkers in the freshwater characid fish, Brycon cephalus, exposed to organophosphorus insecticide Folisuper 600 (methyl parathion). Comp. Biochem. Physiol. C Toxicol. Pharmacol., 143: 141–149.

Monteiro D A, Rantin F T, Kalinin A L. 2010. Inorganic mercury exposure: toxicological effects, oxidative stress biomarkers and bioaccumulation in the tropical freshwater fish matrinxã, Brycon amazonicus (Spix and Agassiz, 1829). Ecotoxicology, 19: 105–123, http://dx.doi.org/10.1007/s10646-009-0395-1.

Niimi A J, Kissoon G P. 1994. Evaluation of the critical body burden concept based on inorganic and organic mercury toxicity to rainbow trout (Oncorhynchus mykiss). Arch. Environ. Contam. Toxicol., 26: 169–178, http://dx.doi.org/10.1007/BF00224801.

OECD. 1992. Fish, early-life stage toxicity test. OECD Guidelines Testing Chemistry. p.1–18.

Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem., 95: 351–358, http://dx.doi.org/ 10.1016/0003-2697(79)90738-3.

Oliveira M, Pacheco M, Santos M A. 2008. Organ specific antioxidant responses in golden grey mullet (Liza aurata) following a short-term exposure to phenanthrene. Sci. Total Environ., 396: 70–78, http://dx.doi.org/10.1016/j.scitotenv.2008.02.012.

Olson K R, Bergman H L, Fromm P O. 1973. Uptake of methyl mercuric chloride and mercuric chloride by trout: a study of uptake pathways into the whole animal and uptake by erythrocytes in vitro. J. Fish Res. Board Can., 30: 1 293–1 299.

Pandey S, Ahmad I, Parvez S, Bin-Hafeez B, Haque R, Raisuddin S. 2001. Effect of endosulfan on antioxidants of freshwater fish Channa punctatus Bloch. 1. protection against lipid peroxidation in liver by copper pre-exposure. Arch. Environ. Contam. Toxicol., 41: 345–352.

Rana S V S, Singh R, Verma S. 1995. Mercury-induced lipid peroxidation in the liver, kidney, brain and gills of a fresh water fish Channa punctatus. Jpn. J. Ichthyol., 42: 255–259.

Risher J F, Amler S N. 2005. Mercury exposure: evaluation and intervention the inappropriate use of chelating agents in the diagnosis and treatment of putative mercury poisoning. Neurotoxicology, 26: 691–699.

Rothschild R F, Duffy L K. 2005. Mercury concentrations in muscle, brain and bone of Western Alaskan waterfowl. Sci. Total Environ., 349: 277–283, http://dx.doi.org/10.1016/j.scitotenv.2005.05.021.

Rotruck J T, Pope A L, Ganther H E, Swanson A B, Hafeman D G, Hoekstra W G. 1973. Selenium: biochemical role as a component of glutathione peroxidase. Science, 179: 588–590, http://dx.doi.org/10.1126/science.179.4073.588.

SAPRC (Standardization Administration of the People’s Republic of China). 2008. Specification for marine monitoring, mercury analysis in seawater. GB 17378. 4-2007. SAPRC, Beijing. p.2–4.

Thomas P, Wofford H W. 1984. Effects of metal and organic compounds on hepatic glutathione, cysteine and acid soluble thiol levels in mullet (Mugil cephalus L.). Toxicol. Appl. Pharmacol., 76: 172–182, http://dx.doi.org/10.1016/0041-008X (84)90040-1.

Thomaz J M, Martins N D, Monteiro D A, Rantin F T, Kalinin A L. 2009. Cardio-respiratory function and oxidative stress biomarkers in Nile tilapia exposed to the organophosphate insecticide trichlorfon (NEGUVON). Ecotoxicol. Environ. Saf., 72: 1 413–1 424, http://dx.doi.org/10.1016/j.ecoenv.2008.11.003.

Üner N, Oruç E, Sevgiler Y. 2005. Oxidative stress-related and ATPase effects of etoxazole in different tissues of Oreochromis niloticus. Environ. Toxicol. Pharmacol., 20: 99–106, http://dx.doi.org/10.1016/j.etap.2004.11.006.

van der Oost R, Beyer J, Vermeulen N P E. 2003. Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environ. Toxicol. Pharmacol., 13: 57–149, http://dx.doi.org/10.1016/S1382-6689(02)00126-6.

Verlecar X N, Jena K B, Chainy G B N. 2007. Biochemical markers of oxidative stress in Perna viridis exposed to mercury and temperature. Chem. Biol. Interact., 167: 219–226, http://dx.doi.org/10.1016/j.cbi.2007.01.018.

Verlecar X N, Jena K B, Chainy G B N. 2008. Modulation of antioxidant defences in digestive gland of Perna viridis (L.), on mercury exposures. Chemosphere, 71: 1 977–1 985, http://dx.doi.org/10.1016/j.chemosphere.2007.12.014.

Vieira L R, Gravato C, Soares A M V M, Morgado F, Guilhermino L. 2009. Acute effects of copper and mercury on the estuarine fish Pomatoschistus microps: Linking biomarkers to behaviour. Chemosphere, 76: 1 416–1 427, http://dx.doi.org/10.1016/j.chemosphere.2009.06.005.

Wang Q, Liu B Z, Yang H S, Wang X Y, Lin Z H. 2009. Toxicity of lead, cadmium and mercury on embryogenesis, survival, growth and metamorphosis of Meretrix meretrix larvae. Ecotoxicology, 18: 829–837, http://dx.doi.org/10.1007/s10646-009-0326-1.

Weis J S, Khan A A. 1990. Effects of mercury on the feeding behavior of the mummichog, Fundulus heterociitus, from a polluted habitat. Mar. Environ. Res., 30: 243–249, http://dx.doi.org/10.1016/0141-1136 (90)90001-5.

Winston G W, Di Giulio R T. 1991. Prooxidant and antioxidant mechanisms in aquatic organisms. Aquat. Toxicol., 19: 137–161, http://dx.doi.org/10.1016/0166-445X (91)90033-6.

Yang J, Kunito T, Tanabe S, Amano M, Miyazaki N. 2002. Trace elements in skin of Dall’s porpoises (Phocoenoides dalli) from the northern waters of Japan: an evaluation for utilization as non-lethal tracers. Mar. Poll. Bull., 45: 230–236, http://dx.doi.org/10.1016/S0025-326X(01)00328-9.

Yediler A, Jacobs J. 1995. Synergistic effects of temperature; oxygen and water flow on the accumulation and tissue distribution of mercury in carp (Cyprinus carpio L.). Chemosphere, 31: 4 437–4 453, http://dx.doi.org/10.1016/0045-6535(95)00324-2.

Zhang L, Wong M H. 2007. Environmental mercury contamination in China: sources and impacts. Environ. Int., 33: 108–121, http://dx.doi.org/10.1016/j.envint.2006.06.022.

Zhang X L. 2001. Investigation of pollution of Pb, Cd, Hg and As in the sea water and sediments of the Bohai Sea. Heilongjiang Environ. J., 25: 87–90. (in Chinese with English abstract)

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Investigation of the decline of Ulva prolifera in the Subei Shoal and Qingdao based on physiological changes

Treating ballast water with hydroxyl radical on introduced organisms

Antioxidative capacity and enzyme activity in Haematococcus pluvialis cells exposed to superoxide free radicals

Species- and tissue-specific mercury bioaccumulation in five fish species from Laizhou Bay in the Bohai Sea of China

V形前缘对激波入射边界层流动影响的数值模拟与分析