Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish

Dongdong Wang; Fuhua Li; Shihao Li; Rong Wen; Jianhai Xiang

doi:10.1007/s00343-012-1195-7

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Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish

OriginalPaper | Updated：2023-05-16

- Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish
- Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish
- 海洋湖沼学报（英文） 2012年30卷第4期页码：611-619
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (Nos. 31072203, 30230280), the Special Fund for Agro-Scientific Research in the Public Interest (No. 201103034), and the National Basic Research Program of China (973 Program) (No. 2012CB114403)
- DOI：10.1007/s00343-012-1195-7
  中图分类号：
- 网络首发：2012-07-02，
  
  纸质出版：2012
- Accepted：
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Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish[J]. 海洋湖沼学报（英文）, 2012,30(4):611-619.

Wang, D., Li, F., Li, S. et al. Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish., Chin. J. Ocean. Limnol. 30, 611–619 (2012). https://doi.org/10.1007/s00343-012-1195-7
Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish[J]. 海洋湖沼学报（英文）, 2012,30(4):611-619. DOI： 10.1007/s00343-012-1195-7.

Wang, D., Li, F., Li, S. et al. Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish., Chin. J. Ocean. Limnol. 30, 611–619 (2012). https://doi.org/10.1007/s00343-012-1195-7 DOI：

摘要

Abstract

Antimicrobial peptides (AMPs)

as key immune effectors

play important roles in the innate immune system of invertebrates. Different types of AMPs

including Penaeidin

Crustin

ALF (antilipopolysaccharide factor) have been identified in different penaeid shrimp; however

systematic analyses on the function of different AMPs in shrimp responsive to different types of bacteria are very limited. In this study

we analyzed the expression profiles of AMPs in the Chinese shrimps

Fenneropenaeus chinensis

simultaneously by real-time RT-PCR (reverse transcription-polymerase chain reaction) when shrimp were challenged with Micrococcus lysodeikticus (Gram-positive

G+) or Vibrio anguillarium (Gram-negative

G−). Different AMPs showed different expression profiles when shrimp were injected with one type of bacterium

and one AMP also showed different expression profiles when shrimp were challenged with different bacteria. Furthermore

the expression of these AMPs showed temporal expression profiles

suggesting that different AMPs function coordinately in bacteria-infected shrimp. An RNA interference approach was used to study the function of the Relish transcription factor in regulating the transcription of different AMPs. The current study showed that Relish could regulate the transcription of different AMPs in shrimp. Differential expression profiles of AMPs in shrimp injected with different types of bacteria indicated that a complicated antimicrobial response network existed in shrimp. These data contribute to our understanding of immunity in shrimp and may provide a strategy for the control of disease in shrimp.

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references

Amparyup P, Kondo H, Hirono I, Aoki T, Tassanakajon A. 2008. Molecular cloning, genomic organization and recombinant expression of a crustin-like antimicrobial peptide from black tiger shrimp Penaeus monodon. Mol. Immunol., 45(4): 1 085–1 093.

Andrda J, Lamata M, de Tejada G M, Bartels R, Koch M H J, Brandenburg K. 2004. Cyclic antimicrobial peptides based on Limulus anti-lipopolysaccharide factor for neutralization of lipopolysaccharide. Biochem. Pharmacol., 68(7): 1 297–1 307.

Antony S P, Singh I S B, Philip R. 2010. Molecular characterization of a crustin-like, putative antimicrobial peptide, Fi-crustin, from the Indian white shrimp, Fenneropenaeus indicus. Fish Shellfish Immunol., 28(1): 216–220.

Bachere E, Gueguen Y, Gonzalez M, de Lorgeril J, Garnier J, Romestand B. 2004. Insights into the anti-microbial defense of marine invertebrates: the penaeid shrimps and the oyster Crassostrea gigas. Immunol. Rev., 198(1): 149–168.

Bartlett T C, Cuthbertson B J, Shepard E F, Chapman R W, Gross P S, Warr G W. 2002. Crustins, Homologues of an 11.5-kDa Antibacterial Peptide, from Two Species of Penaeid Shrimp, Litopenaeus vannamei and Litopenaeus setiferus. Mar. Biotechnol., 4(3): 278–293.

Belvin M P, Anderson K V. 1996. A conserved signaling pathway: The Drosophila Toll-Dorsal pathway. Annu. Rev. Cell Dev. Biol., 12: 393–416.

Brockton V, Smith V J. 2008. Crustin expression following bacterial injection and temperature change in the shore crab, Carcinus maenas. Dev. Comp. Immunol., 32(9): 1 027–1 033.

Bulet P, Destoumieux D, Strub J M, Van Dorsselaer A, Bachere E. 1999. Recombinant expression and range of activity of penaeidins, antimicrobial peptides from penaeid shrimp. Eur. J. Biochem., 266(2): 335–346.

Cuthbertson B J, Shepard E F, Chapman R W, Gross P S. 2002. Diversity of the penaeidin antimicrobial peptides in two shrimp species. Immunogenetics, 54(6): 442–445.

De Gregorio E, Spellman P T, Tzou P, Rubin G M, Lemaitre B. 2002. The Toll and Imd pathways are the major regulators of the immune response in Drosophila. Embo Journal, 21(11): 2 568–2 579.

Destoumieux D, Bulet P, Loew D, VanDorsselaer A, Rodriguez J, Bachere E. 1997. Penaeidins, a new family of antimicrobial peptides isolated from the shrimp Penaeus vannamei (decapoda). J. Biol. Chem., 272(45): 28 398–28 406.

Gross P S, Bartlett T C, Browdy C L, Chapman R W, Warr G W. 2001. Immune gene discovery by expressed sequence tag analysis of hemocytes and hepatopancreas in the Pacific White Shrimp, Litopenaeus vannamei, and the Atlantic White Shrimp, L-setiferus. Dev. Comp. Immunol., 25(7): 565–577.

Hedengren M, Asling B, Dushay M S, Ando I, Ekengren S, Wihlborg M, Hultmark D. 1999. Relish, a central factor in the control of humoral but not cellular immunity in Drosophila. Mol. Cell, 4(5): 827–837.

Hoffmann J A, Reichhart J M. 2002. Drosophila innate immunity: an evolutionary perspective. Nat. Immunol., 3(2): 121–126.

Huang X D, Yin Z X, Liao J X, Wang P H, Yang L S, Ai H S, Gu Z H, Jia X T, Weng S P, Yu X Q, He J G. 2009. Identification and functional study of a shrimp Relish homologue. Fish Shellfish Immunol., 27(2): 230–238.

Huang X D, Yin Z X, Jia X T, Liang J P, Ai H S, Yang L S, Liu X, Wang P H, Li S D, Weng S P, Yu X Q, He J G. 2010. Identification and functional study of a shrimp Dorsal homologue. Dev. Comp. Immunol., 34(2): 107–113.

Kang C J, Wang J X, Zhao X F, Yang X M, Shao H L, Xiang J H. 2004. Molecular cloning and expression analysis of Ch-penaeidin, an antimicrobial peptide from Chinese shrimp, Fenneropenaeus chinensis. Fish Shellfish Immunol., 16(4): 513–525.

Kang C J, Xue J F, Liu N, Zhao X F, Wang J X. 2007. Characterization and expression of a new subfamily member of penaeidin antimicrobial peptides (penaeidin 5) from Fenneropenaeus chinensis. Mol. Immunol., 44(7): 1 535–1 543.

Khush R S, Leulier F, Lemaitre B. 2001. Drosophila immunity: two paths to NF-kappa B. Trends Immunol., 22(5): 260–264.

Lemaitre B, Nicolas E, Michaut L, Reichhart J M, Hoffmann J A. 1996. The dorsoventral regulatory gene cassette spatzle/Toll/cactus controls the potent antifungal response in Drosophila adults. Cell, 86(6): 973–983.

Lemaitre B, Reichhart J M, Hoffmann J A. 1997. Drosophila host defense: differential induction of antimicrobial peptide genes after infection by various classes of microorganisms. Proc. Natl. Acad. Sci. U. S. A., 94(26): 14 614–14 619.

Lemaitre B, Hoffmann J. 2007. The host defense of Drosophila melanogaster. Annu. Rev. Immunol., 25: 697–743.

Li F H, Yan H, Wang D D, Priya T A J, Li S H, Wang B, Zhang J Q, Xiang J H. 2009. Identification of a novel relish homolog in Chinese shrimp Fenneropenaeus chinensis and its function in regulating the transcription of antimicrobial peptides. Dev. Comp. Immunol., 33(10): 1 093–1 101.

Li F H, Wang D D, Li S H, Yan H, Zhang J K, Wang B, Zhang J Q, Xiang J H. 2010. A Dorsal homolog (FcDorsal) in the Chinese shrimp Fenneropenaeus chinensis is responsive to both bacteria and WSSV challenge. Dev. Comp. Immunol., 34(8): 874–883.

Liu F S, Liu Y C, Li F H, Dong B, Xiang J H. 2005. Molecular cloning and expression profile of putative antilipopolysaccharide factor in Chinese shrimp (Fenneropenaeus chinensis). Mar. Biotechnol., 7(6): 600–608.

Liu F S, Li F H, Xiang J H, Dong B, Liu Y C, Zhang X J, Zhang L S. 2008. Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis. Acta Oceanologica Sinica, 27(2): 81–92.

Munoz M, Vandenbulcke F, Saulnier D, Bachere E. 2002. Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimp. Eur. J. Biochem., 269(11): 2 678–2 689.

Relf J M, Chisholm J R S, Kemp G D, Smith V J. 1999. Purification and characterization of a cysteine-rich 11.5-kDa antibacterial protein from the granular haemocytes of the shore crab, Carcinus maenas. Eur. J. Biochem., 264(2): 350–357.

Rutschmann S, Jung A C, Hetru C, Reichhart J M, Hoffmann J A, Ferrandon D. 2000. The Rel protein DIF mediates the antifungal but not the antibacterial host defense in Drosophila. Immunity, 12(5): 569–580.

Soderhall K, Liu H P, Jiravanichpaisal P, Soderhall I, Cerenius L. 2006. Antilipopolysaccharide factor interferes with white spot syndrome virus replication in vitro and in vivo in the crayfish Pacifastacus leniusculus. J. Virol., 80(21): 10 365–10 371.

Somboonwiwat K, Marcos M, Tassanakajon A, Klinbunga S, Aumelas A, Romestand B, Gueguen Y, Boze H, Moulin G, Bachere E. 2005. Recombinant expression and antimicrobial activity of anti-lipopolysaccharide factor (ALF) from the black tiger shrimp Penaeus monodon. Dev. Comp. Immunol., 29(10): 841–851.

Supungul P, Klinbunga S, Pichyangkura R, Jitrapakdee S, Hirono I, Aoki T, Tassanakajon A. 2002. Identification of immune-related genes in hemocytes of black tiger shrimp (Penaeus monodon). Mar. Biotechnol., 4(5): 487–494.

Supungul P, Klinbunga S, Pichyangkura R, Hirono I, Aoki T, Tassanakajon A. 2004. Antimicrobial peptides discovered in the black tiger shrimp Penaeus monodon using the EST approach. Dis. Aquat. Org., 61(1–2): 123–135.

Supungul P, Tang S, Maneeruttanarungroj C, Rimphanitchayakit V, Hirono I, Aoki T, Tassanakajon A. 2008. Cloning, expression and antimicrobial activity of crustinPm1, a major isoform of crustin, from the black tiger shrimp Penaeus monodon. Dev. Comp. Immunol., 32(1): 61–70.

Tanaka S, Nakamura T, Morita T, Iwanaga S. 1982. Limulus anti-lps factor—an anticoagulant which inhibits the endotoxin-mediated activation of limulus coagulation system. Biochem. Biophys. Res. Commun., 105(2): 717–723.

Vargas-Albores F, Yepiz-Plascencia G, Jiménez-Vega F, Ávila-Villa A. 2004. Structural and functional differences of Litopenaeus vannamei crustins. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 138(4): 415–422.

Wang J X, Li L, Zhao X F, Kang C H, Liu N, Xiang J H, Li F H, Sueda S, Kondo H. 2005. High level expression, purification, and characterization of the shrimp antimicrobial peptide, Ch-penaeidin, in Pichia pastoris. Protein Expr. Purif., 39(2): 144–151.

Zhang J Q, Li F H, Wang Z Z, Xiang J H. 2007. Cloning and recombinant expression of a crustin-like gene from Chinese shrimp, Fenneropenaeus chinensis. J. Biotechnol., 127(4): 605–614.

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