Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

Linbao Zhang; Huifeng Wu; Xiaoli Liu; Leilei Chen; Qing Wang; Jianmin Zhao; Liping You

doi:10.1007/s00343-012-1173-0

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Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure

OriginalPaper | Updated：2023-05-16

- Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure
- Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure
- 海洋湖沼学报（英文） 2012年30卷第3期页码：413-423
- Affiliations：
  
  1. Key Laboratory of Coastal Zone Environmental Processes Yantai Institute of Coastal Zone Research (YIC) Chinese Academy of Sciences (CAS)
  2. Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes YICCAS
  3. Graduate University of Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the 100 Talents Program of Chinese Academy of Sciences and Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology (No. 201115), and the State Oceanic Administration of China
- DOI：10.1007/s00343-012-1173-0
  中图分类号：
- 网络首发：2011-10-24，
  
  纸质出版：2012
- Accepted：
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Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure[J]. 海洋湖沼学报（英文）, 2012,30(3):413-423.

Zhang, L., Wu, H., Liu, X. et al. Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure., Chin. J. Ocean. Limnol. 30, 413–423 (2012). https://doi.org/10.1007/s00343-012-1173-0
Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure[J]. 海洋湖沼学报（英文）, 2012,30(3):413-423. DOI： 10.1007/s00343-012-1173-0.

Zhang, L., Wu, H., Liu, X. et al. Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure., Chin. J. Ocean. Limnol. 30, 413–423 (2012). https://doi.org/10.1007/s00343-012-1173-0 DOI：

摘要

Abstract

Glutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxification of xenobiotics

and also play important roles in antioxidant defense. We identified two glutathione S-transferase isoforms (VpGSTS

sigma GST; VpGSTO

omega GST) from Venerupis philippinarum by RACE approaches. The open reading frames of VpGSTS and VpGSTO were of 612 bp and 729 bp

encoding 203 and 242 amino acids with an estimated molecular mass of 22.88 and 27.94 kDa

respectively. The expression profiles of VpGSTS and VpGSTO responded to heavy metals and benzo[a]pyrene (B[a]P) exposure were investigated by quantitative real-time RT-PCR. The expression of VpGSTS and VpGSTO were both rapidly up-regulated

however

they showed differential expression patterns to different toxicants. Cd displayed stronger induction of VpGSTS expression with an approximately 12-fold increase than that of VpGSTO with a maximum 6.4-fold rise. Cu exposure resulted in similar expression patterns for both VpGSTS and VpGSTO. For B[a]P exposure

the maximum induction of VpGSTO was approximately two times higher than that of VpGSTS. Altogether

these findings implied the involvement of VpGSTS and VpGSTO in host antioxidant responses

and highlighted their potential as a biomarker to Cd and B[a]P exposure.

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