Molecular identification and culture observation on Acrochaete leptochaete (Chaetophoraceae, Chlorophyta) from China

Yunyan Deng; Xiaorong Tang; Bingxin Huang; Linhong Teng; Lanping Ding

doi:10.1007/s00343-012-1130-y

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Molecular identification and culture observation on Acrochaete leptochaete (Chaetophoraceae, Chlorophyta) from China

OriginalPaper | Updated：2023-05-16

- Molecular identification and culture observation on Acrochaete leptochaete (Chaetophoraceae, Chlorophyta) from China
- Molecular identification and culture observation on Acrochaete leptochaete (Chaetophoraceae, Chlorophyta) from China
- 海洋湖沼学报（英文） 2012年30卷第3期页码：476-484
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
  3. Marine Genetics and Breeding Laboratory Ocean University of China
  4. Shantou University
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (Nos. 31070185, 40876081 and 31093440), the Knowledge Innovation Program of Chinese Academy of Sciences (No. KSCX2-YW-Z-018), the Science and Technology Plan Project of Guangdong Province (No. 2011B031100010), the Team Project of Natural Science Foundation of Guangdong Province (No. S2011030005257), the Talent Introduction Projects of Guangdong Province Universities and Colleges, the Grant of Key Laboratory of Integrated Marine Monitoring and Applied Technologies for Harmful Algal Blooms, S.O.A., MATHAB (No. MATHAB20100301), the Science and Technology Plan Project of Shantou City (No. 2011-162), the Startup Projects (Nos. 09400133 and 09400134), and the Grant for Youth Teachers of Shantou University, P. R. China
- DOI：10.1007/s00343-012-1130-y
  中图分类号：
- 网络首发：2011-09-13，
  
  纸质出版：2012
- Accepted：
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Molecular identification and culture observation on Acrochaete leptochaete (Chaetophoraceae, Chlorophyta) from China[J]. 海洋湖沼学报（英文）, 2012,30(3):476-484.

Deng, Y., Tang, X., Huang, B. et al. Molecular identification and culture observation on Acrochaete leptochaete (Chaetophoraceae, Chlorophyta) from China., Chin. J. Ocean. Limnol. 30, 476–484 (2012). https://doi.org/10.1007/s00343-012-1130-y
Molecular identification and culture observation on Acrochaete leptochaete (Chaetophoraceae, Chlorophyta) from China[J]. 海洋湖沼学报（英文）, 2012,30(3):476-484. DOI： 10.1007/s00343-012-1130-y.

Deng, Y., Tang, X., Huang, B. et al. Molecular identification and culture observation on Acrochaete leptochaete (Chaetophoraceae, Chlorophyta) from China., Chin. J. Ocean. Limnol. 30, 476–484 (2012). https://doi.org/10.1007/s00343-012-1130-y DOI：

摘要

Abstract

Acrochaete leptochaete (Huber) Nielsen (Chaetophoraceae

Chlorophyta) was isolated from the macroalgae Chaetomorpha collected from intertidal pools in Rongcheng

Shandong

China. 18S rDNA combined with ITS regions were used to ascertain the morphological identification of the isolated material. Based on the unialgal culture

asexual reproduction and growth characteristics of A. leptochaete were investigated over wide ranges of temperature and irradiance. Results revealed that asexual reproduction of A. leptochaete could be realized by biflagellate zoospores. The zoospores germinated directly to give self-replicating generations. Zoospore germination was bipolar. A temperature range from 13–21°C and a lower irradiance of 36 μmol/(m2·s) were most favorable for the growth of A. leptochaete. Thallus organization

an important taxonomic criterion for the genus Acrochaete

was affected markedly by temperature and irradiance. Our results extend the knowledge about the species’ general biology and its morphological plasticity. For classification and identification of a simple microphytic algae like A. leptochaete

which are traditionally placed in the class Chaetophoraceae

we propose that molecular tools associated with culture observations are applied.

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Keywords

references

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