Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation

Yulin Cui; Peng Jiang; Jinfeng Wang; Fuchao Li; Yingjie Chen; Guoting Zheng; Song Qin

doi:10.1007/s00343-012-1093-z

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Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation

OriginalPaper | Updated：2023-05-16

- Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation
- Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation
- 海洋湖沼学报（英文） 2012年30卷第3期页码：471-475
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
  3. Key Laboratory of Marine Biotechnology Ningbo University
- Author bio：
- Funds：
  
  Supported by the National Basic Research Program of China (973 Program) (No. 2011CB200902), the National High Technology Research and Development Program of China (863 Program) (Nos. 2009AA10Z106, 2009AA064401), the Promotive Research Fund for Excellent Young and Middle-aged Scientists of Shandong Province (No. 2010BSB02009), the National Natural Science Foundation of China (Nos. 40876082, 30870247), and the Science and Technology Project of Qingdao City (No. 09-1-3-59-jch)
- DOI：10.1007/s00343-012-1093-z
  中图分类号：
- 网络首发：2011-06-30，
  
  纸质出版：2012
- Accepted：
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Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation[J]. 海洋湖沼学报（英文）, 2012,30(3):471-475.

Cui, Y., Jiang, P., Wang, J. et al. Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation., Chin. J. Ocean. Limnol. 30, 471–475 (2012). https://doi.org/10.1007/s00343-012-1093-z
Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation[J]. 海洋湖沼学报（英文）, 2012,30(3):471-475. DOI： 10.1007/s00343-012-1093-z.

Cui, Y., Jiang, P., Wang, J. et al. Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation., Chin. J. Ocean. Limnol. 30, 471–475 (2012). https://doi.org/10.1007/s00343-012-1093-z DOI：

摘要

Abstract

Platymonas (Tetraselmis) subcordiformis is a unicellular marine green alga. It was found to be very sensitive to the herbicide Basta through a sensitivity test indicating it could be employed as a selective agent. The bar gene is a practicable and selectable marker gene. The vector containing the expression cassette of the bar gene was transferred to P. subcordiformis by both particle bombardment and glass-bead agitation and transformants were then selected using Basta. Finally

Southern blotting analysis indicated that the bar gene had been successfully integrated into the nuclear genome of P. subcordiformis using both of the transgenic techniques

with the transformation efficiency of the glass-bead method being slightly higher than that of particle bombardment. This is the first report on stable transformation of P. subcordiformis

and will improve fundamental research and enlarge application of this alga.

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Keywords

references

Cui Y L, Wang J F, Jiang P, Bian S G, Qin S. 2010. Transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) by agitation with glass beads. World J. Microbiol. Biotechnol., 26: 1 653–1 657.

Dawson H N, Burlingame R, Cannons A C. 1997. Stable transformation of Chlorella: rescue of nitrate reductasedeficient mutants with the nitrate reductase gene. Curr. Microbiol., 35: 356–362.

Gan S Y, Qin S, Othman R Y, Yu D, Phang S-M. 2003. Transient expression of lacZ in particle bombarded Gracilaria changii (Gracilariales, Rhodophyta). J. Appl. Phycol., 15: 351–353.

Geng D G, Wang Y Q, Li W B, Sun Y R. 2003. Stable expression of hepatitis B surface antigen gene in Dunaliella salina (Chlorophyta). J. Appl. Phycol., 15: 451–456.

Guan Y F, Zhang W, Deng M C, Jin M F, Yu X J. 2004. Significant enhancement of photobiological H2 evolution by carbonylcyanide m-chlorophenylhydrazone in the marine green alga Platymonas subcordiformis. Biotechnol. Lett., 26: 1 031–1 035.

Jiang P, Qin S, Tseng C K. 2003. Expression of the lacZ reporter gene in sporophytes of the seaweed Laminaria japonica (Phaeophyceae) by gametophyte-targeted transformation. Plant Cell Rep., 21: 1 211–1 216.

Kathiresan S, Chandrashekar A, Ravishankar G A, Sarada R. 2009. Agrobacterium-mediated transformation in the green alga Haematococcus pluvialis (Chlorophyceae, Volvocales). J. Phycol., 45: 642–649.

Kim D H, Kim Y T, Cho J J, Bae J H, Hur S B, Hwang I, Choil T J. 2002. Stable integration and functional expression of flounder growth hormone gene in transformed microalga, Chlorella ellipsoidea. Mar. Biotechnol., 4: 63–73.

Kindle K L. 1990. High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci., 87: 1 228–1 232.

Kindle K L, Richards K L, Stern D. 1991. Engineering the chloroplast genome: techniques and capabilities for chloroplast transformation in Chlamydomonas reinhardtii. Proc. Nati. Acad. Sci., 88: 1 721–1 725.

Liu Y, Yang H B, Yu Y, Li Y M, LV F R. 2007. Studies on the property and structural character of polysaccharide from Platymonas subcordiformis (Will) Hanzen. J. Biol., 24(3): 29–32. (in Chinese with English abstract)

Neuhaus G, Neuhaus-Url G, Groot E J, Schweiger H. 1986. High yield and stable transformation of the unicellular green alga Acetabularia by microinjection of SV40 DNA and pSV2neo. EMBO J., 5: 1 437–1 444.

Qin S, Yu D Z, Jiang P, Teng C Y, Zeng C K. 2003. Stable expression of lacZ reporter gene in seaweed Undaria pinnatifida. High Technol. Lett., 7: 87–89. (in Chinese with English abstract)

Sodeinde O A, Kindle K L. 1993. Homologous recombination in the nuclear genome of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci., 90: 9 199–9 203.

Steinbrenner J, Sandman G. 2006. Transformation of the green alga Haematococcus pluvialis with a phytoene desaturase for accelerated astaxanthin biosynthesis. Appl. Environ. Microbiol., 72: 7 477–7 484.

Tan C, Qin S, Zhang Q, Jiang P, Zhao F. 2005. Establishment of a micro-particle bombardment transformation system for Dunaliella salina. J. Microbiol., 43(4): 361–365.

Teng C, Qin S, Liu J, Yu D, Liang C, Tseng C. 2002. Transient expression of lacZ in bombarded unicellular green alga Haematococcus pluviali s. J. Appl. Phycol., 14: 495–500.

Wang J F, Jiang P, Cui Y, Guan X, Qin S. 2010. Gene transfer into conchospores of Porphyra haitanensis (Bangiales, Rhodophyta). Phycologia, 49: 355–360.

Xie J, Zhang Y, Li Y, Wang Y. 2001. Mixotrophic cultivation of Platymonas subcordiformis. J. Appl. Phycol., 13: 343–347.

Zhang Y, Gao J, Zhang Z, Jiang P, Qin S. 2006. Stable expression of bar gene in the gametophyte of kelp (Laminaria japonica). High Technol. Lett., 16(9): 970–974. (in Chinese with English abstract)

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