Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare

Jin Zhang; Hong Zou; Liangfa Wang; Bei Huang; Nan Li; Guitang Wang; Pin Nie

doi:10.1007/s00343-012-1077-z

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Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare

OriginalPaper | Updated：2023-05-16

- Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare
- Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare
- 海洋湖沼学报（英文） 2012年30卷第2期页码：269-278
- Affiliations：
  
  1. State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Basic Research Program of China (973 Program) (No. 2009CB118703)
- DOI：10.1007/s00343-012-1077-z
  中图分类号：
- 网络首发：2012-03-01，
  
  纸质出版：2012
- Accepted：
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Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare[J]. 海洋湖沼学报（英文）, 2012,30(2):269-278.

Zhang, J., Zou, H., Wang, L. et al. Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare, ., Chin. J. Ocean. Limnol. 30, 269–278 (2012). https://doi.org/10.1007/s00343-012-1077-z
Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare[J]. 海洋湖沼学报（英文）, 2012,30(2):269-278. DOI： 10.1007/s00343-012-1077-z.

Zhang, J., Zou, H., Wang, L. et al. Construction of two selectable markers for integrative/conjugative plasmids in Flavobacterium columnare, ., Chin. J. Ocean. Limnol. 30, 269–278 (2012). https://doi.org/10.1007/s00343-012-1077-z DOI：

摘要

Abstract

Flavobacterium columnare

the etiological agent of columnaris disease

is one of the most important and widespread bacterial pathogens of freshwater fish. In this study

we constructed two artificial selectable markers (chloramphenicol and spectinomycin resistance) for gene transfer in F. columnare. These two new artificial selectable markers

which were created by placing the chloramphenicol or spectinomycin resistance gene under the control of the native acs regulatory region of F. columnare

were functional in both F. columnare and Escherichia coli. The integrative/conjugative plasmids constructed by using these markers were introduced into F. columnare G4 via electroporation or conjugation. The integrated plasmid DNA was confirmed by Southern blotting and PCR analysis. These two markers can be employed in future investigations into gene deletion and the pathogenicity of virulence factors in F. columnare.

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references

Agarwal S, Hunnicutt D W, McBride M J. 1997. Cloning and characterization of the Flavobacterium johnsoniae (Cytophaga johnsonae) gliding motility gene, gldA. Proc. Natl. Acad. Sci. U.S.A., 94: 12 139–12 144.

Altschul S F, Gish W, Miller W, Myers E W, Lipman D J. 1990. Basic local alignment search tool. J. Mol. Biol., 215: 403–410.

Alvarez B, Secades P, McBride M J, Guijarro J A. 2004. Development of genetic techniques for the psychrotrophic fish pathogen Flavobacterium psychrophilum. Appl. Environ. Microbiol., 70: 581–587.

Alvarez B, Alvarez J, Menéndez A, Guijarro J A. 2008. A mutant in one of two exbD loci of a TonB system in Flavobacterium psychrophilum shows attenuated virulence and confers protection against cold water disease. Microbiology, 154: 1 144–1 151

Bader J A, Shoemaker C A, Klesius P H. 2003a. Rapid detection of columnaris disease in channel catfish (Ictalurus punctatus) with a new species-specific 16-S rRNA gene-based primer for Flavobacterium columnare. J. Microbiol. Methods, 52: 209–220.

Bader J A, Nusbaum K E, Shoemaker C A. 2003b. Comparative challenge model of Flavobacterium columnare using abraded and unabraded channel catfish, Ictalurus punctatus (Rafinesque). J. Fish Dis., 26: 461–467.

Bayley D P, Rocha E R, Smith C J. 2000. Analysis of cepA and other Bacteroides fragilis genes reveals a unique promoter structure. FEMS Microbiol. Lett., 193: 149–154.

Berg P. 1956. Acyl adenylates; an enzymatic mechanism of acetate activation. J. Biol. Chem., 222: 991–1 013.

Bernardet J F, Segers P, Vancanneyt M, Berthe F, Kersters K, Vandamme P. 1996. Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. Nov (Basonym, Cytophaga aquatilis Strohl and Tait, 1978). Int. J. Syst. Bacteriol., 46: 128–148.

Black T A, Cai Y, Wolk C P. 1993. Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Mol. Microbiol., 9: 77–84.

Bouhenni R, Gehrke A, Saffarini D. 2005. Identification of genes involved in cytochrome c biogenesis in Shewanella oneidensis, using a modified mariner transposon. Appl. Environ. Microbiol., 71: 4 935–4 937.

Braun T F, Khubbar M K, Saffarini D A, McBride M J. 2005. Flavobacterium johnsoniae gliding motility genes identified by mariner mutagenesis. J. Bacteriol., 187: 6 943–6 952.

Braun T F, McBride M J. 2005. Flavobacterium johnsoniae GldJ is a lipoprotein that is required for gliding motility. J. Bacteriol., 187: 2 628–2 637.

Chen S, Bagdasarian M, Bates A K, Kaufman M G, Walker E D. 2007a. Mutational analysis of ompA promoter in Flavobacterium johnsoniae. J. Bacteriol., 189: 5 108–5 118.

Chen S, Bagdasarian M, Kaufman M G, Walker E D. 2007b. Characterization of strong promoters from an environmental Flavobacterium hibernum strain by using a green fluorescent protein-based reporter system. Appl. Environ. Microbiol., 73: 1 089–1 100.

Cozzone A J. 1998. Regulation of acetate metabolism by protein phosphorylation in enteric bacteria. Ann. Rev. Microbiol., 52: 127–164.

Darwish A M, Ismaiel A A, Newton J C, Tang J. 2004. Identification of Flavobacterium columnare by a speciesspecific polymerase chain reaction and renaming of ATCC43622 strain to Flavobacterium johnsoniae. Mol. Cell Probes, 18: 421–427.

Decostere A, Haesebrouck F, Devriese L A. 1997. Shieh medium supplemented with tobramycin for selective isolation of Flavobacterium columnare (Flexibacter columnaris) from diseased fish. J. Clin. Microb., 35: 322–324.

Decostere A, Haesebrouck F, Devriese L A. 1998. Characterization of four Flavobacterium columnare (Flexibacter columnaris) strains isolated from tropical fish. Vet. Microbiol., 62: 35–45.

Decostere A, Haesebrouck F, Van Driessche E, Charlier G, Ducatelle R. 1999a. Characterization of the adhesion of Flavobacterium columnare (Flexibacter columnaris) to gill tissue. J. Fish Dis., 22: 465–474.

Decostere A, Haesebrouck F, Charlier G, Ducatelle R. 1999b. The association of Flavobacterium columnare strains of high and low virulence with gill tissue of black mollies (Poecilia sphenops). Vet. Microbiol., 67: 287–298.

Figueiredo H C P, Klesius P H, Arias C R, Evans J, Shoemaker C A, Pereira D J Jr, Peixoto M T D. 2005. Isolation and characterization of strains of Flavobacterium columnare from Brazil. J. Fish Dis., 28: 199–204.

Griffin B R. 1991. Characteristics of a chondroitin AC lyase produced by Cytophaga columnaris. Trans. Am. Fish. Soc., 120: 391–395.

Guiney D G, Hasegawa P, Bouic K, Matthews B. 1989. Genetic transfer systems in Bacteroides: cloning and mapping of the transferable tetracycline-resistance locus. Mol. Microbiol., 3: 1 617–1 623.

Hawley D K, McClure W R. 1983. Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res., 11: 2 237–2 255.

Hunnicutt D W, McBride M J. 2000. Cloning and characterization of the Flavobacterium johnsoniae gliding motility genes gldD and gldE. J. Bacteriol., 183: 4 167–4 175.

Kempf M J, McBride M J. 2000. Transposon insertions in the Flavobacterium johnsoniae ftsX gene disrupt gliding motility and cell division. J. Bacteriol., 182: 1 671–1 679.

Koski P, Hiervelä-Koski V, Bernardet J F. 1993. Flexibacter columnaris infection in arctic char (Salvelinus alpinus L.): First isolation in Finland. Bull. Eur. Assoc. Fish Pathol., 13: 66–69.

Liu G Y, Nie P, Zhang J, Li N. 2008. Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Flavobacterium columnare. J. Fish Dis., 31: 269–276.

Lu Q Z, Ni D S, Ge R F. 1975. Studies on the gill diseases of the grass carp (Ctenopharyngodon idellus): I. Isolation of a myxobacterial pathogen. Acta Hydrobiol. Sin., 5: 315–334. (in Chinese with English abstract)

MacLean L L, Perry M B, Crump E M, Kay W W. 2003. Structural characterization of the lipopolysaccharide Opolysaccharide antigen produced by Flavobacterium columnare ATCC 43622. Eur. J. Biochem., 270: 3 440–3 446.

McBride M J, Kempf M J. 1996. Development of techniques for the genetic manipulation of the gliding bacterium Cytophaga johnsonae. J. Bacteriol., 178: 583–590.

McBride M J, Braun T F, Brust J L. 2003. Flavobacterium johnsoniae GldH is a lipoprotein that is required for gliding motility and chitin utilization. J. Bacteriol., 185: 6 648–6 657.

Nematollahi A, Decostere A, Pasmans F, Ducatelle R, Haesebrouc F. 2003a. Adhesion of high and low virulence Flavobacterium psychrophilum strains to isolated gill arches of rainbow trout Oncorhynchus mykiss. Dis. Aquat. Org., 55: 101–107.

Nematollahi A, Decostere A, Pasmans F, Haesebrouc F. 2003b. Flavobacterium psychrophilum infections in salmonid fish. J. Fish Dis., 26: 563–574.

Ringquist S, Shinedling S, Barrick D, Green L, Binkley J, Stormo G D, Gold L. 1992. Translation initiation in Escherichia coli: sequences within the ribosome-binding site. Mol. Microbiol., 6: 1 219–1 229.

Sambook J, Russell D W. 2001. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, Cold Spring Harbor Laboratory Press, NY.

Salyers A A, Shoemaker N, Cooper A, D’Elia J, Shipman J A. 1999. Genetic methods for Bacteroides species. In: Smith M C M, Sockett R E eds. Genetic Methods for Diverse Prokaryotes. Academic Press, London, United Kingdom. p.229–249.

Shieh H S. 1980. Studies on the nutrition of a fish pathogen, Flexibacter columnaris. Microbios. Lett., 13: 129–133.

Shoemaker N B, Getty C, Gardner J F, Salyers A A. 1986. Tn4351 transposes in Bacteroides spp. and mediates the integration of plasmid R751 into the Bacteroides chromosome. J. Bacteriol., 165: 929–936.

Shoemaker N B, Anderson K L, Smithson S L, Wang G R, Salyers A A. 1991. Conjugal transfer of a shuttle vector from the human colonic anaerobe Bacteroides uniformis to the ruminal anaerobe Prevotella (Bacteroides) ruminicola B14. Appl. Environ. Microbiol., 57: 2 114–2 120.

Shotts E B, Starliper C E. 1999. Flavobacterial diseases: columnaris disease, cold-water disease and bacterial gill disease. In: Woo P T K, Bruno D W eds. Fish Disease and Disorders, Vol. 3: Viral, Bacterial and Fungal Infections. CABI Publishing, Oxon. p.559–576.

Simon R, Priefer U, Pühler A. 1983. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Biotechnology, 1: 784–791.

Smith C J, Rogers M B, McKee M L. 1992 Heterologous gene expression in Bacteroides fragilis. Plasmid, 27: 141–154.

Staroscik A M, Hunnicutt D W, Archibald K E, Nelson D R. 2008. Development of methods for the genetic manipulation of Flavobacterium columnare. BMC Microbiol., 8: 115–125.

Stringer-Roth K M, Yunghans W, Caslake L F. 2002. Differences in chondroitin AC lyase activity of Flavobacterium columnare isolates. J. Fish Dis., 25: 687–691.

Suomalainen L R, Tiirola M, Valtonen E T. 2006. Chondroitin AC lyase activity is related to virulence of fish pathogenic Flavobacterium columnare. J. Fish Dis., 29: 757–763.

Teska J D. 1993. Assay to evaluate the reaction kinetics of chondroitin AC lyase produced by Cytophaga columnaris. J. Aquat. Anim. Health, 5: 259–264.

Wagner B A, Wise D J, Khoo L H, Terhune J S. 2002. The epidemiology of bacterial diseases in food-size channel catfish. J. Aquat. Anim. Health, 14: 263–272.

Welker T L, Shoemaker C A, Arias C R, Klesius P H. 2005. Transmission and detection of Flavobacterium columnare in channel catfish Ictalurus punctatus. Dis. Aquat. Organ., 63: 129–138.

Xie H X, Nie P, Chang M X, Liu Y, Yao W J. 2005. Gene cloning and functional analysis of glycosaminoglycandegrading enzyme chondroitin AC lyase from Flavobacterium columnare G4. Arch. Microbiol., 18: 49–55.

Xie H X, Nie P, Sun B J. 2004. Characterization of two membrane-associated protease genes obtained from screening out-membrane protein genes of Flavobacterium columnare G4. J. Fish Dis., 27: 719–729.

Zhang J, Zou H, Yao W J, Nie P. 2011. Acetyl-coenzyme A synthetase gene and its upstream promoter in the bacterial pathogen Flavobacterium columnare. J. Fishery Sciences of China, 18: 1 100–1 107. (in Chinese with English abstract)

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