Toxic effects of Karenia mikimotoi extracts on mammalian cells

Yang Chen; Tian Yan; Rencheng Yu; Mingjiang Zhou

doi:10.1007/s00343-011-0514-8

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Toxic effects of Karenia mikimotoi extracts on mammalian cells

OriginalPaper | Updated：2023-05-16

- Toxic effects of Karenia mikimotoi extracts on mammalian cells
- Toxic effects of Karenia mikimotoi extracts on mammalian cells
- 海洋湖沼学报（英文） 2011年29卷第4期页码：860-868
- Affiliations：
  
  Key Laboratory of Marine Ecology and Environmental Science Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Basic Research Program of China (973 Program) (No. 2010CB428700), the National Natural Science Foundation of China (Nos. U0733006, 40821004), and the Knowledge Innovation Program of the Chinese Academy of Sciences (No. KZCX2-YWQ07-02)
- DOI：10.1007/s00343-011-0514-8
  中图分类号：
- 网络首发：2011-07-10，
  
  纸质出版：2011
- Accepted：
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Toxic effects of Karenia mikimotoi extracts on mammalian cells[J]. 海洋湖沼学报（英文）, 2011,29(4):860-868.

Chen, Y., Yan, T., Yu, R. et al. Toxic effects of Karenia mikimotoi extracts on mammalian cells., Chin. J. Ocean. Limnol. 29, 860–868 (2011). https://doi.org/10.1007/s00343-011-0514-8
Toxic effects of Karenia mikimotoi extracts on mammalian cells[J]. 海洋湖沼学报（英文）, 2011,29(4):860-868. DOI： 10.1007/s00343-011-0514-8.

Chen, Y., Yan, T., Yu, R. et al. Toxic effects of Karenia mikimotoi extracts on mammalian cells., Chin. J. Ocean. Limnol. 29, 860–868 (2011). https://doi.org/10.1007/s00343-011-0514-8 DOI：

摘要

Abstract

Karenia is one of the most harmful and representative red tide genus in a temperate zone. Blooms caused by this genus have resulted in massive fish death in the South China Sea and the East China Sea. However

the potential effects of this dinoflagellate on human health through the transfer of toxins via marine food webs

and the mechanisms of toxicity

are still unknown. Therefore

we examined the toxic effects of a strain of K. mikimotoi (isolated from the South China Sea) on the proliferation and morphology of four mammalian cell lines (two normal cell lines and two cancer cell lines). In addition

we carried out a preliminary investigation on the mechanism of toxicity of the alga. The results show that the polar lipid-soluble component of K. mikimotoi significantly inhibited proliferation of the four cell lines

and resulted in the cells becoming spherical

swollen and damaged. The result of Annexin V and PI double-staining confirmed that cell membranes were disrupted. The malonaldehyde (MDA) contents in the medium of the four cell lines treated with the polar-lipid extracts all increased significantly

which indicates that the polar-lipid toxins produced by K. mikimotoi could adversely affect mammalian cells by inducing lipid peroxidation. We conclude that K. mikimotoi is a potential threat to human health

and the comprehensive effect of this dinoflagellate and its mechanisms should be investigated further.

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references

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