Effects of ambient DIN:DIP ratio on the nitrogen uptake of harmful dinoflagellate Prorocentrum minimum and Prorocentrum donghaiense in turbidistat

Ji Li; Patricia M. Glibert; Jeffrey A. Alexander

doi:10.1007/s00343-011-0504-x

Your Location：

Home >

Browse articles >

Effects of ambient DIN:DIP ratio on the nitrogen uptake of harmful dinoflagellate Prorocentrum minimum and Prorocentrum donghaiense in turbidistat

OriginalPaper | Updated：2023-05-16

- Effects of ambient DIN:DIP ratio on the nitrogen uptake of harmful dinoflagellate Prorocentrum minimum and Prorocentrum donghaiense in turbidistat
- Effects of ambient DIN:DIP ratio on the nitrogen uptake of harmful dinoflagellate Prorocentrum minimum and Prorocentrum donghaiense in turbidistat
- 海洋湖沼学报（英文） 2011年29卷第4期页码：746-761
- Affiliations：
  
  Horn Point Laboratory University of Maryland Center for Environmental Science
- Author bio：
- Funds：
- DOI：10.1007/s00343-011-0504-x
  中图分类号：
- 网络首发：2011-07-10，
  
  纸质出版：2011
- Accepted：
Scan QR Code
Effects of ambient DIN:DIP ratio on the nitrogen uptake of harmful dinoflagellate Prorocentrum minimum and Prorocentrum donghaiense in turbidistat[J]. 海洋湖沼学报（英文）, 2011,29(4):746-761.

Li, J., Glibert, P.M. & Alexander, J.A. Effects of ambient DIN:DIP ratio on the nitrogen uptake of harmful dinoflagellate Prorocentrum minimum and Prorocentrum donghaiense in turbidistat., Chin. J. Ocean. Limnol. 29, 746–761 (2011). https://doi.org/10.1007/s00343-011-0504-x
Effects of ambient DIN:DIP ratio on the nitrogen uptake of harmful dinoflagellate Prorocentrum minimum and Prorocentrum donghaiense in turbidistat[J]. 海洋湖沼学报（英文）, 2011,29(4):746-761. DOI： 10.1007/s00343-011-0504-x.

Li, J., Glibert, P.M. & Alexander, J.A. Effects of ambient DIN:DIP ratio on the nitrogen uptake of harmful dinoflagellate Prorocentrum minimum and Prorocentrum donghaiense in turbidistat., Chin. J. Ocean. Limnol. 29, 746–761 (2011). https://doi.org/10.1007/s00343-011-0504-x DOI：

摘要

Abstract

The effects of varying nitrogen (N): phosphorus (P) ratios on the growth and N-uptake and assimilation of the harmful dinoflagellates Prorocentrum minimum and Prorocentrum donghaiense were examined in turbidistat culture experiments. Algal cultures were supplied with media containing PO 4 3− in various concentrations to obtain a wide range of N:P ratios. Experiments to determine rates of N uptake and assimilation of different N sources (NO 3 −

NH 4 +

urea and glycine by P. minimum and NO 3 −

NH 4 + by P. donghaiense) were conducted using 15N tracer techniques at each N:P ratio. The growth rates suggested nutrient limitation at both high and low N:P ratios relative to the Redfield ratio. On a diel basis

the growth of both species was regulated by the light-dark cycle

which may be a result of regulation of both lightdependent growth and light-independent nutrient uptake. Maximum growth rates of both species always occurred at the beginning of light phase. In P-rich medium (low N:P ratio)

both species had higher N assimilation rates

suggesting N limitation. Low assimilation coefficients at high N:P ratios suggested P limitation of N uptake and assimilation. NO 3 − and NH 4 + contributed more than 90% of the total N uptake of P. minimum. Reduced N sources were more quickly assimilated than NO 3 − . Highest average daily growth rates were recorded near an N:P ratio of 12 for both species. The N uptake rates of cultures at N:P ratios near Redfield ratio were more balanced with growth rates. The linkage between growth rates and N uptake/assimilation rates were conceptually described by the variation of cell N quota. The N:P ratios affect the N uptake and growth of Prorocentrum spp.

and may regulate their bloom progression in eutrophic ecosystems.

关键词

Keywords

references

Anderson D M, Burkholder J M, Cochlan W P, Glibert P M, Gobler C J, Heil C A, Kudela R M, Parsons M L, Rensel J E J, Townsend D W, Trainer V L, Vargo G A. 2008. Harmful algal blooms and eutrophication: Examining linkages from selected coastal regions of the united states. Harmful Algae, 8(SI): 39–53.

Anderson D M, Glibert P M, Burkholder J M. 2002. Harmful algal blooms and eutrophication: Nutrient sources, composition, and consequences. Estuaries, 25(4B): 704–726.

Bennett W N, Boraas M E. 1989. Comparison of population-dynamics between slow-growing and fast-growing strains of the rotifer Brachionus calyciflorus pallas in continuous culture. Oecologia., 81(4): 494–500.

Bulgakov N G, Levich A P. 1999. The nitrogen: Phosphorus ratio as a factor regulating phytoplankton community structure. Arch. Hydrobiol., 146(1): 3–22.

Burns C L, Pennock J R, Lores E M, R M G. 2000. The effect of nitrogen source on the growth and toxicity of three potentially harmful dinoflagellates. J. Phycol., 36(3). (Abstract only)

Chisholm S W, Nobbs P A, Stross R G. 1975. Simulation of algal growth and competition in a phosphate-limited cyclostat. Abstracts of Papers of the American Chemical Society, 42–42.

Degens E T, Mopper K. 1976. Factors controlling distribution and early diagenesis of organic marine sediments. In: Riley J P, Chester R eds. Chemical oceanography, Vol.6. Academic London, New York, San Francisco. p.60–114.

Doemel W N, Brooks A E. 1975. Detergent phosphorus and algal growth. Water Res., 9(8): 713–719.

Droop M R. 1968. Vitamin B12 and marine ecology 4. Kinetics of uptake growth and inhibition in Monochrysis lutheri. J. Mar. Biol. Assoc. U.K., 48: 689–733.

Droop M R. 1973. Some thoughts on nutrient limitation in algae. J. Phycol., 9(3): 264–272.

Eppley R W, Renger E H. 1974. Nitrogen assimilation of an oceanic diatom in nitrogen-limited continuous culture. J. Phycol., 10(1): 15–23.

Falkowski P G, Dubinsky Z, Wyman K. 1985. Growthirradiance relationships in phytoplankton. Limnol. Oceanogr., 30(2): 311–321.

Fan C, Glibert P M, Alexander J, Lomas M W. 2003a. Characterization of urease activity in three marine phytoplankton species, Aureococcus anophagefferens, Prorocentrum minimum, and Thalassiosira weissflogii. Mar. Biol., 142(5): 949–958.

Fan C L, Glibert P M. 2005. Effects of light on nitrogen and carbon uptake during a Prorocentrum minimum bloom. Harmful Algae, 4(3): 629–641.

Fan C L, Glibert P M, Burkholder J M. 2003b. Characterization of the affinity for nitrogen, uptake kinetics, and environmental relationships for Prorocentrum minimum in natural blooms and laboratory cultures. Harmful Algae, 2(4): 283–299.

Fenaux R, Malara G, Claustre H. 1985. A turbidostat driven and controlled by microcomputer. Aquaculture, 48(1): 91–95.

Fisher T R, Peele E R, Ammerman J W, Harding L W. 1992. Nutrient limitation of phytoplankton in Chesapeake Bay. Mar. Ecol. Prog. Ser., 82(1): 51–63.

Frisch H L, Gotham I J. 1977. Periodic algal cyclostat populations. J. Theor. Biol., 66(4): 665–678.

Glibert P M, Anderson D M, Gentien P, Graneli E, Sellner K G. 2005. The global, complex phenomena of harmful algal blooms. Oceanography, 18(2): 136–147.

Glibert P M, Burkholder J M. 2006. The complex relationships between increasing fertilization of the earth, coastal eutrophication and proliferation of harmful algal blooms. In: Graneli E, Turner J eds. Ecology of Harmful Algae. Springer. p.341–354.

Glibert P M, Burkholder J M, Graneli E, Anderson D M. 2008a. Advances and insights in the complex relationships between eutrophication and HABs: Preface to the special issue. Harmful Algae, 8(SI): 1–2.

Glibert P M, Capone D G. 1993. Mineralization and assimilation in aquatic, sediment, and wetland systems. In: Knowles R, Blackburn T H eds. Nitrogen Isotope Techniques. p.243–272. Academic Press.

Glibert P M, Harrison J, Heil C, Seitzinger S. 2006. Escalating worldwide use of urea—a global change contributing to coastal eutrophication. Biogeochemistry, 77(3): 441–463.

Glibert P M, Mayorga E, Seitzinger S. 2008b. Prorocentrum minimum tracks anthropogenic nitrogen and phosphorus inputs on a global basis: Application of spatially explicit nutrient export models. Harmful Algae, 8(SI): 33–38.

Glibert P M, Terlizzi D E. 1999. Cooccurrence of elevated urea levels and dinoflagellate blooms in temperate estuarine aquaculture ponds. Appl. Environ. Microbiol., 65(12): 5 594–5 596.

Goldman J C, Glibert P M. 1982. Comparative rapid ammonium uptake by 4 species of marine-phytoplankton. Limnol. Oceanogr., 27(5): 814–827.

Gotham I J, Frisch H L. 1981. A simple-model for cell-volume and developmental compartments in nutrient limited cyclostat cultures of algae. J. Theor. Biol., 92(4): 435–467.

Gotham I J, Rhee G Y. 1981. Comparative kinetic-studies of phosphate-limited growth and phosphate-uptake in phytoplankton in continuous culture. J. Phycol., 17(3): 257–265.

Gotham I J, Rhee G Y. 1982. Effects of nitrate and phosphate limitation on cyclostat growth of 2 fresh-water diatoms. J. Gen. Microbiol., 128(1): 199–205.

Guillard R R L, Ryther J H. 1962. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea Cleve. Can. J. Microbiol., 8(2): 229–239.

Hallegraeff G M. 1993. A review of harmful algal blooms and their apparent global increase. Phycologia, 32(2): 79–99.

Harding L W, Coats D W. 1988. Photosynthetic physiology of Prorocentrum mariae-lebouriae (dinophyceae) during its subpycnocline transport in Chesapeake Bay. J. Phycol., 24(1): 77–89.

Harding L W, Meeson B W, Tyler M A. 1983. Photoadaptation and diel periodicity of photosynthesis in the dinoflagellate Prorocentrum mariae-lebouriae. Mar. Ecol. Prog. Ser., 13(1): 73–85.

Hartig J H, Horvath F J. 1982. A preliminary assessment of Michigan phosphorus detergent ban. J. Water Pollut. Control Fed., 54(2): 193–197.

Heil C A, Glibert P M, Fan C L. 2005. Prorocentrum minimum (pavillard) schiller—a review of a harmful algal bloom species of growing worldwide importance. Harmful Algae, 4(3): 449–470.

Heil C A, Revilla M, Glibert P M, Murasko S. 2007. Nutrient quality drives differential phytoplankton community composition on the southwest Florida shelf. Limnol. Oceanogr., 52(3): 1 067–1 078.

Heisler J, Glibert P M, Burkholder J M, Anderson D M, Cochlan W, Dennison W C, Dortch Q, Gobler C J, Heil C A, Humphries E, Lewitus A, Magnien R, Marshall H G, Sellner K, Stockwell D A, Stoecker D K, Suddleson M. 2008. Eutrophication and harmful algal blooms: A scientific consensus. Harmful Algae, 8(SI): 3–13.

Hill S H, Abbott M R, Denman K L. 1985. A computer-controlled turbidostat for the culture of planktonic algae. Can. J. Fish. Aquat. Sci., 42(4): 744–753.

Hirsbrunner M. 1981. A Chemostatanlage zur kontinuierlichen Kultur von Algen. Schweiz. Z. Hydrol., 43(2): 370–376.

Hodgkiss I J, Ho K C. 1997. Are changes in N:P ratios in coastal waters the key to increased red tide blooms? Hydrobiologia, 352: 141–147.

Jeong H J, Du Y Y, Park J Y, Song J Y, Kim S T, Lee S H, Kim K Y, Yih W H. 2005a. Feeding by phototrophic red-tide dinoflagellates: Five species newly revealed and six species previously known to be mixotrophic. Aquat. Microb. Ecol., 40(2): 133–150.

Jeong H J, Park J Y, Nho J H, Park M O, Ha J H, Seong K A, Jeng C, Seong C N, Lee K Y, Yih W H. 2005b. Feeding by red-tide dinoflagellates on the cyanobacterium Synechococcus. Aquat. Microb. Ecol., 41(2): 131–143.

Kalyuzhin V A. 1998. The growth of a turbidostat yeast culture in the presence of high concentrations of various compounds in a steady-state regime and under osmotic shock. Microbiology, 67(5): 499–503.

Lagus A, Suomela J, Weithoff G, Heikkila K, Helminen H, Sipura J. 2004. Species-specific differences in phytoplankton responses to N and P enrichments and the N:P ratio in the Archipelago sea, Northern Baltic Sea. J. Plankton Res., 26(7): 779–798.

Lane L, Rhoades S, Thomas C, Van Heukelem L. 2000. Analytical Services Laboratory Standard Operating Procedures. University of Maryland Center for Environmental Science, Cambridge, Maryland.

Li A S, Stoecker D K, Coats D W, Adam E J. 1996. Ingestion of fluorescently labeled and phycoerythrin-containing prey by mixotrophic dinoflagellates. Aquat. Microb. Ecol., 10(2): 139–147.

Li J, Glibert P M, Zhou M. 2010. Temporal and spatial variability in nitrogen uptake kinetics during dinoflagellate blooms in the east China sea. Harmful Algae, 9(6): 531–539.

Li J, Glibert P M, Zhou M, Lu S, Lu D. 2009. Relationships between nitrogen and phosphorus forms and ratios and the development of dinoflagellate blooms in the East China Sea. Mar. Ecol. Prog. Ser., 383: 11–26.

Lindroth P, Mopper K. 1979. High-performance liquid-chromatographic determination of subpicomole amounts of amino-acids by precolumn fluorescence derivatization with ortho-phthaldialdehyde. Anal. Chem., 51(11): 1 667–1 674.

Litke D W. 1999. Review of phosphorus control measures in the united states and their effects on water quality, U.S. Geological Survey. U.S. Geological Survey, Water-Resources Investigations Report 99-4007, 38p.

Lomas M W, Glibert P M. 1999a. Interactions between NH +4 and NO uptake and assimilation: Comparison of diatoms and dinoflagellates at several growth temperatures. Mar. Biol., 133(3): 541–551.

Lomas M W, Glibert P M. 1999b. Temperature regulation of nitrate uptake: A novel hypothesis about nitrate uptake and reduction in cool-water diatoms. Limnol. Oceanogr., 44(3): 556–572.

Lomas M W, Glibert P M. 2000. Comparisons of nitrate uptake, storage, and reduction in marine diatoms and flagellates. J. Phycol., 36(5): 903–913.

Lu D D, Goebel J. 2001. Five red tide species in genus Prorocentrum including the description of Prorocentrum donghaiense Lu sp. nov. from the East China Sea. Chin. J. Oceanol. Limnol., 19(4): 337–344.

Maki A W, Porcella D B, Wendt R H. 1984. The impact of detergent phosphorus bans on receiving water-quality. Water Res., 18(7): 893–903.

Malone T C, Garside C, Haines K C, Roels O A. 1975. Nitrate uptake and growth of Chaetoceros sp. in large outdoor continuous cultures. Limnol. Oceanogr., 20(7): 9–19.

McDuff R E, Chisholm S W. 1982. The calculation of insitu growth-rates of phytoplankton populations from fractions of cells undergoing mitosis—a clarification. Limnol. Oceanogr., 27(4): 783–788.

Monod J. 1950. La technique de culture continue theorie et applications. Annales de l’Institut Pasteur, 79(4): 390–410.

NRC. 2000. Clean coastal waters: Understanding and reducing the effects of nutrient pollution. National Academies Press.

Paasche E, Bryceson I, Tangen K. 1984. Interspecific variation in dark nitrogen uptake by dinoflagellates. J. Phycol., 20(3): 394–401.

Paerl H W, Valdes L M, Joyner A R, Piehler M F. 2004. Solving problems resulting from solutions: Evolution of a dual nutrient management strategy for the eutrophying Neuse River estuary, North Carolina. Environ. Sci. Technol., 38(11): 3 068–3 073.

Phillips D J H, Tanabe S. 1989. Aquatic pollution in the Far-East. Mar. Pollut. Bull., 20(7): 297–303.

Revilla M, Alexander J, Glibert P M. 2005. Urea analysis in coastal waters: Comparison of enzymatic and direct methods. Limnol. and Oceanogr-Meth., 3: 290–299.

Rhee G Y. 1978. The continuous culture in phytoplankton ecology. In: Droop M R, Jannasch H W eds. Advances in Aquatic Microbiology. Academic Press, London. Vol.2, p.151–203.

Rhee G Y. 1973. Continuous culture study of phosphate uptake, growth-rate and polyphosphate in Scenedesmus sp. J. Phycol., 9(4): 495–506.

Rhee G Y, Gotham I J. 1981. The effect of environmental-factors on phytoplankton growth—light and the interactions of light with nitrate limitation. Limnol. Oceanogr., 26(4): 649–659.

Salerno M, Stoecker D K. 2009. Ectocellular glucosidase and peptidase activity of the mixotrophic dinoflagellate Prorocentrum minimum (dinophyceae). J. Phycol., 45(1): 34–45.

Sciandra A. 1991. Coupling and uncoupling between nitrate uptake and growth-rate in Prorocentrum minimum (dinophyceae) under different frequencies of pulsed nitrate supply. Mar. Ecol. Prog. Ser., 72(3): 261–269.

Sharfstein B, Roels O A, Harris V, Lee V. 1977. Effect of detergent legislation on phosphorus in effluent and receiving waters. J. Water Pollut. Control Fed., 49(9): 2 017–2 021.

Skipnes O, Eide I, Jensen A. 1980. Cage culture turbidostat—a device for rapid-determination of algal growth-rate. Appl. Environ. Microbiol., 40(2): 318–325.

Smayda T J. 1997. Harmful algal blooms: Their ecophysiology and general relevance to phytoplankton blooms in the sea. Limnol. Oceanogr., 42(5): 1 137–1 153.

Smayda T J. 2002. Adaptive ecology, growth strategies and the global bloom expansion of dinoflagellates. J. Oceanogr., 58(2): 281–294.

Stoecker D K, Gustafson D E. 2003. Cell-surface proteolytic activity of photosynthetic dinoflagellates. Aquat. Microb. Ecol., 30(2): 175–183.

Stoecker D K, Li A S, Coats D W, Gustafson D E, Nannen M K. 1997. Mixotrophy in the dinoflagellate Prorocentrum minimum. Mar. Ecol. Prog. Ser., 152(1–3): 1–12.

Suttle C A, Harrison P J. 1988. Ammonium and phosphate-uptake rates, N-P supply ratios, and evidence for N-limitation and P-limitation in some oligotrophic lakes. Limnol. Oceanogr., 33(2): 186–202.

Tyler M A, Seliger H H. 1978. Annual subsurface transport of a red tide dinoflagellate to its bloom area—water circulation patterns and organism distributions in Chesapeake Bay. Limnol. Oceanogr., 23(2): 227–246.

USDA. 2008. Fertilizer consumption and use—By year. United states department of agriculture. http://www.ers.usda.gov/Data/FertilizerUse/.

Vrede T, Ballantyne A, Mille-Lindblom C, Algesten G, Gudasz C, Lindahl S, Brunberg A K. 2009. Effects of N: P loading ratios on phytoplankton community composition, primary production and N fixation in a eutrophic lake. Freshw. Biol., 54(2): 331–344.

Watson T G. 1972. Present status and future prospects of turbidostat. J. Appl. Chem. Biotechnol. Abstr., 22(2): 229.

Yoshida T, Rao B S M, Ohasa S, Taguchi H. 1979. Dynamic analysis of a mixed culture in chemostat. J. Ferment. Technol., 57(6): 546–553.

Zhou M, Yan T, Zou J. 2003. Preliminary analysis of the characteristics of red tide areas in Changjiang River estuary and its adjacent sea. Chin. J. Appl. Ecol., 14(7): 1 031–1 038. (in Chinese)

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Effects of phytoplankton community and interaction between environmental variables on nitrogen uptake and transformations in an urban river

测试布局对涡轮效率的影响研究

液体火箭发动机背压振荡环境下的雾化特性研究进展

2.5D编织结构复合材料温度场特征研究

一种涡轮基组合动力的整机低速风洞试验研究