Purification and characterization of cold-active endo-1,4-β-glucanase produced by Pseudoalteromonas sp. AN545 from Antarctica

Jihong Shen; Guangfeng Kan; Cuijuan Shi; Zhenhuan Lei; Qiuju Xie; Wenjia Qian

doi:10.1007/s00343-011-0311-4

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Purification and characterization of cold-active endo-1,4-β-glucanase produced by Pseudoalteromonas sp. AN545 from Antarctica

OriginalPaper | Updated：2023-05-16

- Purification and characterization of cold-active endo-1,4-β-glucanase produced by Pseudoalteromonas sp. AN545 from Antarctica
- Purification and characterization of cold-active endo-1,4-β-glucanase produced by Pseudoalteromonas sp. AN545 from Antarctica
- 海洋湖沼学报（英文） 2011年29卷第5期页码：1086-1092
- Affiliations：
  
  1. Key Laboratory of Marine Bio-active Substances State Oceanic Administration
  2. School of the Ocean Harbin Institute of Technology at Weihai
- Author bio：
- Funds：
  
  Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2007AA091905), the Natural Science Foundation of Shandong Province (No. ZR2010DQ010), and the Fundamental Research Funds for the Central Universities (No. HIT. IBRSEM.2009148)
- DOI：10.1007/s00343-011-0311-4
  中图分类号：
- 网络首发：2011-08-08，
  
  纸质出版：2011
- Accepted：
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Purification and characterization of cold-active endo-1,4-β-glucanase produced by Pseudoalteromonas sp. AN545 from Antarctica[J]. 海洋湖沼学报（英文）, 2011,29(5):1086-1092.

Shen, J., Kan, G., Shi, C. et al. Purification and characterization of cold-active endo-1,4-β-glucanase produced by Pseudoalteromonas sp. AN545 from Antarctica., Chin. J. Ocean. Limnol. 29, 1086–1092 (2011). https://doi.org/10.1007/s00343-011-0311-4
Purification and characterization of cold-active endo-1,4-β-glucanase produced by Pseudoalteromonas sp. AN545 from Antarctica[J]. 海洋湖沼学报（英文）, 2011,29(5):1086-1092. DOI： 10.1007/s00343-011-0311-4.

Shen, J., Kan, G., Shi, C. et al. Purification and characterization of cold-active endo-1,4-β-glucanase produced by Pseudoalteromonas sp. AN545 from Antarctica., Chin. J. Ocean. Limnol. 29, 1086–1092 (2011). https://doi.org/10.1007/s00343-011-0311-4 DOI：

摘要

Abstract

A bacterium hydrolyzing carboxymethylcellulose

isolated from Antarctic sea ice

was identified as Pseudoalteromonas sp. based on 16S rDNA gene sequences and named as Pseudoalteromonas sp. AN545. The extracellular endo-1

4-β-glucanase AN-1 was purified successively by ammonium sulfate precipitation

DEAE-Sepharose ion exchange chromatography and Sephadex G-75 gel filtration chromatography. The molecular mass of AN-1 was estimated to be 47.5 kDa utilizing SDS-PAGE and gel chromatography analysis. AN-1 could hydrolyze caboxymethylcellulose

avicel and β-glucan

but not cellobiose

xylan and p-Nitrophenyl-β-D-glucopyranoside. The optimal temperature and pH for the β-glucanase activity of AN-1 were determined to be at 30°C and pH 6.0

respectively. AN-1 was stable at acidic solutions of pH 5.0–6.5 and temperatures below 30°C for 1 h. Moreover

the specific activity was enhanced by Ca2+ and Mg2+

and inhibited by Cu2+. The kinetic parameters Michaelis constant (K m) and maximum velocity (V max) of AN-1 were 3.96 mg/mL and 6.06×10−2 mg/(min·mL)

respectively.

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references

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