Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)

Jinxia Wang; Kuishuang Shao; Bin Cheng; Qinqin Lu; Baicheng Zhou

doi:10.1007/s00343-011-0295-0

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Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)

OriginalPaper | Updated：2023-05-16

- Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)
- Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)
- 海洋湖沼学报（英文） 2011年29卷第6期页码：1260-1266
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
  3. National Marine Environmental Monitoring Center
  4. Institute of Oceanology & Marine Fisheries
- Author bio：
- Funds：
  
  Supported by the National Special Research Fund for Non-Profit Sector (No. 200905020-06)
- DOI：10.1007/s00343-011-0295-0
  中图分类号：
- 网络首发：2011-10-25，
  
  纸质出版：2011
- Accepted：
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Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)[J]. 海洋湖沼学报（英文）, 2011,29(6):1260-1266.

Wang, J., Shao, K., Cheng, B. et al. Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)., Chin. J. Ocean. Limnol. 29, 1260–1266 (2011). https://doi.org/10.1007/s00343-011-0295-0
Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)[J]. 海洋湖沼学报（英文）, 2011,29(6):1260-1266. DOI： 10.1007/s00343-011-0295-0.

Wang, J., Shao, K., Cheng, B. et al. Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)., Chin. J. Ocean. Limnol. 29, 1260–1266 (2011). https://doi.org/10.1007/s00343-011-0295-0 DOI：

摘要

Abstract

A filamentous clone of Polysiphonia urceolata was regenerated from segments cut from the fronds of gametophytes. Unlike wild thalli with short virgate branchlets

the clone was filamentous with few branches. Many transparent trichoblasts arose from pericentral cells during the induction culture

but these were seldom observed during normal growth. The trichoblasts were uniseriate

often colorless

and formed lobed rhizoids rapidly when they came into contact with solid substrates. In addition to morphological characteristics

the photosynthetic properties and growth conditions of the clone differed from those of the mother plant. Cross-gradient light and temperature culture experiments revealed that the most favorable conditions for culture of the filamentous clone were 22°C and 95–120 μE/(m2·s) light intensity. The photosynthetic light saturation value for filaments was approx. 100 μE/(m2·s)

which is far lower than that of wild thalli. These results could be used to develop techniques for mass cultures of P. urceolata in photobioreactors for production of seed stock or bioactive products.

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references

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