Sequence polymorphism of two major histocompatibility (MH) class II B genes and their association with Vibrio anguillarum infection in half-smooth tongue sole (Cynoglossus semilaevis)

Chunmei Li; Quanqi Zhang; Yan Yu; Shuo Li; Qiwang Zhong; Yeying Sun; Zhigang Wang; Jie Qi; Jieming Zhai; Xubo Wang

doi:10.1007/s00343-011-0278-1

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Sequence polymorphism of two major histocompatibility (MH) class II B genes and their association with Vibrio anguillarum infection in half-smooth tongue sole (Cynoglossus semilaevis)

OriginalPaper | Updated：2023-05-16

- Sequence polymorphism of two major histocompatibility (MH) class II B genes and their association with Vibrio anguillarum infection in half-smooth tongue sole (Cynoglossus semilaevis)
- Sequence polymorphism of two major histocompatibility (MH) class II B genes and their association with Vibrio anguillarum infection in half-smooth tongue sole (Cynoglossus semilaevis)
- 海洋湖沼学报（英文） 2011年29卷第6期页码：1275-1286
- Affiliations：
  
  1. Key Laboratory of Marine Genetics and Breeding of Ministry of Education Ocean University of China
  2. Laizhou Ming Bo Aquatic Co. Ltd.
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30901098)
- DOI：10.1007/s00343-011-0278-1
  中图分类号：
- 网络首发：2011-10-25，
  
  纸质出版：2011
- Accepted：
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Sequence polymorphism of two major histocompatibility (MH) class II B genes and their association with Vibrio anguillarum infection in half-smooth tongue sole (Cynoglossus semilaevis)[J]. 海洋湖沼学报（英文）, 2011,29(6):1275-1286.

Li, C., Zhang, Q., Yu, Y. et al. Sequence polymorphism of two major histocompatibility (MH) class II B genes and their association with Vibrio anguillarum infection in half-smooth tongue sole (Cynoglossus semilaevis)., Chin. J. Ocean. Limnol. 29, 1275–1286 (2011). https://doi.org/10.1007/s00343-011-0278-1
Sequence polymorphism of two major histocompatibility (MH) class II B genes and their association with Vibrio anguillarum infection in half-smooth tongue sole (Cynoglossus semilaevis)[J]. 海洋湖沼学报（英文）, 2011,29(6):1275-1286. DOI： 10.1007/s00343-011-0278-1.

Li, C., Zhang, Q., Yu, Y. et al. Sequence polymorphism of two major histocompatibility (MH) class II B genes and their association with Vibrio anguillarum infection in half-smooth tongue sole (Cynoglossus semilaevis)., Chin. J. Ocean. Limnol. 29, 1275–1286 (2011). https://doi.org/10.1007/s00343-011-0278-1 DOI：

摘要

Abstract

Major histocompatibility complex (MHC) class II B molecules play an important role in the adaptive immune response in fish. Previous study has reported that two highly polymorphic class II B genes

Cyse-DAB and Cyse-DBB exist in half-smooth tongue sole (Cynoglossus semilaevis). In this study

the polymorphism within exon 2 of the class II B genes following bacterial challenge was evaluated. Two hundred C. semilaevis individuals were injected intraperitoneally with Vibrio anguillarum. Muscle tissue from the first 20 dead and 20 of the survivors was collected for genotyping. Sixty alleles from the 40 individuals were isolated

of which 32 belonged to Cyse-DAB and 28 belonged to Cyse-DBB. The rate of d N (non-synonymous substitution) was higher than that of d S (synonymous substitution) in the PBRs (peptide binding residues) of both class II B genes. Conversely

the rate of d S was higher than d N in the non-PBRs and the complete exon 2 sequence. Thus

the results suggest that positive selection has occurred in the PBRs and purifying selection in the non-PBRs and exon 2. Thirteen class II B alleles were used to study the association between alleles and resistance to infection. Though not significant

alleles Cyse-DAB*0601

Cyse-DAB*0706

and Cyse-DBB*0101

Cyse-DBB*1301 were only found in surviving individuals and may represent alleles that have resistance against V. anguillarum infection. Alleles Cyse-DAB*0701 and Cyse-DAB*1301 were significantly more prevalent in dead individuals than in surviving ones and may represent alleles that are associated with increased susceptibility to V. anguillarum infection.

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references

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