Development and application of antibody microarray for white spot syndrome virus detection in shrimp

Xiaoli Xu; Xiuzhen Sheng; Wenbin Zhan

doi:10.1007/s00343-011-0252-y

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Development and application of antibody microarray for white spot syndrome virus detection in shrimp

OriginalPaper | Updated：2023-05-16

- Development and application of antibody microarray for white spot syndrome virus detection in shrimp
- Development and application of antibody microarray for white spot syndrome virus detection in shrimp
- 海洋湖沼学报（英文） 2011年29卷第5期页码：930-941
- Affiliations：
  
  Laboratory of Pathology and Immunology of Aquatic Animals LMMEC Ocean University of China
- Author bio：
- Funds：
  
  Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2006AA100306) and Special Fund for Agro-Scientific Research in the Public Interest (No. 201103034)
- DOI：10.1007/s00343-011-0252-y
  中图分类号：
- 网络首发：2011-08-08，
  
  纸质出版：2011
- Accepted：
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Development and application of antibody microarray for white spot syndrome virus detection in shrimp[J]. 海洋湖沼学报（英文）, 2011,29(5):930-941.

Xu, X., Sheng, X. & Zhan, W. Development and application of antibody microarray for white spot syndrome virus detection in shrimp., Chin. J. Ocean. Limnol. 29, 930–941 (2011). https://doi.org/10.1007/s00343-011-0252-y
Development and application of antibody microarray for white spot syndrome virus detection in shrimp[J]. 海洋湖沼学报（英文）, 2011,29(5):930-941. DOI： 10.1007/s00343-011-0252-y.

Xu, X., Sheng, X. & Zhan, W. Development and application of antibody microarray for white spot syndrome virus detection in shrimp., Chin. J. Ocean. Limnol. 29, 930–941 (2011). https://doi.org/10.1007/s00343-011-0252-y DOI：

摘要

Abstract

Detecting white spot syndrome virus (WSSV) in shrimp in high efficiency and veracity is important for disease prevention in aquaculture. Antibody-based microarray is a novel proteomic technology that can meet the requirements. In this study

we developed an antibody microarray for WSSV-detection in a specific and parallel way at multiple samples. First

seven slides each with different modifications were characterized by atomic force microscope

and were compared in the efficiency of immobilizing proteins. Of the seven

3-dimensional structured agarose gel-modified slides were chosen appropriate for the microarray for having higher signal value and superior spot size. A purified rabbit anti-WSSV antibody was arrayed as the capture antibody of the microarray on the agarose gel-modified slides

and then the microarray slides were incubated in the tissue homogenate of sampled shrimp and the antibody-antigen complex was detected by Cy3-conjugated anti-WSSV monoclonal antibody. The results were measured by a laser chipscanner and analyzed with software. To obtain satisfied fluorescence signal intensity

optimal conditions were searched. The detection limit of the antibody microarray for WSSV is 0.62 μg/mL

with a proven long shelf life for 6 months at 4°C or 8 months at −20°C. Furthermore

concordance between antibody microarray and traditional indirect ELISA reached 100% for WSSV detection. These results suggest that the antibody microarray could be served as an effective tool for diagnostic and epidemiological studies of WSSV.

关键词

Keywords

references

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