Detection of 36 antibiotics in coastal waters using high performance liquid chromatography-tandem mass spectrometry

Guangshui Na; Jia Gu; Linke Ge; Peng Zhang; Zhen Wang; Chunyang Liu; Lin Zhang

doi:10.1007/s00343-011-0225-1

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Detection of 36 antibiotics in coastal waters using high performance liquid chromatography-tandem mass spectrometry

OriginalPaper | Updated：2023-05-16

- Detection of 36 antibiotics in coastal waters using high performance liquid chromatography-tandem mass spectrometry
- Detection of 36 antibiotics in coastal waters using high performance liquid chromatography-tandem mass spectrometry
- 海洋湖沼学报（英文） 2011年29卷第5期页码：1093-1102
- Affiliations：
  
  1. National Marine Environmental Monitoring Center
  2. School of Biology Technology Dalian Polytechnic University
- Author bio：
- Funds：
  
  Supported by Young Scientists Research Program (No. 2009507) and the Key Laboratory of Marine Bioactive Substances and Modern Analytical Techniques (No. MBSMAT-2010-04), SOA of China
- DOI：10.1007/s00343-011-0225-1
  中图分类号：
- 网络首发：2011-08-08，
  
  纸质出版：2011
- Accepted：
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Detection of 36 antibiotics in coastal waters using high performance liquid chromatography-tandem mass spectrometry[J]. 海洋湖沼学报（英文）, 2011,29(5):1093-1102.

Na, G., Gu, J., Ge, L. et al. Detection of 36 antibiotics in coastal waters using high performance liquid chromatography-tandem mass spectrometry., Chin. J. Ocean. Limnol. 29, 1093–1102 (2011). https://doi.org/10.1007/s00343-011-0225-1
Detection of 36 antibiotics in coastal waters using high performance liquid chromatography-tandem mass spectrometry[J]. 海洋湖沼学报（英文）, 2011,29(5):1093-1102. DOI： 10.1007/s00343-011-0225-1.

Na, G., Gu, J., Ge, L. et al. Detection of 36 antibiotics in coastal waters using high performance liquid chromatography-tandem mass spectrometry., Chin. J. Ocean. Limnol. 29, 1093–1102 (2011). https://doi.org/10.1007/s00343-011-0225-1 DOI：

摘要

Abstract

Among pharmaceuticals and personal care products released into the aquatic environment

antibiotics are of particular concern

because of their ubiquity and health effects. Although scientists have recently paid more attention to the threat of antibiotics to coastal ecosystems

researchers have often focused on relatively few antibiotics

because of the absence of suitable analytical methods. We have therefore developed a method for the rapid detection of 36 antibiotic residues in coastal waters

including tetracyclines (TCs)

sulfanilamides (SAs)

and quinolones (QLs). The method consists of solid-phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis

using electrospray ionization (ESI) in positive mode. The SPE was performed with Oasis HLB and Oasis MCX cartridges. Chromatographic separation on a C18 column was achieved using a binary eluent containing methanol and water with 0.1% formic acid. Typical recoveries of the analytes ranged from 67.4% to 109.3% at a fortification level of 100 ng/L. The precision of the method

calculated as relative standard deviation (RSD)

was below 14.6% for all the compounds. The limits of detection (LODs) varied from 0.45 pg to 7.97 pg. The method was applied to determine the target analytes in coastal waters of the Yellow Sea in Liaoning

China. Among the tested antibiotics

31 were found in coastal waters

with their concentrations between the LOD and 212.5 ng/L. These data indicate that this method is valid for analysis of antibiotics in coastal waters. The study first reports such a large number of antibiotics along the Yellow Sea coast of Liaoning

and should facilitate future comprehensive evaluation of antibiotics in coastal ecosystems.

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references

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