The loss of genetic diversity during captive breeding of the endangered sculpin, Trachidermus fasciatus, based on ISSR markers: implications for its conservation

Xiaoxiao Bi; Qiaoli Yang; Tianxiang Gao; Chuangju Li

doi:10.1007/s00343-011-0185-5

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The loss of genetic diversity during captive breeding of the endangered sculpin, Trachidermus fasciatus, based on ISSR markers: implications for its conservation

OriginalPaper | Updated：2023-05-16

- The loss of genetic diversity during captive breeding of the endangered sculpin, Trachidermus fasciatus, based on ISSR markers: implications for its conservation
- The loss of genetic diversity during captive breeding of the endangered sculpin, Trachidermus fasciatus, based on ISSR markers: implications for its conservation
- 海洋湖沼学报（英文） 2011年29卷第5期页码：958-966
- Affiliations：
  
  1. Fisheries College Ocean University of China
  2. Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences
- Author bio：
- Funds：
  
  Supported by the Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture (No. LFBCU0713), and the Special Research Foundation for Public Welfare Marine Program (No. 200905019-2);YANG Qiaoli contributed equally to this work
- DOI：10.1007/s00343-011-0185-5
  中图分类号：
- 网络首发：2011-08-08，
  
  纸质出版：2011
- Accepted：
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The loss of genetic diversity during captive breeding of the endangered sculpin, Trachidermus fasciatus, based on ISSR markers: implications for its conservation[J]. 海洋湖沼学报（英文）, 2011,29(5):958-966.

Bi, X., Yang, Q., Gao, T. et al. The loss of genetic diversity during captive breeding of the endangered sculpin, Trachidermus fasciatus, based on ISSR markers: implications for its conservation., Chin. J. Ocean. Limnol. 29, 958–966 (2011). https://doi.org/10.1007/s00343-011-0185-5
The loss of genetic diversity during captive breeding of the endangered sculpin, Trachidermus fasciatus, based on ISSR markers: implications for its conservation[J]. 海洋湖沼学报（英文）, 2011,29(5):958-966. DOI： 10.1007/s00343-011-0185-5.

Bi, X., Yang, Q., Gao, T. et al. The loss of genetic diversity during captive breeding of the endangered sculpin, Trachidermus fasciatus, based on ISSR markers: implications for its conservation., Chin. J. Ocean. Limnol. 29, 958–966 (2011). https://doi.org/10.1007/s00343-011-0185-5 DOI：

摘要

Abstract

Inter-simple sequence repeat (ISSR) markers were used to determine the genetic variation and genetic differentiation of cultured and wild populations of Trachidermus fasciatus

an endangered catadromous fish species in China. Six selected primers were used to amplify DNA samples from 85 individuals

and 353 loci were detected. Relatively low genetic diversity was detected in the cultured population (the percentage of polymorphic loci PPL = 73.80%

Nei’s gene diversity h = 0.178 2

Shannon information index I = 0.276 9). However

the genetic diversity at the species level was relatively high (PPL = 91.78%; h = 0.258 3

I = 0.398 6). The UPGMA tree grouped together the genotypes almost according to their cultured and wild origin

showing distinct differences in genetic structure between wild and cultured populations. The pairwise F st values confirmed significant genetic differentiation between wild and cultured samples. The cultivated population seems to be low in genetic diversity as a result of detrimental genetic effects in the captive population. The results suggest that ISSR markers are effective for rapid assessment of the degree of diversity of a population

thus giving important topical information relevant to preserving endangered species.

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references

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