In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica

Jing Wang; Quanbin Zhang; Zhongshan Zhang; Yun Hou; Hong Zhang

doi:10.1007/s00343-011-0181-9

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In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica

OriginalPaper | Updated：2023-05-16

- In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica
- In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica
- 海洋湖沼学报（英文） 2011年29卷第3期页码：679-685
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the Ocean Public Welfare Scientific Research Project, State Oceanic Administration of the People’s Republic of China (No. 201005024)
- DOI：10.1007/s00343-011-0181-9
  中图分类号：
- 网络首发：2011-05-15，
  
  纸质出版：2011
- Accepted：
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In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica[J]. 海洋湖沼学报（英文）, 2011,29(3):679-685.

Wang, J., Zhang, Q., Zhang, Z. et al. In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica, ., Chin. J. Ocean. Limnol. 29, 679–685 (2011). https://doi.org/10.1007/s00343-011-0181-9
In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica[J]. 海洋湖沼学报（英文）, 2011,29(3):679-685. DOI： 10.1007/s00343-011-0181-9.

Wang, J., Zhang, Q., Zhang, Z. et al. In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica, ., Chin. J. Ocean. Limnol. 29, 679–685 (2011). https://doi.org/10.1007/s00343-011-0181-9 DOI：

摘要

Abstract

Fucoidan

a group of sulfated heteropolysaccharides

was extracted from Laminaria japonica

an important economic alga species in China. The anticoagulant activity of fucoidan and its derivatives (including sulfated

phosphorylated

and aminated fucoidan) was examined using in-vitro anticoagulant systems. The correlation between chemical variations within the fucoidan group and anticoagulant activity was determined. The in-vitro anticoagulant properties of fucoidan and its derivatives were determined by measuring activated partial thromboplastin time (APTT)

prothrombin time (PT)

and thrombin time (TT). The results indicate anticoagulant activity in all samples using APTT and TT assays; however

only the fucoidan derivatives affected the PT assay. Thus

the fucoidan derivatives were able to inhibit both intrinsic and extrinsic blood coagulants. Fucoidan (FPS) and its derivatives presented better anticoagulant activity than low molecular weight fucoidan (DFPS) and its derivatives

suggesting that molecular weight and proper conformation are contributing factors for anticoagulant activity of polysaccharides. Amino groups have a positive charge and can thus change the charge density of fucoidan. Accordingly

among the tested samples

aminated fucoidan (NF) was the most active reflecting the importance of charge density for anticoagulant activity. Available data obtained using in-vitro models suggest that the sulfate content

sulfate/total-sugar ratio

molecular weight

and the substituted group of fucoidan are important factors for anticoagulant activity but that the influence of sulfate

phosphate and amino groups on anticoagulant activity was different.

关键词

Keywords

references

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