Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis
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Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis
Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis
海洋湖沼学报(英文)2011年29卷第3期 页码:609-615
Affiliations:
1. Inspection & Quarantine Technology Center Shandong Entry-Exit Inspection & Quarantine Bureau
2. Jiangsu Key Laboratory of Marine Biotechnology Huaihai Institute of Technology
3. College of Chemical Engineering Qingdao University of Science & Technology
4. Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
Author bio:
Funds:
Supported by the Open Funds of Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology (No. 2007HS015), the Shandong Post-Doctoral Science Foundation (No. 200802039), and the China Postdoctoral Science Foundation (No. 20060390999)
Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis[J]. 海洋湖沼学报(英文), 2011,29(3):609-615.
Liu, Y., Li, J. & Ye, N. Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis, ., Chin. J. Ocean. Limnol. 29, 609–615 (2011). https://doi.org/10.1007/s00343-011-0167-7
Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis[J]. 海洋湖沼学报(英文), 2011,29(3):609-615. DOI: 10.1007/s00343-011-0167-7.
Liu, Y., Li, J. & Ye, N. Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis, ., Chin. J. Ocean. Limnol. 29, 609–615 (2011). https://doi.org/10.1007/s00343-011-0167-7DOI:
Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis
摘要
Abstract
The genetic diversity of wild and hatchery populations of half-smooth tongue sole Cynoglossus semilaevis
based on observation of amplified fragment length polymorphism (AFLP) was described. Two hundred individuals from four wild populations
Laizhou (LZ)
Weihai (WH)
Qingdao (QD)
Rizhao (RZ)
and one hatchery population
Mingbo (MB)
were screened using eight different AFLP primer combinations. A total of 384 loci were screened in the five studied populations. 48.4%
51.3%
50.7%
49.3% and 45.8% of these loci were polymorphic among the individuals tested in the LZ
WH
QD
RZ and MB populations
respectively. The number of polymorphic loci detected by single primer combinations ranged from 17 to 35. The average heterozygosity of the LZ
WH
QD
RZ and MB populations was 0.072
0.093
0.092
0.090 and 0.063
respectively. The WH population showed the highest genetic diversity in terms of total number of AFLP bands
total number of polymorphic bands
average heterozygosity and percentage of low frequency (0–0.2) polymorphic loci among all the populations
while the LZ population was the lowest among the wild populations. Compared with the wild populations
the hatchery population showed a low genetic viability.
关键词
Keywords
references
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相关机构
Institute of Oceanology
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