Transcriptional changes in epigenetic modifiers associated with gene silencing in the intestine of the sea cucumber, Apostichopus japonicus (Selenka), during aestivation

Tianming Wang; Hongsheng Yang; Huan Zhao; Muyan Chen; Bing Wang

doi:10.1007/s00343-011-0143-2

Your Location：

Home >

Browse articles >

Transcriptional changes in epigenetic modifiers associated with gene silencing in the intestine of the sea cucumber, Apostichopus japonicus (Selenka), during aestivation

OriginalPaper | Updated：2023-05-16

- Transcriptional changes in epigenetic modifiers associated with gene silencing in the intestine of the sea cucumber, Apostichopus japonicus (Selenka), during aestivation
- Transcriptional changes in epigenetic modifiers associated with gene silencing in the intestine of the sea cucumber, Apostichopus japonicus (Selenka), during aestivation
- 海洋湖沼学报（英文） 2011年29卷第6期页码：1267-1274
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Key Technology Research and Development Program (No. 2006AA10A411), and the Agricultural Seed Project of Shandong Province
- DOI：10.1007/s00343-011-0143-2
  中图分类号：
- 网络首发：2011-10-25，
  
  纸质出版：2011
- Accepted：
Scan QR Code
Transcriptional changes in epigenetic modifiers associated with gene silencing in the intestine of the sea cucumber, Apostichopus japonicus (Selenka), during aestivation[J]. 海洋湖沼学报（英文）, 2011,29(6):1267-1274.

Wang, T., Yang, H., Zhao, H. et al. Transcriptional changes in epigenetic modifiers associated with gene silencing in the intestine of the sea cucumber, Apostichopus japonicus (Selenka), during aestivation., Chin. J. Ocean. Limnol. 29, 1267–1274 (2011). https://doi.org/10.1007/s00343-011-0143-2
Transcriptional changes in epigenetic modifiers associated with gene silencing in the intestine of the sea cucumber, Apostichopus japonicus (Selenka), during aestivation[J]. 海洋湖沼学报（英文）, 2011,29(6):1267-1274. DOI： 10.1007/s00343-011-0143-2.

Wang, T., Yang, H., Zhao, H. et al. Transcriptional changes in epigenetic modifiers associated with gene silencing in the intestine of the sea cucumber, Apostichopus japonicus (Selenka), during aestivation., Chin. J. Ocean. Limnol. 29, 1267–1274 (2011). https://doi.org/10.1007/s00343-011-0143-2 DOI：

摘要

Abstract

The sea cucumber

Apostichopus japonicus

undergoes aestivation to improve survival during periods of high-temperature. During aestivation

the metabolic rate is depressed to reduce the consumption of reserved energy. We evaluated the role of epigenetic modification on global gene silencing during metabolic rate depression in the sea cucumber. We compared the expression of epigenetic modifiers in active and aestivating sea cucumbers. The expression of three genes involved in DNA methylation and chromatin remodeling (DNA (cytosine-5)-methyltransferase 1

Methyl-CpG-binding domain protein 2)

and Chromodomain-helicase-DNA-binding protein 5) was significantly higher during aestivation (Days 20 and 40). Similarly

we observed an increase in the expression of genes involved in histone acetylation (Histone deacetylase 3) and Histone-binding protein RBBP4) during the early (Days 5 and 10) and late phases (Days 20 and 40) of aestivation. There was no change in the expression of KAT2B

a histone acetyltransferase. However

the expression of histone methylation associated modifiers (Histone-arginine methyltransferase CARMER and Histone-lysine N-methyltransferase MLL5) was significantly higher after 5 d in the aestivating group. The results suggest that the expression of epigenetic modifiers involved in DNA methylation

chromatin remodeling

histone acetylation

and histone methylation is upregulated during aestivation. We hypothesize that these changes regulate global gene silencing during aestivation in A. japonicus.

关键词

Keywords

references

Blanco J C G, Minucci S, Lu J M, Yang X J, Walker K K, Chen H W, Evans R M, Nakatani Y, Ozato K. 1998. The histone acetylase PCAF is a nuclear receptor coactivator. Genes & Development, 12(11): 1 638–1 651.

De Ruijter A J M, Van Gennip A H, Caron H N, Kemp S, Van Kuilenburg A B P. 2003. Histone deacetylases (HDACs): characterization of the classical HDAC family. Biochemical Journal, 370: 737–749.

Gao F, Yang H S, Xu Q, Wang F Y, Liu G B. 2009. Effect of water temperature on digestive enzyme activity and gut mass in sea cucumber Apostichopus japonicus (Selenka), with special reference to aestivation. Chinese Journal of Oceanology and Limnology, 27(4): 714–722.

Hudson N J, Lehnert S A, Ingham A B, Symonds B, Franklin C E, Harper G S. 2006. Lessons from an estivating frog: sparing muscle protein despite starvation and disuse. American Journal of Physiology-Regulatory Integrative and Comparative Physiology, 290(3): R836–R843.

Hudson N J, Lonhienne T G A, Franklin C E, Harper G S, Lehnert S A. 2008. Epigenetic silencers are enriched in dormant desert frog muscle. Journal of Comparative Physiology B-Biochemical Systemic and Environmental Physiology, 178(6): 729–734.

Jeong S J, Lu H X, Cho W K, Park H U, Pise-Masison C, Brady J N. 2006. Coactivator-associated arginine methyltransferase 1 enhances transcriptional activity of the human T-cell lymphotropic virus type 1 long terminal repeat through direct interaction with tax. Journal of Virology, 80(20): 10 036–10 044.

Ji T T, Dong Y W, Dong S L. 2008. Growth and physiological responses in the sea cucumber, Apostichopus japonicus Selenka: Aestivation and temperature. Aquaculture, 283(1–4): 180–187.

Kouzarides T. 2002. Histone methylation in transcriptional control. Current Opinion in Genetics & Development, 12(2): 198–209.

Larade K, Storey K B. 2002. A profile of the metabolic responses to anoxia in marine invertebrates. In: Storey K B, Storey J M eds. Sensing, Signaling and Cell Adaptation. Elsevier Science, Amsterdam. p.27–46.

Lee D Y, Teyssier C, Strahl B D, Stallcup M R. 2005. Role of protein methylation in regulation of transcription. Endocrine Reviews, 26(2): 147–170.

Liu Y, Li F, Song B, Sun H, Zhang X, Gu B. 1996. Study on aestivating habit of sea cucumber Apostichopus japonicus Selenka: 1. Ecological characteristic of aestivation. Journal of Fishery Sciences of China, 3: 41–48. (in Chinese with English abstract)

Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods, 25(4): 402–408.

Morin P J, Storey K B. 2006. Evidence for a reduced transcriptional state during hibernation in ground squirrels. Cryobiology, 53(3): 310–318.

Morin P J, Storey K B. 2009. Mammalian hibernation: differential gene expression and novel application of epigenetic controls. International Journal of Developmental Biology, 53(2–3): 433–442.

Newell-Price J, Clark A J L, King P. 2000. DNA methylation and silencing of gene expression. Trends in Endocrinology and Metabolism, 11(4): 142–148.

Ng H H, Zhang Y, Hendrich B, Johnson C A, Turner B M, Erdjument-Bromage H, Tempst P, Reinberg D, Bird A. 1999. MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex. Nature Genetics, 23(1): 58–61.

Nicolas E, Ait-Si-Ali S, Trouche D. 2001. The histone deacetylase HDAC3 targets RbAp48 to the retinoblastoma protein. Nucleic Acids Research, 29(15): 3 131–3 136.

Okuda M, Horikoshi M, Nishimura Y. 2007. Structural polymorphism of chromodomains in Chd1. Journal of Molecular Biology, 365(4): 1 047–1 062.

Osborne P G, Gao B, Hashimoto M. 2004. Determination in vivo of newly synthesized gene expression in hamsters during phases of the hibernation cycle. Japanese Journal of Physiology, 54(3): 295–305.

Robertson K D, Ait-Si-Ali S, Yokochi T, Wade P A, Jones P L, Wolffe A P. 2000. DNMT1 forms a complex with Rb, E2F1 and HDAC1 and represses transcription from E2F-responsive promoters. Nature Genetics, 25(3): 338–342.

Sebastian S, Sreenivas P, Sambasivan R, Cheedipudi S, Kandalla P, Pavlath G K, Dhawan J. 2009. MLL5, a trithorax homolog, indirectly regulates H3K4 methylation, represses cyclin A2 expression, and promotes myogenic differentiation. Proceedings of the National Academy of Sciences of the United States of America, 106(12): 4 719–4 724.

Storey K B, Storey J M. 2004. Metabolic rate depression in animals: transcriptional and translational controls. Biological Reviews of the Cambridge Philosophical Society, 79(1): 207–233.

Storey K B, Storey J M. 2007. Tribute to P. L. Lutz: putting life on’ pause’-molecular regulation of hypometabolism. Journal of Experimental Biology, 210(Pt 10): 1 700–1 714.

Storey K B, Storey J M. 2010. Metabolic regulation and gene expression during aestivation. Progress in Molecular and Subcellular Biology, 49: 25–45.

Tate P H, Bird A P. 1993. Effects of DNA methylation on DNA-binding proteins and gene expression. Current Opinion in Genetics & Development, 3(2): 226–231.

Thompson P M, Gotoh T, Kok M, White P S, Brodeur G M. 2003. CHD5, a new member of the chromodomain gene family, is preferentially expressed in the nervous system. Oncogene, 22(7): 1 002–1 011.

van Breukelen F, Martin S L. 2002. Reversible depression of transcription during hibernation. J. Comp. Physiol. B, 172: 355–361.

Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. 2002. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology, 3(7): research 0034.1–0034.11.

Wade P A, Gegonne A, Jones P L, Ballestar E, Aubry F, Wolffe A P. 1999. Mi-2 complex couples DNA methylation to chromatin remodeling and histone deacetylation. Nature Genetics, 23(1): 62–66.

Yang H S, Yuan X T, Zhou Y, Mao Y Z, Zhang T, Liu Y. 2005. Effects of body size and water temperature on food consumption and growth in the sea cucumber Apostichopus japonicus (Selenka) with special reference to aestivation. Aquaculture Research, 36(11): 1 085–1 092.

Yang H S, Zhou Y, Zhang T, Yuan X T, Li X X, Liu Y, Zhang F S. 2006. Metabolic characteristics of sea cucumber Apostichopus japonicus (Selenka) during aestivation. Journal of Experimental Marine Biology and Ecology, 330(2): 505–510.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Effect of water temperature on digestive enzyme activity and gut mass in sea cucumber Apostichopus japonicus (Selenka), with special reference to aestivation

Analysis of fatty acid composition of sea cucumber Apostichopus japonicus using multivariate statistics

Selection of reference genes for qRT-PCR analysis of gene expression in sea cucumber Apostichopus japonicus during aestivation

Seasonal changes in gut microbiota of sea cucumber over natural aestivation cycle

Expression profiles of sex-related genes in gonads of genetic male Takifugu rubripes after 17β-estradiol immersion