Glutamate dehydrogenase and Na+-K+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels

Erchao Li; Leticia Arena; Gabriel Lizama; Gabriela Gaxiola; Gerard Cuzon; Carlos Rosas; Liqiao Chen; Alain Van Wormhoudt

doi:10.1007/s00343-011-0093-8

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Glutamate dehydrogenase and Na+-K+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels

OriginalPaper | Updated：2023-05-16

- Glutamate dehydrogenase and Na+-K+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels
- Glutamate dehydrogenase and Na+-K+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels
- 海洋湖沼学报（英文） 2011年29卷第2期页码：343-349
- Affiliations：
  
  1. School of Life Science East China Normal University
  2. UMR Station de Biologie Marine du Muséum National d’Histoire Naturelle,5178
  3. Unidad Multidisciplinaria de Docencia e Investigaciòn Facultad de Ciencias Universidad Nacional Autónoma de México Hunucmá
  4. IFREMER
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (Nos. 31001098 and 30771670), the National High Technology R&D Program (863 Program) (No. 2006BAD01A13), the National Basic Research Program of China (973 Program) (No. 2009CB118702), Shanghai Committee of Science and Technology, China (Nos. 08DZ1906401, 09ZR1409800, 10JC1404100), Shanghai Agriculture Science and Technology Key Grant (No.2-1, 2009), the Specialized Research Fund for the Doctoral Program of Higher Education of China (No. 200802690012), and partially by the E-Institute of Shanghai Municipal Education Commission (No. E03009), and the Key and Open Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture
- DOI：10.1007/s00343-011-0093-8
  中图分类号：
- 网络首发：2011-03-02，
  
  纸质出版：2011
- Accepted：
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Glutamate dehydrogenase and Na+-K+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels[J]. 海洋湖沼学报（英文）, 2011,29(2):343-349.

Li, E., Arena, L., Lizama, G. et al. Glutamate dehydrogenase and Na+-K+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels., Chin. J. Ocean. Limnol. 29, 343–349 (2011). https://doi.org/10.1007/s00343-011-0093-8
Glutamate dehydrogenase and Na+-K+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels[J]. 海洋湖沼学报（英文）, 2011,29(2):343-349. DOI： 10.1007/s00343-011-0093-8.

Li, E., Arena, L., Lizama, G. et al. Glutamate dehydrogenase and Na+-K+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels., Chin. J. Ocean. Limnol. 29, 343–349 (2011). https://doi.org/10.1007/s00343-011-0093-8 DOI：

摘要

Abstract

Improvement in the osmoregulation capacity via nutritional supplies is vitally important in shrimp aquaculture. The effects of dietary protein levels on the osmoregulation capacity of the Pacific white shrimp (L. vannamei) were investigated. This involved an examination of growth performance

glutamate dehydrogenase (GDH) and Na+-K+ ATPase mRNA expression

and GDH activity in muscles and gills. Three experimental diets were formulated

containing 25%

40%

and 50% dietary protein

and fed to the shrimp at a salinity of 25. After 20 days

no significant difference was observed in weight gain

though GDH and Na+-K+ ATPase gene expression and GDH activity increased with higher dietary protein levels. Subsequently

shrimp fed diets with 25% and 50% dietary protein were transferred into tanks with salinities of 38 and 5

respectively

and sampled at weeks 1 and 2. Shrimp fed with 40% protein at 25 in salinity (optimal conditions) were used as a control. Regardless of the salinities

shrimp fed with 50% dietary protein had significantly higher growth performance than other diets; no significant differences were found in comparison with the control. Shrimp fed with 25% dietary protein and maintained at salinities of 38 and 5 had significantly lower weight gain values after 2 weeks. Ambient salinity change also stimulated the hepatosomatic index

which increased in the first week and then recovered to a relatively normal level

as in the control

after 2 weeks. These findings indicate that in white shrimp

the specific protein nutrient and energy demands related to ambient salinity change are associated with protein metabolism. Increased dietary protein level could improve the osmoregulation capacity of L. vannamei with more energy resources allocated to GDH activity and expression.

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