Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3

Shiying Ren; Xiangqian Li; Jianbo Jia; Fei Liu; Tian Xiao

doi:10.1007/s00343-011-0071-1

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Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3

OriginalPaper | Updated：2023-05-16

- Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3
- Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3
- 海洋湖沼学报（英文） 2011年29卷第5期页码：917-921
- Affiliations：
  
  1. School of Life Science and Chemical Engineering Huaiyin Institute of Technology
  2. Key Laboratory of Marine Ecology and Environmental Sciences Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Basic Research Program of China (973 Program) (No. 2006AA10A401) and the National Natural Science Foundation of China (No. 40376048)
- DOI：10.1007/s00343-011-0071-1
  中图分类号：
- 网络首发：2011-08-08，
  
  纸质出版：2011
- Accepted：
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Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3[J]. 海洋湖沼学报（英文）, 2011,29(5):917-921.

Ren, S., Li, X., Jia, J. et al. Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3., Chin. J. Ocean. Limnol. 29, 917–921 (2011). https://doi.org/10.1007/s00343-011-0071-1
Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3[J]. 海洋湖沼学报（英文）, 2011,29(5):917-921. DOI： 10.1007/s00343-011-0071-1.

Ren, S., Li, X., Jia, J. et al. Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3., Chin. J. Ocean. Limnol. 29, 917–921 (2011). https://doi.org/10.1007/s00343-011-0071-1 DOI：

摘要

Abstract

Hydantoinase is involved in the production of optically pure amino acids from racemic 5-mono-substituted hydantoins. We measured the D-hydantoinase activity in marine Halomonas sp. YSR-3 and amplified the D-hydantoinase gene by PCR. The gene was inserted into vector pGM-T and transformed into E. coli TOP10. The positive transformants with the D-hydantoinase gene were sequenced. The sequenced fragment comprises 1 510 base pairs. The D-hydantoinase gene from YSR-3 is 77% similar to that from Pseudomonas entomophila L4 by searching against the NCBI databse. The protein product of the YSR-3 D-hydantoinase gene is 75%

73%

and 70% similar to those from Pseudomonas fluorescens Pf-5

Marinomonas sp. MED121

and Burkholderia vietnamiensis G4

respectively. The difference of the D-hydantoinase gene between marine Halomonas sp. YSR-3 and other terrestrial organisms is distinct.

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references

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