Culture techniques and growth characteristics of Dinophysis acuminata and its prey

Mengmeng Tong; Qixing Zhou; Kulis M. David; Tianjiu Jiang; Yuzao Qi; Anderson M. Donald

doi:10.1007/s00343-010-9960-y

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Culture techniques and growth characteristics of Dinophysis acuminata and its prey

OriginalPaper | Updated：2023-05-16

- Culture techniques and growth characteristics of Dinophysis acuminata and its prey
- Culture techniques and growth characteristics of Dinophysis acuminata and its prey
- 海洋湖沼学报（英文） 2010年28卷第6期页码：1230-1239
- Affiliations：
  
  1. Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education) College of Environmental Science and Engineering Nankai University
  2. Woods Hole Oceanographic Institution
  3. Research Center for Red Tide and Aquatic Environments Jinan University
- Author bio：
- Funds：
  
  Support to M. TONG is provided by a grand fostering project from the Ministry of Education, People’s Republic of China (No. 707011) and the China Scholarship Council (CSC) 2007. Research is also supported by grants (to ANDERSON M. Donald) from the U.S. National Science Foundation (Nos. NSF OCE-0402707;OCE-0850421) and from the Ocean Life Institute at the Woods Hole Oceanographic Institution
- DOI：10.1007/s00343-010-9960-y
  中图分类号：
- 网络首发：2010-11-10，
  
  纸质出版：2010
- Accepted：
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Culture techniques and growth characteristics of Dinophysis acuminata and its prey[J]. 海洋湖沼学报（英文）, 2010,28(6):1230-1239.

Tong, M., Zhou, Q., David, K.M. et al. Culture techniques and growth characteristics of Dinophysis acuminata and its prey., Chin. J. Ocean. Limnol. 28, 1230–1239 (2010). https://doi.org/10.1007/s00343-010-9960-y
Culture techniques and growth characteristics of Dinophysis acuminata and its prey[J]. 海洋湖沼学报（英文）, 2010,28(6):1230-1239. DOI： 10.1007/s00343-010-9960-y.

Tong, M., Zhou, Q., David, K.M. et al. Culture techniques and growth characteristics of Dinophysis acuminata and its prey., Chin. J. Ocean. Limnol. 28, 1230–1239 (2010). https://doi.org/10.1007/s00343-010-9960-y DOI：

摘要

Abstract

The dinoflagellate Dinophysis acuminata Claparede & Lachmann is a toxic alga that causes diarrhetic shellfish poisoning. No Dinophysis species were maintained in culture for a long period of time until 2006 when Park successfully established D. acuminata in culture using a three-step feeding protocol in which the cryptophyte

Geminigera cryophila

is fed to Myrionecta rubra (=Mesodinium rubrum)

a ciliate that is in turn fed to D. acuminata. In this paper

we present the details of culturing D. acuminata from the Northeastern United States. The protocols described herein can be adopted for laboratory studies of this species. The effects of temperature on the growth and ingestion rates of D. acuminata were also examined. The results show that D. acuminata growth rate was 0.23 /d at 10°C and 0.11 /d at 4°C when fed M. rubra prey. The maximum prey ingestion rate was 2.80 Dinophysis cell/d at 10°C

although the rate decreased slightly at 4°C. In overall

temperature showed a greater influence on growth rate of D. acuminata than on the ingestion rate under the study conditions

and the quantity of available food was also an important regulator to D. acuminata growth.

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references

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