Phytoplankton diversity in the East China Sea and Yellow Sea measured by PCR-DGGE and its relationships with environmental factors

Jing Sun; Zhigang Yu; Yahui Gao; Qianqian Zhou; Yu Zhen; Hongtao Chen; Liyuan Zhao; Qingzhen Yao; Tiezhu Mi

doi:10.1007/s00343-010-9285-x

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Phytoplankton diversity in the East China Sea and Yellow Sea measured by PCR-DGGE and its relationships with environmental factors

OriginalPaper | Updated：2023-05-16

- Phytoplankton diversity in the East China Sea and Yellow Sea measured by PCR-DGGE and its relationships with environmental factors
- Phytoplankton diversity in the East China Sea and Yellow Sea measured by PCR-DGGE and its relationships with environmental factors
- 海洋湖沼学报（英文） 2010年28卷第2期页码：315-322
- Affiliations：
  
  1. College of Marine Life Sciences Ocean University of China
  2. Key Laboratory of Marine Chemistry Theory and Technology Ministry of Education Ocean University of China
  3. School of Life Sciences Xiamen University
  4. Key Laboratory of Marine Environment and Ecology Ministry of Education Ocean University of China
- Author bio：
- Funds：
  
  Supported by the National High Technology Research and Development Program of China (863 Program) (No.2006AA09Z178), the National Natural Science Foundation of China (No.30490232), Water Pollution Control and Treatment-National Science and Technology Special Grand (No.2008ZX07526-003), and the National Basic Research Program of China (973 Program) (No.2005CB422305)
- DOI：10.1007/s00343-010-9285-x
  中图分类号：
- 网络首发：2010-03-20，
  
  纸质出版：2010
- Accepted：
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Phytoplankton diversity in the East China Sea and Yellow Sea measured by PCR-DGGE and its relationships with environmental factors[J]. 海洋湖沼学报（英文）, 2010,28(2):315-322.

Sun, J., Yu, Z., Gao, Y. et al. Phytoplankton diversity in the East China Sea and Yellow Sea measured by PCR-DGGE and its relationships with environmental factors., Chin. J. Ocean. Limnol. 28, 315–322 (2010). https://doi.org/10.1007/s00343-010-9285-x
Phytoplankton diversity in the East China Sea and Yellow Sea measured by PCR-DGGE and its relationships with environmental factors[J]. 海洋湖沼学报（英文）, 2010,28(2):315-322. DOI： 10.1007/s00343-010-9285-x.

Sun, J., Yu, Z., Gao, Y. et al. Phytoplankton diversity in the East China Sea and Yellow Sea measured by PCR-DGGE and its relationships with environmental factors., Chin. J. Ocean. Limnol. 28, 315–322 (2010). https://doi.org/10.1007/s00343-010-9285-x DOI：

摘要

Abstract

Relationships between phytoplankton community composition and environmental variables in the East China Sea (ECS) and Yellow Sea (YS) were investigated using geochemical and molecular microbiology methods. The diversity of phytoplankton was characterized using cultivation-independent PCR-based denaturing gradient gel electrophoresis (DGGE). Groups resulting from unweighted pair-group method with arithmetic averages clustering of the DGGE profiles showed good consistency with the eco-environmental characteristics of the sea area they belonged to. Additionally

the clustering results based on DGGE fingerprinting and those based on morphological compositions were practically identical. The relationship of phytoplankton diversity to environmental factors was statistically analyzed. Temperature

dissolved inorganic nitrogen (DIN)

and silicate-Si were found significantly related to the phytoplankton community composition. Canonical correspondence analysis (CCA) was performed to reveal the relationship between community composition and these three environmental factors. Generally

values of the ECS are clearly separated from those of the YS in the CCA biplot

due to mainly the effect of temperature and DIN.

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