Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease

Zhaolan Mo; Dongsheng Guo; Yunxiang Mao; Xuhong Ye; Yuxia Zou; Peng Xiao; Bin Hao

doi:10.1007/s00343-010-9246-4

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Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease

OriginalPaper | Updated：2023-05-16

- Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease
- Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease
- 海洋湖沼学报（英文） 2010年28卷第1期页码：55-61
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Graduate University of Chinese Academy of Sciences
  3. Ocean University of China
- Author bio：
- Funds：
  
  Supported by the National Basic Research Program of China (973 Program) (No. 2006CB101803), the National Natural Science Foundation of China (No. 30871935), and the National High Technology Research and Development Program (863 Program) (No. 2003AA622070)
- DOI：10.1007/s00343-010-9246-4
  中图分类号：
- 网络首发：2010-01-27，
  
  纸质出版：2010
- Accepted：
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Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease[J]. 海洋湖沼学报（英文）, 2010,28(1):55-61.

Mo, Z., Guo, D., Mao, Y. et al. Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease., Chin. J. Ocean. Limnol. 28, 55–61 (2010). https://doi.org/10.1007/s00343-010-9246-4
Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease[J]. 海洋湖沼学报（英文）, 2010,28(1):55-61. DOI： 10.1007/s00343-010-9246-4.

Mo, Z., Guo, D., Mao, Y. et al. Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease., Chin. J. Ocean. Limnol. 28, 55–61 (2010). https://doi.org/10.1007/s00343-010-9246-4 DOI：

摘要

Abstract

We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain. This prtV gene encodes a putative protein of 918 amino acids

and is highly homologous to the V. cholerae prtV gene. We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar

lower protease activity against azocasein

and lower activity for four glycosidases. This prtV mutant strain also had increased activity for two esterases in its extracellular products

as analyzed by the API ZYM system. In addition

the prtV mutant strain exhibited decreased growth in turbot intestinal mucus and reduced hemolytic activity on turbot erythrocytes. Infection experiments showed that the LD50 of the prtV mutant strain increased by at least 1 log compared to the wild-type in turbot fish. We propose that prtV plays an important role in the pathogenesis of V. anguillarum.

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references

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