Cloning, characterization, and expression analysis of the DEAD-box family genes, Fc-vasa and Fc-PL10a, in Chinese shrimp (Fenneropenaeus chinensis)

Qianru Zhou; Mingyu Shao; Zhenkui Qin; Ho Kang Kyoung; Zhifeng Zhang

doi:10.1007/s00343-010-9231-y

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Cloning, characterization, and expression analysis of the DEAD-box family genes, Fc-vasa and Fc-PL10a, in Chinese shrimp (Fenneropenaeus chinensis)

OriginalPaper | Updated：2023-05-16

- Cloning, characterization, and expression analysis of the DEAD-box family genes, Fc-vasa and Fc-PL10a, in Chinese shrimp (Fenneropenaeus chinensis)
- Cloning, characterization, and expression analysis of the DEAD-box family genes, Fc-vasa and Fc-PL10a, in Chinese shrimp (Fenneropenaeus chinensis)
- 海洋湖沼学报（英文） 2010年28卷第1期页码：37-45
- Affiliations：
  
  1. Key Laboratory of Marine Genetics and Breeding of Ministry of Education Ocean University of China
  2. Department of Aquaculture Chonnam National University
- Author bio：
- Funds：
  
  Supported by the Specialized Research Fund for the Doctoral Program of Higher Education, China (No. 200804230015) and the National High Technology Research and Development Program of China (863 Program) (No. 2006AA10A401);An erratum to this article is available at;http://dx.doi.org/10.1007/s00343-010-0426-z;.
- DOI：10.1007/s00343-010-9231-y
  中图分类号：
- 网络首发：2010-01-27，
  
  纸质出版：2010
- Accepted：
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Cloning, characterization, and expression analysis of the DEAD-box family genes, Fc-vasa and Fc-PL10a, in Chinese shrimp (Fenneropenaeus chinensis)[J]. 海洋湖沼学报（英文）, 2010,28(1):37-45.

Zhou, Q., Shao, M., Qin, Z. et al. Cloning, characterization, and expression analysis of the DEAD-box family genes, Fc-vasa and Fc-PL10a, in Chinese shrimp (Fenneropenaeus chinensis)., Chin. J. Ocean. Limnol. 28, 37–45 (2010). https://doi.org/10.1007/s00343-010-9231-y
Cloning, characterization, and expression analysis of the DEAD-box family genes, Fc-vasa and Fc-PL10a, in Chinese shrimp (Fenneropenaeus chinensis)[J]. 海洋湖沼学报（英文）, 2010,28(1):37-45. DOI： 10.1007/s00343-010-9231-y.

Zhou, Q., Shao, M., Qin, Z. et al. Cloning, characterization, and expression analysis of the DEAD-box family genes, Fc-vasa and Fc-PL10a, in Chinese shrimp (Fenneropenaeus chinensis)., Chin. J. Ocean. Limnol. 28, 37–45 (2010). https://doi.org/10.1007/s00343-010-9231-y DOI：

摘要

Abstract

RNA helicases of the DEAD-box and related families are involved in various cellular processes including DNA replication

DNA repair

and RNA processing. However

the function of DEAD-box proteins in aquaculture species is poorly understood at molecular level. We obtained the full-length cDNA sequences of two genes encoding helicase-related proteins

Fc-vasa and Fc-PL10a

from the testes of Chinese shrimp

Fenneropenaeus chinensis. The two predicted amino acid sequences contain all the conserved motifs characterized by the DEAD-box family and several RGG repeats in the N-terminal regions. Homology and phylogenetic analyses indicate that they belong to the vasa and PL10 subfamilies. The three-dimensional structures of the two proteins were predicted with a homology modeling approach. Both core proteins consist of two tandem RecA-like domains similar to those of the DEAD-box RNA helicase. Using reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR we found that Fc-vasa was expressed specifically in the adult gonads. Transcription decreased in the ovary but increased in the testis during gonadal development. Fc-PL10a expression was widely distributed in the tissues we examined. Using in situ hybridization

we demonstrated that the Fc-vasa transcript is localized to the cytoplasm of the spermatogonia and oocytes. Thus

our results suggest that Fc-vasa plays an important role in germ-line development

and has utility as a germ cell lineage marker which will help to generate new insight into the origin and differentiation of germ cells as well as the regulation of reproduction in F. chinensis.

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references

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