Tissue culture of three species of Laurencia complex

Songdong Shen; Xunjian Wu; Binlun Yan; Lihong He

doi:10.1007/s00343-010-9134-y

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Tissue culture of three species of Laurencia complex

OriginalPaper | Updated：2023-05-16

- Tissue culture of three species of Laurencia complex
- Tissue culture of three species of Laurencia complex
- 海洋湖沼学报（英文） 2010年28卷第3期页码：514-520
- Affiliations：
  
  1. College of Life Sciences Soochow University
  2. Jiangsu Key Laboratory of Marine Biotechnology Huaihai Institute of Technology
- Author bio：
- Funds：
  
  Supported by the fund from Jiangsu Key Laboratory of Marine Biotechnology (No. 2006HS006), Huaihai Institute of Technology, Lianyungang, China;211-Project Funding of Soochow University (No. 14134908)
- DOI：10.1007/s00343-010-9134-y
  中图分类号：
- 网络首发：2010-05-20，
  
  纸质出版：2010
- Accepted：
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Tissue culture of three species of Laurencia complex[J]. 海洋湖沼学报（英文）, 2010,28(3):514-520.

Shen, S., Wu, X., Yan, B. et al. Tissue culture of three species of Laurencia complex., Chin. J. Ocean. Limnol. 28, 514–520 (2010). https://doi.org/10.1007/s00343-010-9134-y
Tissue culture of three species of Laurencia complex[J]. 海洋湖沼学报（英文）, 2010,28(3):514-520. DOI： 10.1007/s00343-010-9134-y.

Shen, S., Wu, X., Yan, B. et al. Tissue culture of three species of Laurencia complex., Chin. J. Ocean. Limnol. 28, 514–520 (2010). https://doi.org/10.1007/s00343-010-9134-y DOI：

摘要

Abstract

To establish a micropropagation system of three Laurencia complex species (Laurencia okamurai

Laurencia tristicha

and Chondrophycus undulatus) by tissue culture techniques

we studied the regeneration characteristics and optimal culture conditions of axenic algal fragments cultured on solid medium and in liquid medium. Regeneration structures were observed and counted regularly under a reverse microscope to investigate the regeneration process

polarity and optimal illumination

and temperature and salinity levels. The results show that in most cultures of the three species

we obtained bud regeneration on solidified medium with 0.5% agar and in liquid medium. Rhizoid-like regeneration was filamentous and developed from the lower cut surface of fragments in L. okamurai

but was discoid and developed from the apical back side of bud regeneration in L. tristicha and C. undulatus. Regeneration polarity was localized to the apical part of algal fronds in all three species

and on fragments cut from the basal part of algae buds could develop from both the upper and the lower cut surfaces. Buds could develop from both the medullary and the cortical portions in L. okamurai and C. undulatus

while in L. tristicha

buds only emerged from the cortex. The optimal culture conditions for L. okamurai were 4 500 lx

20°C and 35 (salinity); for C. undulatus

4 500 lx

20°C and 30; and for L. tristicha

4 500 lx

25°C and 30.

关键词

Keywords

references

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