Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation

Jinhua Li; Xin Ge; Xiaokui Zhang; Guanjun Chen; Yongxin Pan

doi:10.1007/s00343-010-9068-4

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Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation

OriginalPaper | Updated：2023-05-16

- Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation
- Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation
- 海洋湖沼学报（英文） 2010年28卷第4期页码：826-831
- Affiliations：
  
  1. State Key Laboratory of Microbial Technology Shandong University
  2. Biogeomagnetism Group Paleomagnetism and Geochronology Laboratory (SKL-LE) Institute of Geology and Geophysics Chinese Academy of Sciences
  3. Marine College Shandong University at Weihai
- Author bio：
- Funds：
  
  Supported by the Natural Science Foundation of Shandong Province, China (No. 2006ZRB01973), and the National Natural Science Foundation of China (Nos. 40821091, 40325011).
- DOI：10.1007/s00343-010-9068-4
  中图分类号：
- 网络首发：2010-06-29，
  
  纸质出版：2010
- Accepted：
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Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation[J]. 海洋湖沼学报（英文）, 2010,28(4):826-831.

Li, J., Ge, X., Zhang, X. et al. Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation., Chin. J. Ocean. Limnol. 28, 826–831 (2010). https://doi.org/10.1007/s00343-010-9068-4
Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation[J]. 海洋湖沼学报（英文）, 2010,28(4):826-831. DOI： 10.1007/s00343-010-9068-4.

Li, J., Ge, X., Zhang, X. et al. Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation., Chin. J. Ocean. Limnol. 28, 826–831 (2010). https://doi.org/10.1007/s00343-010-9068-4 DOI：

摘要

Abstract

Cultivable magnetotactic bacteria (MTB) in laboratory can provide sufficient samples for molecular microbiological and magnetic studies. However

a cold-stored MTB strain

such as Magnetospirillum magneticum AMB-1

often loses its ability to synthesize magnetosomes and consequently fails to sense the external magnetic field. It is therefore important to quickly recover vigorous bacteria cells that highly capable of magnetosome producing. In this study

a modified capillary magnetic separation system was designed to recover a deteriorating strain of Magnetospirillum magneticum AMB-1 that long-term cold-stored in a refrigerator. The results show that all cells obtained after a 3-cycle treatment were vigorous and had the ability to produce magnetosomes. Moreover

the 3rd-cycle recovered cells were able to form more magnetosome crystals. Compared with the colony formation method

this new method is time-saving

easily operated

and more efficient for recovering vigorous MTB cells.

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Keywords

references

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